Pacific flying fox faeces associated circular DNA virus-7

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 8.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140CTU3|A0A140CTU3_9VIRU Putative capsid protein OS=Pacific flying fox faeces associated circular DNA virus-7 OX=1796016 PE=4 SV=1
MM1 pKa = 7.24GADD4 pKa = 4.07GNHH7 pKa = 6.95HH8 pKa = 4.88YY9 pKa = 10.22QGYY12 pKa = 9.38IEE14 pKa = 4.61MPRR17 pKa = 11.84SVKK20 pKa = 8.9FTHH23 pKa = 6.5FKK25 pKa = 10.53CLAGAHH31 pKa = 5.68FEE33 pKa = 4.44KK34 pKa = 10.56RR35 pKa = 11.84WGTAQQASDD44 pKa = 3.73YY45 pKa = 11.32CNGLDD50 pKa = 4.28HH51 pKa = 7.29KK52 pKa = 11.25KK53 pKa = 10.4PGDD56 pKa = 3.47KK57 pKa = 10.64SIVIDD62 pKa = 5.25GPWIWGTMSKK72 pKa = 10.7GQGSRR77 pKa = 11.84TDD79 pKa = 3.38VLNLRR84 pKa = 11.84DD85 pKa = 3.59AVKK88 pKa = 9.99GGKK91 pKa = 9.25RR92 pKa = 11.84GRR94 pKa = 11.84EE95 pKa = 4.06LYY97 pKa = 10.83DD98 pKa = 4.48DD99 pKa = 4.17DD100 pKa = 4.58TVAGAAIKK108 pKa = 10.22FSRR111 pKa = 11.84GVAEE115 pKa = 4.05LTRR118 pKa = 11.84AYY120 pKa = 9.35STAPSRR126 pKa = 11.84GDD128 pKa = 3.03IRR130 pKa = 11.84CIFHH134 pKa = 7.03FGPPGTGKK142 pKa = 7.05THH144 pKa = 7.08CAHH147 pKa = 7.29DD148 pKa = 4.2DD149 pKa = 3.38KK150 pKa = 11.82GIFNINLTITLTLYY164 pKa = 9.37IAYY167 pKa = 10.19YY168 pKa = 10.6FDD170 pKa = 4.09GNNGGFWIGYY180 pKa = 8.05QGEE183 pKa = 4.17EE184 pKa = 3.88TAILDD189 pKa = 3.67EE190 pKa = 4.99FGGHH194 pKa = 5.6TLPPKK199 pKa = 9.99QLQRR203 pKa = 11.84LCDD206 pKa = 3.81KK207 pKa = 10.45YY208 pKa = 10.62PYY210 pKa = 9.48WMSIKK215 pKa = 10.24GGEE218 pKa = 4.43VACHH222 pKa = 5.79IRR224 pKa = 11.84TVHH227 pKa = 5.57ICSNYY232 pKa = 9.86LPSGWWSEE240 pKa = 3.67KK241 pKa = 9.38TLYY244 pKa = 11.0NEE246 pKa = 3.66DD247 pKa = 3.6AIYY250 pKa = 10.5RR251 pKa = 11.84RR252 pKa = 11.84IVEE255 pKa = 4.13VHH257 pKa = 4.26WHH259 pKa = 5.69YY260 pKa = 11.12EE261 pKa = 3.92FKK263 pKa = 10.69KK264 pKa = 9.45YY265 pKa = 10.51RR266 pKa = 11.84LYY268 pKa = 10.13KK269 pKa = 10.17TDD271 pKa = 4.4DD272 pKa = 4.3PLDD275 pKa = 3.82KK276 pKa = 10.57STWAMYY282 pKa = 10.63KK283 pKa = 9.98FLKK286 pKa = 10.64AKK288 pKa = 10.66LEE290 pKa = 4.08LEE292 pKa = 4.28YY293 pKa = 11.0TPIVHH298 pKa = 7.28KK299 pKa = 10.92

Molecular weight: 34.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140CTU3|A0A140CTU3_9VIRU Putative capsid protein OS=Pacific flying fox faeces associated circular DNA virus-7 OX=1796016 PE=4 SV=1
MM1 pKa = 7.54NGLQVRR7 pKa = 11.84QVGIFQSYY15 pKa = 10.57SFSQTPSGTVSVLPSSDD32 pKa = 3.27ANAVPIIIQNQSPSQLSSFINSIAATTDD60 pKa = 2.88TKK62 pKa = 11.18VYY64 pKa = 10.0IRR66 pKa = 11.84QVYY69 pKa = 8.41MKK71 pKa = 10.28GVYY74 pKa = 9.75YY75 pKa = 10.84NNSNTPIIMYY85 pKa = 9.53RR86 pKa = 11.84YY87 pKa = 9.42SFRR90 pKa = 11.84FRR92 pKa = 11.84KK93 pKa = 9.48NVATADD99 pKa = 3.77FASINAILTRR109 pKa = 11.84DD110 pKa = 3.62NPNINDD116 pKa = 4.04PLVNPFTSQIAQRR129 pKa = 11.84LMKK132 pKa = 10.42FGRR135 pKa = 11.84VKK137 pKa = 10.28AQYY140 pKa = 7.24MACGAMGTFKK150 pKa = 11.12LNNKK154 pKa = 9.1YY155 pKa = 10.82YY156 pKa = 10.47NPKK159 pKa = 10.1LVNQEE164 pKa = 4.16TEE166 pKa = 4.14GDD168 pKa = 4.15SVNYY172 pKa = 10.22LAMAGNKK179 pKa = 9.86CYY181 pKa = 9.8MFKK184 pKa = 9.9CTPVPIAHH192 pKa = 6.97FAGATTPVTILGTSWGSWSVSFNYY216 pKa = 9.49MSYY219 pKa = 10.48VSGYY223 pKa = 11.03VIGQNNPVTTYY234 pKa = 10.2VPPFIPTQTNKK245 pKa = 10.12YY246 pKa = 10.22FIFSDD251 pKa = 3.57QTGKK255 pKa = 9.33PQSTTNN261 pKa = 3.23

Molecular weight: 29.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

560

261

299

280.0

31.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.429 ± 0.057

1.786 ± 0.428

4.821 ± 1.44

2.857 ± 1.408

4.821 ± 0.623

8.214 ± 1.661

2.679 ± 1.546

6.607 ± 0.195

6.429 ± 1.233

5.357 ± 1.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.5 ± 0.638

5.893 ± 2.224

5.179 ± 0.899

4.107 ± 1.362

4.107 ± 0.702

6.786 ± 1.88

7.5 ± 0.884

5.536 ± 1.432

1.964 ± 0.807

6.429 ± 0.057
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski