Microbacterium phage Neferthena

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Neferthenavirus; Microbacterium virus Neferthena

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385D4F6|A0A385D4F6_9CAUD Uncharacterized protein OS=Microbacterium phage Neferthena OX=2301539 GN=6 PE=4 SV=1
MM1 pKa = 8.27DD2 pKa = 6.24DD3 pKa = 4.8LNALLAPLPSYY14 pKa = 11.08AVLTDD19 pKa = 3.13QMKK22 pKa = 9.73QHH24 pKa = 6.86ALDD27 pKa = 3.58ISLVPDD33 pKa = 4.22AAGVWPGQPGYY44 pKa = 9.18VTTYY48 pKa = 10.47DD49 pKa = 3.9VYY51 pKa = 10.41WAALSLTGYY60 pKa = 10.05MMAQPFVKK68 pKa = 10.18QAASEE73 pKa = 4.48GTSTTVDD80 pKa = 3.28APNWSAITAYY90 pKa = 9.98FRR92 pKa = 11.84SQSPIACATTGAILTEE108 pKa = 4.3VPIPGLPHH116 pKa = 6.39VVPTDD121 pKa = 3.45MSGLGGDD128 pKa = 4.35GYY130 pKa = 11.66GDD132 pKa = 3.72VDD134 pKa = 3.8TDD136 pKa = 3.73LTT138 pKa = 4.02

Molecular weight: 14.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385D5C1|A0A385D5C1_9CAUD Uncharacterized protein OS=Microbacterium phage Neferthena OX=2301539 GN=54 PE=4 SV=1
MM1 pKa = 6.69NTAAPITSLRR11 pKa = 11.84QLSDD15 pKa = 3.42AQSHH19 pKa = 7.49AIRR22 pKa = 11.84SLGSVMKK29 pKa = 10.68YY30 pKa = 7.91RR31 pKa = 11.84TATEE35 pKa = 4.39LEE37 pKa = 4.17LSGRR41 pKa = 11.84TLMSLVEE48 pKa = 4.21LGLVEE53 pKa = 4.12MKK55 pKa = 10.2SYY57 pKa = 10.67RR58 pKa = 11.84VQSSNPRR65 pKa = 11.84ARR67 pKa = 11.84VSNEE71 pKa = 3.47PIIRR75 pKa = 11.84SYY77 pKa = 10.23RR78 pKa = 11.84NSMAGYY84 pKa = 8.34RR85 pKa = 11.84LARR88 pKa = 11.84KK89 pKa = 9.37GLL91 pKa = 3.61

Molecular weight: 10.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

13501

40

812

217.8

23.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.421 ± 0.341

0.667 ± 0.088

6.459 ± 0.4

5.903 ± 0.447

2.978 ± 0.17

8.155 ± 0.451

1.763 ± 0.18

5.037 ± 0.352

4.614 ± 0.312

8.296 ± 0.317

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.415 ± 0.177

3.4 ± 0.255

5.407 ± 0.221

4.311 ± 0.252

5.963 ± 0.467

5.422 ± 0.359

6.755 ± 0.382

7.318 ± 0.323

1.852 ± 0.165

2.866 ± 0.202
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski