Salmonella phage SEN8

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Felsduovirus; Salmonella virus SEN8

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1S6KZY9|A0A1S6KZY9_9CAUD Endolysin OS=Salmonella phage SEN8 OX=1647468 GN=SEN8_11 PE=3 SV=1
MM1 pKa = 7.72KK2 pKa = 10.2KK3 pKa = 10.08YY4 pKa = 10.75QDD6 pKa = 3.13IKK8 pKa = 11.08NFRR11 pKa = 11.84LIDD14 pKa = 3.73APVNRR19 pKa = 11.84GKK21 pKa = 7.98TQSEE25 pKa = 4.38INIGAYY31 pKa = 9.83FLEE34 pKa = 5.32SEE36 pKa = 5.3DD37 pKa = 3.64GQDD40 pKa = 2.89WYY42 pKa = 10.7EE43 pKa = 4.14CQSLFSDD50 pKa = 3.76DD51 pKa = 3.73TAKK54 pKa = 10.43IMYY57 pKa = 9.57DD58 pKa = 3.26HH59 pKa = 7.22EE60 pKa = 4.48GVIWGVINKK69 pKa = 7.69PVPQRR74 pKa = 11.84GNTYY78 pKa = 9.8AVSMLWPVNMSVAEE92 pKa = 4.35IDD94 pKa = 5.01AADD97 pKa = 4.62CPDD100 pKa = 3.66DD101 pKa = 4.29CRR103 pKa = 11.84GDD105 pKa = 4.09GSWLYY110 pKa = 11.01RR111 pKa = 11.84DD112 pKa = 4.12GKK114 pKa = 9.77VLPVPVDD121 pKa = 3.49YY122 pKa = 10.47QAKK125 pKa = 10.73AEE127 pKa = 4.19TTRR130 pKa = 11.84QKK132 pKa = 11.31LLDD135 pKa = 3.97AANSVIADD143 pKa = 3.37WRR145 pKa = 11.84TEE147 pKa = 3.73LALGEE152 pKa = 4.44ISDD155 pKa = 4.67DD156 pKa = 4.32DD157 pKa = 4.18KK158 pKa = 12.09ASLTKK163 pKa = 8.87WMAYY167 pKa = 8.98IRR169 pKa = 11.84EE170 pKa = 4.38LKK172 pKa = 10.76SLVLTGISDD181 pKa = 3.44EE182 pKa = 4.2ATFNKK187 pKa = 9.4IQWPVLPQQ195 pKa = 3.32

Molecular weight:
22.07 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1S6KZZ3|A0A1S6KZZ3_9CAUD Major capsid protein OS=Salmonella phage SEN8 OX=1647468 GN=SEN8_6 PE=4 SV=1
MM1 pKa = 7.37KK2 pKa = 9.97KK3 pKa = 10.38KK4 pKa = 10.45VMSVFFQLAWAALLVISLLYY24 pKa = 9.29PRR26 pKa = 11.84SGAPVLVGASVWVSCFLAWLLAALCAVGWFAGDD59 pKa = 3.41RR60 pKa = 11.84ARR62 pKa = 11.84DD63 pKa = 3.54EE64 pKa = 4.01VRR66 pKa = 11.84AALIKK71 pKa = 10.38FRR73 pKa = 11.84AHH75 pKa = 5.83PVKK78 pKa = 10.2PVRR81 pKa = 11.84TWAIRR86 pKa = 11.84LLIVLCLAFSGWVITLVFYY105 pKa = 11.32LLTLVLYY112 pKa = 9.81QIARR116 pKa = 11.84SQLHH120 pKa = 6.13EE121 pKa = 4.36PMAAA125 pKa = 3.23

Molecular weight:
13.97 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

11156

39

935

227.7

25.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.537 ± 0.636

1.156 ± 0.163

5.791 ± 0.234

6.203 ± 0.363

3.155 ± 0.201

6.633 ± 0.422

1.936 ± 0.211

5.002 ± 0.243

5.208 ± 0.285

9.86 ± 0.294

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.591 ± 0.149

4.616 ± 0.296

4.213 ± 0.143

4.249 ± 0.212

6.517 ± 0.442

6.194 ± 0.189

5.88 ± 0.339

6.678 ± 0.279

1.721 ± 0.146

2.859 ± 0.234

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski