Leptospira biflexa serovar Patoc (strain Patoc 1 / ATCC 23582 / Paris)

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Leptospirales; Leptospiraceae; Leptospira; Leptospira biflexa; Leptospira biflexa serovar Patoc

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3723 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B0SP40|B0SP40_LEPBP Uncharacterized protein OS=Leptospira biflexa serovar Patoc (strain Patoc 1 / ATCC 23582 / Paris) OX=456481 GN=LEPBI_I2984 PE=4 SV=1
MM1 pKa = 7.39GFIRR5 pKa = 11.84GQFGFVFKK13 pKa = 11.13LKK15 pKa = 8.8FTHH18 pKa = 6.44VLFLFTTLFVSCQSVTPVNLQMLFGSFFVSATGQGSVIYY57 pKa = 7.85EE58 pKa = 3.89APNFLFTSEE67 pKa = 4.16NGKK70 pKa = 8.72QAEE73 pKa = 4.26FNLRR77 pKa = 11.84LNMEE81 pKa = 4.71PNSSVRR87 pKa = 11.84IGPITISDD95 pKa = 3.64PTEE98 pKa = 3.78AVLVSDD104 pKa = 3.59TFINFTEE111 pKa = 4.2EE112 pKa = 4.07DD113 pKa = 3.54WNEE116 pKa = 3.6PHH118 pKa = 7.25IVRR121 pKa = 11.84LVGIDD126 pKa = 4.38DD127 pKa = 4.44PFSDD131 pKa = 4.0GNQNYY136 pKa = 8.94RR137 pKa = 11.84VQLGSILTSDD147 pKa = 3.51IRR149 pKa = 11.84FSTQSLPVLLVVNTDD164 pKa = 3.49NEE166 pKa = 4.6SSGVAASPVFGLLTSEE182 pKa = 4.18TGEE185 pKa = 4.24TGQISYY191 pKa = 10.73VLQTRR196 pKa = 11.84PMQDD200 pKa = 2.35VYY202 pKa = 11.5LRR204 pKa = 11.84NFVSSDD210 pKa = 3.05TTEE213 pKa = 3.89ATVEE217 pKa = 4.16SVEE220 pKa = 5.72LVFTPNNWDD229 pKa = 3.32VPQSVTVTGVDD240 pKa = 3.75DD241 pKa = 4.71FSVDD245 pKa = 3.03NSNFQISADD254 pKa = 3.59ATVSMDD260 pKa = 3.45PAYY263 pKa = 10.39LGKK266 pKa = 10.19VVPIITGTNVDD277 pKa = 3.17NDD279 pKa = 3.37IAGFTVVNLSGLTTTEE295 pKa = 3.49AGGAISFGVVLNTLPTHH312 pKa = 6.23SVTIPSIVATPTTEE326 pKa = 4.18SSISPTTLTFAPGEE340 pKa = 4.13WFTPKK345 pKa = 9.86IVTVTGVDD353 pKa = 3.45EE354 pKa = 5.46FIVDD358 pKa = 4.1GSQTVTIVSSAATSTDD374 pKa = 2.43SDD376 pKa = 4.67YY377 pKa = 11.81NGLAGPVFPSVTNTDD392 pKa = 2.77NDD394 pKa = 3.52VPGFVLTPPGSLSISEE410 pKa = 4.21NGGVLNFNIHH420 pKa = 6.91LSSQPPPGFTVTLTGINEE438 pKa = 4.18NNSITNVNTSSLVFTNANWNLDD460 pKa = 3.26QTIQITTNNNDD471 pKa = 2.71VDD473 pKa = 4.01EE474 pKa = 4.6DD475 pKa = 4.13TRR477 pKa = 11.84TVTLQFGSVDD487 pKa = 3.5TGGSADD493 pKa = 3.63PVYY496 pKa = 10.97NAIPPPTQVSFSVTDD511 pKa = 4.26DD512 pKa = 3.37DD513 pKa = 4.36TAGITVTPVGGLVVHH528 pKa = 6.67EE529 pKa = 4.8NGTPFTEE536 pKa = 4.4TFTVVLNSQPTQTVNIPTISSSNTAEE562 pKa = 3.98ITVSPSSLSFTTANWNTPQTVTLTSVLDD590 pKa = 4.21GSDD593 pKa = 5.68DD594 pKa = 3.48GDD596 pKa = 3.61QNVNINLSNTSSTDD610 pKa = 3.05PKK612 pKa = 10.76YY613 pKa = 11.01NSIVIASVTAINTDD627 pKa = 3.28SNEE630 pKa = 3.84PLVRR634 pKa = 11.84IQNLSASSIVEE645 pKa = 4.57DD646 pKa = 3.88GTSTITFEE654 pKa = 4.43IRR656 pKa = 11.84LSLKK660 pKa = 10.09PNANVTIGPITSSDD674 pKa = 3.07GTEE677 pKa = 3.53AVLLNSTSGVAASRR691 pKa = 11.84TLTFTPTNAQVANYY705 pKa = 9.81SGNTSEE711 pKa = 5.4SGWDD715 pKa = 3.52VPQTITIRR723 pKa = 11.84SVADD727 pKa = 3.46SFDD730 pKa = 4.14DD731 pKa = 4.17GNLPVTIHH739 pKa = 6.72IPQASGSYY747 pKa = 7.68FTGLYY752 pKa = 6.68PTGAVPGYY760 pKa = 9.43TDD762 pKa = 3.19TNGNLVVTVTDD773 pKa = 3.53NDD775 pKa = 4.07TKK777 pKa = 11.33GFTFSTTTLNLTEE790 pKa = 4.65GDD792 pKa = 3.19VDD794 pKa = 3.67GTFTVRR800 pKa = 11.84LNAAPCDD807 pKa = 4.06TPGNLAVCASGSVTIPISAEE827 pKa = 4.11TFSLPDD833 pKa = 3.26SAQYY837 pKa = 9.22TVSPANLTFTHH848 pKa = 6.49TNFVTPQTVTVTVVNDD864 pKa = 4.43SINEE868 pKa = 4.37SNTRR872 pKa = 11.84THH874 pKa = 6.46TLTLGAISGSGTDD887 pKa = 3.71YY888 pKa = 11.67EE889 pKa = 4.85GMNPSDD895 pKa = 3.54VTINITDD902 pKa = 3.74NDD904 pKa = 3.92NPSPKK909 pKa = 9.61ILFTLDD915 pKa = 2.58SGQPYY920 pKa = 7.75FTTEE924 pKa = 3.72SGFSTFYY931 pKa = 10.94SLRR934 pKa = 11.84LGSRR938 pKa = 11.84PIPGNSVTVTLSTSDD953 pKa = 3.3TTEE956 pKa = 3.76GMINDD961 pKa = 4.12SGTPASSKK969 pKa = 10.72QYY971 pKa = 10.83IFDD974 pKa = 3.51EE975 pKa = 5.05TNWSTSIPVEE985 pKa = 3.8IVGVLDD991 pKa = 3.68ILSDD995 pKa = 3.75GNINFNITVSGAEE1008 pKa = 4.11TGSFPSWYY1016 pKa = 9.99DD1017 pKa = 3.12SFVGSNGTTANLVNYY1032 pKa = 9.26SVSEE1036 pKa = 4.11NPVTVVTPQSMVRR1049 pKa = 11.84AEE1051 pKa = 3.95NAVAFSIYY1059 pKa = 10.06ILLSQAPTDD1068 pKa = 4.11DD1069 pKa = 3.32VTIPISISSSFPCTLFTGPTVSQFTLSTNTITLTSANWNTVGAHH1113 pKa = 4.56NTITVTPFDD1122 pKa = 5.13DD1123 pKa = 4.85SVDD1126 pKa = 4.07DD1127 pKa = 4.87GNVTCPIVVGNVSSSDD1143 pKa = 3.27GFFNGVNPFPSANYY1157 pKa = 9.8PEE1159 pKa = 4.78LTLNDD1164 pKa = 3.43NDD1166 pKa = 4.06AAGITTSGFSPSTVITSQSGASSEE1190 pKa = 4.61FYY1192 pKa = 10.37IHH1194 pKa = 7.13LNSQPTSDD1202 pKa = 3.43VTINLSAAPGGLVSFPTAPLTFTPSNFGTGQLVTILGQNTADD1244 pKa = 4.16LSDD1247 pKa = 3.53VNYY1250 pKa = 10.77NITPQITSSEE1260 pKa = 4.34SGTGFSPSTIYY1271 pKa = 10.65SALTPSTIPGVHH1283 pKa = 6.96IYY1285 pKa = 10.65NLYY1288 pKa = 10.69DD1289 pKa = 4.78IIPCTDD1295 pKa = 4.1PNPMVACGTSPNGSGGLVTSPNLVTSEE1322 pKa = 4.92LGAQSRR1328 pKa = 11.84FQLRR1332 pKa = 11.84FRR1334 pKa = 11.84ARR1336 pKa = 11.84PTSNVTIGVASSNASEE1352 pKa = 4.46GTTAVPNVTFTPTNWNTFQNIVITGVDD1379 pKa = 3.56DD1380 pKa = 4.22GLVDD1384 pKa = 4.23GNVLYY1389 pKa = 11.0SILFGSLTGGGTGFNGEE1406 pKa = 4.4SLPNVTVTNQDD1417 pKa = 2.5NDD1419 pKa = 3.38

Molecular weight: 149.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|B0SJC4|TRUA_LEPBP tRNA pseudouridine synthase A OS=Leptospira biflexa serovar Patoc (strain Patoc 1 / ATCC 23582 / Paris) OX=456481 GN=truA PE=3 SV=1
MM1 pKa = 7.75NSTTKK6 pKa = 10.29KK7 pKa = 9.79DD8 pKa = 3.31YY9 pKa = 10.34WVIGSLLFLSLVPSIAGAVRR29 pKa = 11.84IFQIATGSGYY39 pKa = 7.8TVEE42 pKa = 4.18NQRR45 pKa = 11.84FFNDD49 pKa = 4.94PIPVFVHH56 pKa = 6.39IISVLVYY63 pKa = 10.22SILGAFQFAPGFRR76 pKa = 11.84SRR78 pKa = 11.84HH79 pKa = 4.85LMWHH83 pKa = 5.76RR84 pKa = 11.84VSGRR88 pKa = 11.84FLVLFGLTSAISGVWLTLVYY108 pKa = 10.26PKK110 pKa = 10.76VPTDD114 pKa = 3.67GDD116 pKa = 3.73WLFGIRR122 pKa = 11.84MIVGVWMFLCVSLGFFFVWKK142 pKa = 10.2RR143 pKa = 11.84KK144 pKa = 8.43FQTHH148 pKa = 5.69SNWMLRR154 pKa = 11.84GYY156 pKa = 10.84AIGLGAGTQVFTHH169 pKa = 6.55LPWFVIVGGDD179 pKa = 3.47PSGVPRR185 pKa = 11.84DD186 pKa = 3.79LMMGAGWLINLIFAEE201 pKa = 3.9WLIRR205 pKa = 11.84RR206 pKa = 11.84KK207 pKa = 9.9KK208 pKa = 10.16

Molecular weight: 23.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3723

0

3723

1222038

17

2644

328.2

37.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.492 ± 0.038

0.797 ± 0.012

4.773 ± 0.025

7.052 ± 0.041

5.78 ± 0.041

6.481 ± 0.036

1.907 ± 0.019

7.621 ± 0.037

7.554 ± 0.043

10.419 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.16 ± 0.016

4.781 ± 0.031

4.075 ± 0.024

3.448 ± 0.019

4.116 ± 0.024

7.38 ± 0.036

5.342 ± 0.032

6.046 ± 0.032

1.135 ± 0.016

3.643 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski