Micromonas pusilla virus 12T

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Algavirales; Phycodnaviridae; Prasinovirus; unclassified Prasinovirus

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 253 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4QRL7|M4QRL7_9PHYC Uncharacterized protein OS=Micromonas pusilla virus 12T OX=755272 GN=MPVG_00144 PE=4 SV=1
MM1 pKa = 7.57NMQTGFGDD9 pKa = 3.4SGDD12 pKa = 3.91NMAEE16 pKa = 4.11QYY18 pKa = 10.52ISTMMNIVTPVLEE31 pKa = 4.12KK32 pKa = 11.28SMVLACEE39 pKa = 3.92YY40 pKa = 11.13SKK42 pKa = 11.73ACGRR46 pKa = 11.84DD47 pKa = 2.8ILLPEE52 pKa = 5.1DD53 pKa = 3.13IEE55 pKa = 4.49YY56 pKa = 10.84ASKK59 pKa = 9.99YY60 pKa = 8.81CAMYY64 pKa = 9.8TVGHH68 pKa = 7.42DD69 pKa = 3.03IGSIFPEE76 pKa = 4.54IYY78 pKa = 10.14NDD80 pKa = 3.45QDD82 pKa = 3.72DD83 pKa = 4.47EE84 pKa = 4.54EE85 pKa = 5.48EE86 pKa = 3.91EE87 pKa = 4.55DD88 pKa = 4.17EE89 pKa = 5.07IEE91 pKa = 4.28EE92 pKa = 4.8VDD94 pKa = 5.7DD95 pKa = 4.8ADD97 pKa = 4.11CPLFTRR103 pKa = 11.84YY104 pKa = 10.19SGTEE108 pKa = 3.67EE109 pKa = 3.57RR110 pKa = 11.84FKK112 pKa = 10.84RR113 pKa = 11.84INEE116 pKa = 4.18AYY118 pKa = 10.18DD119 pKa = 3.95RR120 pKa = 11.84WDD122 pKa = 2.61SWQPQNPTEE131 pKa = 4.14VMLKK135 pKa = 10.42NAINSNGPSGMDD147 pKa = 2.97

Molecular weight: 16.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4QQE1|M4QQE1_9PHYC Uncharacterized protein OS=Micromonas pusilla virus 12T OX=755272 GN=MPVG_00234 PE=4 SV=1
MM1 pKa = 7.5LSSSSSRR8 pKa = 11.84RR9 pKa = 11.84ALRR12 pKa = 11.84ALFLDD17 pKa = 4.35HH18 pKa = 6.96PTHH21 pKa = 6.62AKK23 pKa = 9.75LVRR26 pKa = 11.84PQNPRR31 pKa = 11.84ATPPIVTKK39 pKa = 10.92LSTTTTPPRR48 pKa = 11.84PPRR51 pKa = 11.84PSGVEE56 pKa = 3.84YY57 pKa = 10.52IQDD60 pKa = 4.5GINDD64 pKa = 3.8RR65 pKa = 11.84RR66 pKa = 11.84LLPCKK71 pKa = 9.94HH72 pKa = 6.31LRR74 pKa = 11.84LLPCKK79 pKa = 9.47LLPVFPVHH87 pKa = 7.09LNVLVKK93 pKa = 10.43DD94 pKa = 3.94LQVVTEE100 pKa = 4.27LQVHH104 pKa = 5.72TFRR107 pKa = 11.84NLIRR111 pKa = 11.84VFIQLHH117 pKa = 4.44HH118 pKa = 7.26HH119 pKa = 5.53ITDD122 pKa = 4.46TIFNGHH128 pKa = 6.46LRR130 pKa = 11.84FPQPLVKK137 pKa = 10.31HH138 pKa = 6.24RR139 pKa = 11.84CTCRR143 pKa = 11.84LLFDD147 pKa = 3.91TSYY150 pKa = 10.7IHH152 pKa = 7.02YY153 pKa = 9.5IYY155 pKa = 10.2LGSKK159 pKa = 9.42SWVNDD164 pKa = 3.31HH165 pKa = 6.56SCWSSRR171 pKa = 11.84SSHH174 pKa = 4.4QTTLGGTLPLLRR186 pKa = 11.84GASFGSPQGSCYY198 pKa = 9.58PRR200 pKa = 11.84AVPP203 pKa = 5.08

Molecular weight: 23.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

253

0

253

62923

49

1674

248.7

28.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.384 ± 0.178

1.511 ± 0.118

6.06 ± 0.179

6.05 ± 0.241

4.253 ± 0.122

6.81 ± 0.389

2.074 ± 0.094

6.705 ± 0.121

7.369 ± 0.366

7.679 ± 0.181

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.722 ± 0.118

6.055 ± 0.236

4.143 ± 0.142

3.094 ± 0.092

4.61 ± 0.204

6.875 ± 0.205

6.921 ± 0.324

6.548 ± 0.138

1.054 ± 0.058

4.081 ± 0.133
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski