Allonocardiopsis opalescens

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Streptosporangiales incertae sedis; Allonocardiopsis

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6300 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T0PVL4|A0A2T0PVL4_9ACTN Uncharacterized protein OS=Allonocardiopsis opalescens OX=1144618 GN=CLV72_109195 PE=4 SV=1
MM1 pKa = 7.57KK2 pKa = 10.04IIHH5 pKa = 6.61ALIAVLAVAALTACAPLGAPPVADD29 pKa = 4.79LPPTEE34 pKa = 4.62PLADD38 pKa = 3.76EE39 pKa = 4.52ATVDD43 pKa = 3.37AAEE46 pKa = 4.35AAEE49 pKa = 4.4SPVEE53 pKa = 4.01EE54 pKa = 5.21GPAEE58 pKa = 4.28AEE60 pKa = 4.22LGSTHH65 pKa = 5.76TWNDD69 pKa = 2.7GLAIEE74 pKa = 4.53LSGVEE79 pKa = 3.79RR80 pKa = 11.84ALSGEE85 pKa = 4.13YY86 pKa = 10.68AMPAGEE92 pKa = 4.15PYY94 pKa = 10.85VRR96 pKa = 11.84FTMQVQNDD104 pKa = 3.56TGGPVDD110 pKa = 5.64LSLVWIEE117 pKa = 4.27CQVGEE122 pKa = 4.69DD123 pKa = 3.8GRR125 pKa = 11.84IGDD128 pKa = 4.54LVYY131 pKa = 10.68DD132 pKa = 4.06YY133 pKa = 11.48DD134 pKa = 5.46AGLGDD139 pKa = 4.36GFSSTVMDD147 pKa = 4.66GRR149 pKa = 11.84DD150 pKa = 3.16ATADD154 pKa = 3.98FGCAMPEE161 pKa = 4.0DD162 pKa = 4.2EE163 pKa = 6.28SYY165 pKa = 11.32LQIEE169 pKa = 4.36VHH171 pKa = 6.16PQDD174 pKa = 3.58EE175 pKa = 4.49AMLRR179 pKa = 11.84QPVFFIGDD187 pKa = 3.56VPP189 pKa = 3.78

Molecular weight: 19.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T0PU41|A0A2T0PU41_9ACTN Trk system potassium uptake protein TrkA OS=Allonocardiopsis opalescens OX=1144618 GN=CLV72_110179 PE=4 SV=1
MM1 pKa = 7.34NPAIHH6 pKa = 6.77RR7 pKa = 11.84RR8 pKa = 11.84HH9 pKa = 5.56TPALIPNSAPTPPKK23 pKa = 7.0TTPRR27 pKa = 11.84ASQQVAGIHH36 pKa = 5.21RR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84TRR42 pKa = 11.84PRR44 pKa = 11.84HH45 pKa = 4.87HH46 pKa = 6.97LTRR49 pKa = 11.84IPRR52 pKa = 11.84RR53 pKa = 11.84RR54 pKa = 11.84LHH56 pKa = 5.53PTRR59 pKa = 11.84LTRR62 pKa = 11.84PRR64 pKa = 11.84RR65 pKa = 11.84RR66 pKa = 11.84RR67 pKa = 11.84RR68 pKa = 11.84PRR70 pKa = 11.84LPTTPRR76 pKa = 11.84RR77 pKa = 11.84TRR79 pKa = 11.84RR80 pKa = 11.84PPTRR84 pKa = 11.84RR85 pKa = 11.84RR86 pKa = 11.84RR87 pKa = 11.84RR88 pKa = 11.84RR89 pKa = 11.84RR90 pKa = 11.84RR91 pKa = 11.84NRR93 pKa = 11.84RR94 pKa = 11.84RR95 pKa = 11.84RR96 pKa = 11.84RR97 pKa = 11.84HH98 pKa = 4.61LRR100 pKa = 11.84LRR102 pKa = 11.84RR103 pKa = 11.84HH104 pKa = 6.26HH105 pKa = 6.62EE106 pKa = 3.97PHH108 pKa = 6.54PHH110 pKa = 5.61PHH112 pKa = 6.57RR113 pKa = 11.84RR114 pKa = 11.84PHH116 pKa = 5.1QQNQQEE122 pKa = 4.6HH123 pKa = 6.06RR124 pKa = 11.84RR125 pKa = 11.84HH126 pKa = 5.99HH127 pKa = 5.6RR128 pKa = 11.84TQHH131 pKa = 4.87RR132 pKa = 11.84RR133 pKa = 11.84RR134 pKa = 11.84PRR136 pKa = 11.84LPLLTRR142 pKa = 11.84HH143 pKa = 5.83RR144 pKa = 11.84HH145 pKa = 4.95RR146 pKa = 11.84PHH148 pKa = 6.44PRR150 pKa = 11.84HH151 pKa = 5.23RR152 pKa = 11.84TPPRR156 pKa = 11.84RR157 pKa = 11.84RR158 pKa = 11.84RR159 pKa = 11.84LRR161 pKa = 11.84RR162 pKa = 11.84TRR164 pKa = 11.84PVRR167 pKa = 11.84RR168 pKa = 11.84IPRR171 pKa = 11.84PPHH174 pKa = 5.63PRR176 pKa = 11.84TRR178 pKa = 11.84PRR180 pKa = 11.84ARR182 pKa = 11.84QRR184 pKa = 11.84HH185 pKa = 6.26RR186 pKa = 11.84IQQPQRR192 pKa = 11.84PLTTRR197 pKa = 11.84RR198 pKa = 11.84RR199 pKa = 11.84PHH201 pKa = 5.38HH202 pKa = 4.47QTRR205 pKa = 11.84RR206 pKa = 11.84RR207 pKa = 11.84PRR209 pKa = 11.84RR210 pKa = 11.84TRR212 pKa = 11.84RR213 pKa = 11.84HH214 pKa = 5.06IRR216 pKa = 11.84GHH218 pKa = 4.61HH219 pKa = 6.29HH220 pKa = 7.44RR221 pKa = 11.84LTADD225 pKa = 3.63PLAPTPLHH233 pKa = 6.36RR234 pKa = 11.84RR235 pKa = 11.84SEE237 pKa = 4.22PVPRR241 pKa = 11.84RR242 pKa = 11.84GIARR246 pKa = 11.84RR247 pKa = 11.84TPPQQRR253 pKa = 11.84HH254 pKa = 4.54RR255 pKa = 11.84HH256 pKa = 4.48PTPRR260 pKa = 11.84GIHH263 pKa = 5.86PRR265 pKa = 11.84VHH267 pKa = 5.47HH268 pKa = 5.83TRR270 pKa = 11.84RR271 pKa = 11.84TLPQPAPQPVPTQLRR286 pKa = 11.84RR287 pKa = 11.84ITRR290 pKa = 11.84PQRR293 pKa = 11.84PTHH296 pKa = 5.15PHH298 pKa = 5.78PRR300 pKa = 11.84HH301 pKa = 6.05PARR304 pKa = 11.84PSAPPPTTRR313 pKa = 11.84RR314 pKa = 11.84TVVSQQRR321 pKa = 11.84HH322 pKa = 3.94RR323 pKa = 11.84QNRR326 pKa = 11.84ARR328 pKa = 11.84RR329 pKa = 11.84PRR331 pKa = 11.84AAAPTTGTDD340 pKa = 2.84VMRR343 pKa = 11.84RR344 pKa = 11.84GDD346 pKa = 3.42SS347 pKa = 3.16

Molecular weight: 42.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6300

0

6300

2018764

29

7340

320.4

34.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.891 ± 0.058

0.719 ± 0.008

5.954 ± 0.023

5.98 ± 0.03

2.676 ± 0.016

9.768 ± 0.033

2.143 ± 0.014

3.078 ± 0.02

1.207 ± 0.017

10.494 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.665 ± 0.013

1.541 ± 0.017

6.551 ± 0.029

2.429 ± 0.018

8.84 ± 0.041

4.63 ± 0.02

5.396 ± 0.024

8.51 ± 0.031

1.526 ± 0.013

2.001 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski