Propionispora vibrioides

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Selenomonadales; Sporomusaceae; Propionispora

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4126 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H8TK23|A0A1H8TK23_9FIRM Predicted amino acid dehydrogenase OS=Propionispora vibrioides OX=112903 GN=SAMN04490178_1073 PE=4 SV=1
MM1 pKa = 6.96MRR3 pKa = 11.84ALYY6 pKa = 10.45SAVSGIKK13 pKa = 9.87AQQTSLDD20 pKa = 3.94VISNNIANVDD30 pKa = 3.45TTGYY34 pKa = 10.07KK35 pKa = 8.49SQRR38 pKa = 11.84VSFSDD43 pKa = 4.25LLSQTLSSASGSNGTSGGTNPVQVGLGTSVASTDD77 pKa = 3.39TIMTVGSSQSTGVATDD93 pKa = 4.01LSISGNGFFIVTGGSQSQYY112 pKa = 9.85QFTRR116 pKa = 11.84EE117 pKa = 3.47GDD119 pKa = 3.4LTYY122 pKa = 10.99DD123 pKa = 3.4ADD125 pKa = 4.54GNLTINGYY133 pKa = 8.37KK134 pKa = 8.66VCGWEE139 pKa = 4.67SYY141 pKa = 7.63TTDD144 pKa = 3.01SDD146 pKa = 3.94GNKK149 pKa = 9.78VYY151 pKa = 9.3NTNGAVEE158 pKa = 4.87PINLYY163 pKa = 10.45SDD165 pKa = 4.04SYY167 pKa = 11.41SGNKK171 pKa = 9.72KK172 pKa = 10.09LMAAKK177 pKa = 8.84ATTKK181 pKa = 8.74EE182 pKa = 4.32TLSGNLKK189 pKa = 7.7TTATAKK195 pKa = 9.21GTALNNIGTISQTATTKK212 pKa = 10.15TNSTGVSVSSASGLTQNSTYY232 pKa = 10.62TVTLTDD238 pKa = 3.82GTTSGHH244 pKa = 6.41FDD246 pKa = 2.96ITVTDD251 pKa = 3.79ASGATVATLTDD262 pKa = 3.61EE263 pKa = 4.81DD264 pKa = 4.91LSDD267 pKa = 3.78GATLTGSNGEE277 pKa = 4.51SIVLASNSSATAGTLTFTAANSKK300 pKa = 10.54DD301 pKa = 3.29QTTTMTVYY309 pKa = 10.51DD310 pKa = 4.21AQGNSYY316 pKa = 10.5DD317 pKa = 3.54VSVNFTKK324 pKa = 10.14SYY326 pKa = 9.7YY327 pKa = 10.41DD328 pKa = 3.12SSTGQTSWYY337 pKa = 8.67WEE339 pKa = 4.19TSSSDD344 pKa = 3.66SNVSSVTGSGYY355 pKa = 11.16LLFDD359 pKa = 3.34SSGNLVTTDD368 pKa = 2.69SSYY371 pKa = 11.49PSTASVTVSPSGSSPVTLSLDD392 pKa = 3.99FSNVASMVLSSGDD405 pKa = 3.49SSVTSSQDD413 pKa = 2.54GYY415 pKa = 11.82AAGTLSSLSISSDD428 pKa = 2.97GTITGTYY435 pKa = 10.65SNGQTQSLAQIALANFTNPQGLEE458 pKa = 3.96KK459 pKa = 11.01VGDD462 pKa = 3.87NLYY465 pKa = 9.05VTTVNSGAFTGGVVAGSNGTGSLSSGTLEE494 pKa = 3.98MSNVDD499 pKa = 4.06LAEE502 pKa = 3.99QFSNMMISQRR512 pKa = 11.84AYY514 pKa = 8.69QANSKK519 pKa = 10.54VITAADD525 pKa = 3.27QCLQSLINMVSS536 pKa = 2.82

Molecular weight: 54.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H8XL13|A0A1H8XL13_9FIRM Aspartate ammonia-lyase OS=Propionispora vibrioides OX=112903 GN=SAMN04490178_12547 PE=4 SV=1
MM1 pKa = 7.75RR2 pKa = 11.84EE3 pKa = 3.65NVRR6 pKa = 11.84WMTEE10 pKa = 3.85VALLAAFLSVAGAFKK25 pKa = 10.99LPGLLPGTEE34 pKa = 4.38FQLSAPLAVAICAVFGFAKK53 pKa = 10.65YY54 pKa = 8.25ITAGLLSSMAGLMLGTQSVWNVGIALIFRR83 pKa = 11.84CVVGAVFVLGGNRR96 pKa = 11.84WLTVVLAGPIATITSRR112 pKa = 11.84LVVGGILGNMAIPFMLAAVPGIIYY136 pKa = 8.09TAITVWPLTILLKK149 pKa = 10.68RR150 pKa = 11.84ITEE153 pKa = 4.1RR154 pKa = 11.84GKK156 pKa = 9.85KK157 pKa = 9.77AIVYY161 pKa = 7.51VVQRR165 pKa = 3.91

Molecular weight: 17.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4126

0

4126

1252127

25

2803

303.5

33.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.244 ± 0.05

1.155 ± 0.017

4.878 ± 0.027

6.315 ± 0.038

3.888 ± 0.03

7.544 ± 0.04

1.917 ± 0.019

7.037 ± 0.035

5.52 ± 0.038

10.296 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.636 ± 0.02

3.802 ± 0.034

3.892 ± 0.023

4.113 ± 0.027

4.823 ± 0.032

5.601 ± 0.036

5.5 ± 0.037

7.514 ± 0.032

0.972 ± 0.014

3.352 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski