Burkholderia virus phiE125

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Stanholtvirus

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8W6R1|Q8W6R1_9CAUD TnpA OS=Burkholderia virus phiE125 OX=180504 GN=tnpA PE=4 SV=1
MM1 pKa = 7.35NVTDD5 pKa = 3.97KK6 pKa = 11.35TKK8 pKa = 10.98GDD10 pKa = 3.4TSADD14 pKa = 3.37VTRR17 pKa = 11.84TVWSKK22 pKa = 11.35DD23 pKa = 3.28NEE25 pKa = 4.34FFSCDD30 pKa = 3.62ALDD33 pKa = 4.36EE34 pKa = 4.51LLDD37 pKa = 3.68MHH39 pKa = 7.54DD40 pKa = 4.03EE41 pKa = 4.13LAVGDD46 pKa = 3.95VVFYY50 pKa = 11.27GDD52 pKa = 3.53VAPIPTEE59 pKa = 4.02RR60 pKa = 11.84LCDD63 pKa = 3.92ADD65 pKa = 5.75DD66 pKa = 4.61VIDD69 pKa = 4.36MIGEE73 pKa = 4.11RR74 pKa = 11.84AYY76 pKa = 11.03DD77 pKa = 3.91EE78 pKa = 4.35VGEE81 pKa = 4.41AADD84 pKa = 5.22GYY86 pKa = 10.37PDD88 pKa = 3.56IAPEE92 pKa = 3.99AKK94 pKa = 10.54AEE96 pKa = 4.1LEE98 pKa = 4.22TLLSGWIEE106 pKa = 3.93KK107 pKa = 9.34HH108 pKa = 6.08AKK110 pKa = 7.05PTFYY114 pKa = 10.83SAANVNEE121 pKa = 4.04HH122 pKa = 6.7VITEE126 pKa = 4.23ANVEE130 pKa = 4.08GRR132 pKa = 11.84EE133 pKa = 4.09

Molecular weight: 14.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8W6R7|Q8W6R7_9CAUD Integrase OS=Burkholderia virus phiE125 OX=180504 GN=34 PE=3 SV=1
MM1 pKa = 7.92RR2 pKa = 11.84LPRR5 pKa = 11.84QTRR8 pKa = 11.84ARR10 pKa = 11.84RR11 pKa = 11.84AAVSSHH17 pKa = 6.02WISRR21 pKa = 11.84KK22 pKa = 8.68PRR24 pKa = 11.84SGFKK28 pKa = 10.1DD29 pKa = 3.11RR30 pKa = 11.84SMNEE34 pKa = 3.84RR35 pKa = 11.84LMTPADD41 pKa = 3.7LTVVTGKK48 pKa = 10.18KK49 pKa = 9.98RR50 pKa = 11.84YY51 pKa = 7.1TKK53 pKa = 10.17QADD56 pKa = 3.12WFKK59 pKa = 10.67ATFGINVVTAANGAVIMTWSTFEE82 pKa = 3.92SLQAKK87 pKa = 9.66KK88 pKa = 10.54AGLVGNPEE96 pKa = 3.96TSTVEE101 pKa = 3.95LCFDD105 pKa = 3.77

Molecular weight: 11.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

16002

43

1354

222.3

24.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.936 ± 0.798

1.181 ± 0.174

6.062 ± 0.198

6.143 ± 0.32

3.156 ± 0.179

8.086 ± 0.304

1.694 ± 0.168

4.706 ± 0.219

4.256 ± 0.26

7.937 ± 0.29

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.381 ± 0.123

3.287 ± 0.16

4.718 ± 0.362

4.056 ± 0.269

7.818 ± 0.304

5.881 ± 0.249

5.162 ± 0.205

7.28 ± 0.307

1.675 ± 0.16

2.587 ± 0.199
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski