Salmonella phage Skate

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 90 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z5HSQ4|A0A2Z5HSQ4_9CAUD Uncharacterized protein OS=Salmonella phage Skate OX=2234035 GN=CPT_Skate_045 PE=4 SV=1
MM1 pKa = 7.84IPLLWILSAYY11 pKa = 10.12AFARR15 pKa = 11.84VFEE18 pKa = 4.94ADD20 pKa = 3.03TLYY23 pKa = 11.42QMICYY28 pKa = 9.08GALFCLSGAALAFMDD43 pKa = 5.54DD44 pKa = 4.37VISDD48 pKa = 3.56

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z5HSR2|A0A2Z5HSR2_9CAUD Uncharacterized protein OS=Salmonella phage Skate OX=2234035 GN=CPT_Skate_055 PE=4 SV=1
MM1 pKa = 7.72GSTNGPSRR9 pKa = 11.84SRR11 pKa = 11.84ATGNTKK17 pKa = 9.72TGGKK21 pKa = 7.78TGAVKK26 pKa = 10.54PNGSTRR32 pKa = 11.84SPSRR36 pKa = 11.84GKK38 pKa = 10.36KK39 pKa = 9.35

Molecular weight: 3.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

90

0

90

15073

31

1096

167.5

18.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.492 ± 0.628

1.592 ± 0.176

5.978 ± 0.285

6.442 ± 0.276

3.377 ± 0.192

7.298 ± 0.263

1.858 ± 0.158

6.687 ± 0.206

6.76 ± 0.374

7.231 ± 0.207

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.019 ± 0.175

4.85 ± 0.239

3.344 ± 0.247

3.874 ± 0.34

5.42 ± 0.219

6.641 ± 0.238

5.347 ± 0.319

6.575 ± 0.247

1.632 ± 0.117

3.583 ± 0.234
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski