Paracoccus halophilus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Paracoccus

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3457 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A099EVS8|A0A099EVS8_9RHOB Uncharacterized protein OS=Paracoccus halophilus OX=376733 GN=IT41_18345 PE=4 SV=1
MM1 pKa = 7.66TDD3 pKa = 3.21YY4 pKa = 8.29FTHH7 pKa = 6.94FSCLLDD13 pKa = 3.32VGTPEE18 pKa = 4.06NAASALDD25 pKa = 4.61LYY27 pKa = 11.2NALADD32 pKa = 4.43DD33 pKa = 4.82NAAEE37 pKa = 5.01DD38 pKa = 4.25PPSDD42 pKa = 3.7GFLLSIQPEE51 pKa = 4.26HH52 pKa = 6.99GGTQLWMRR60 pKa = 11.84DD61 pKa = 3.8DD62 pKa = 3.59VTGDD66 pKa = 3.66PEE68 pKa = 4.69HH69 pKa = 6.94VIQFVKK75 pKa = 10.27RR76 pKa = 11.84CARR79 pKa = 11.84EE80 pKa = 3.73FGLTGLWGMQYY91 pKa = 11.5ANTCSKK97 pKa = 10.44PRR99 pKa = 11.84VDD101 pKa = 3.8GFGGGAHH108 pKa = 6.1VLDD111 pKa = 4.82LATGEE116 pKa = 4.42TVDD119 pKa = 4.8WIYY122 pKa = 11.18TDD124 pKa = 3.52GWLSTVLGGGNPYY137 pKa = 10.08EE138 pKa = 4.12

Molecular weight:
14.98 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A099F0C1|A0A099F0C1_9RHOB DeoR faimly transcriptional regulator OS=Paracoccus halophilus OX=376733 GN=IT41_12430 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNLVRR13 pKa = 11.84ARR15 pKa = 11.84RR16 pKa = 11.84HH17 pKa = 4.37GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.12GGRR29 pKa = 11.84RR30 pKa = 11.84VLNARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.0GRR40 pKa = 11.84KK41 pKa = 8.91SLSAA45 pKa = 3.86

Molecular weight:
5.14 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3457

0

3457

1072070

36

2098

310.1

33.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.032 ± 0.062

0.829 ± 0.013

5.927 ± 0.035

5.739 ± 0.039

3.517 ± 0.029

8.973 ± 0.042

2.017 ± 0.02

5.33 ± 0.03

2.587 ± 0.03

10.276 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.785 ± 0.02

2.404 ± 0.026

5.379 ± 0.03

3.231 ± 0.026

7.699 ± 0.049

4.938 ± 0.026

4.992 ± 0.026

6.837 ± 0.032

1.426 ± 0.018

2.081 ± 0.02

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski