Anthoxanthum odoratum amalgavirus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Amalgaviridae; unclassified Amalgaviridae

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2V0A0Q4|A0A2V0A0Q4_9VIRU RNA-dependent RNA polymerase OS=Anthoxanthum odoratum amalgavirus 1 OX=2065797 GN=ORF1+ORF2 PE=4 SV=1
MM1 pKa = 7.78AEE3 pKa = 4.05PRR5 pKa = 11.84RR6 pKa = 11.84TFQTAAPTEE15 pKa = 4.09GDD17 pKa = 3.75YY18 pKa = 11.05VANLPADD25 pKa = 3.71EE26 pKa = 5.1DD27 pKa = 3.96AAFVYY32 pKa = 8.93FARR35 pKa = 11.84WVFTTYY41 pKa = 10.76HH42 pKa = 6.61LAAGLLDD49 pKa = 3.97PATYY53 pKa = 10.12RR54 pKa = 11.84PEE56 pKa = 4.71GYY58 pKa = 9.63SDD60 pKa = 3.77KK61 pKa = 11.68DD62 pKa = 3.01MAARR66 pKa = 11.84LRR68 pKa = 11.84FFKK71 pKa = 10.8GKK73 pKa = 9.4EE74 pKa = 3.55ADD76 pKa = 3.78VIDD79 pKa = 4.31TIFAVGVRR87 pKa = 11.84KK88 pKa = 10.29SFFTAKK94 pKa = 10.1EE95 pKa = 3.98SATFEE100 pKa = 3.89QFANFLEE107 pKa = 4.37FLRR110 pKa = 11.84TADD113 pKa = 3.01GAAAVTEE120 pKa = 4.11DD121 pKa = 3.51LRR123 pKa = 11.84RR124 pKa = 11.84SRR126 pKa = 11.84FQAAGKK132 pKa = 10.22GVFNAVEE139 pKa = 4.11ISSVAQLTVQLADD152 pKa = 4.05LEE154 pKa = 4.48LHH156 pKa = 5.46KK157 pKa = 10.52HH158 pKa = 5.18RR159 pKa = 11.84MSDD162 pKa = 3.25ASQKK166 pKa = 10.8RR167 pKa = 11.84IDD169 pKa = 3.98EE170 pKa = 4.52LEE172 pKa = 3.9LLIAKK177 pKa = 9.17EE178 pKa = 4.13KK179 pKa = 10.87EE180 pKa = 4.11SLEE183 pKa = 4.5TKK185 pKa = 10.57LKK187 pKa = 10.42MSDD190 pKa = 3.57EE191 pKa = 4.4EE192 pKa = 4.4FFPASIYY199 pKa = 10.84KK200 pKa = 10.18KK201 pKa = 9.96LNPMKK206 pKa = 10.13LQKK209 pKa = 10.2EE210 pKa = 4.24CWVRR214 pKa = 11.84AKK216 pKa = 10.81ASSTEE221 pKa = 3.93LAAVAVPTNDD231 pKa = 3.4QLADD235 pKa = 3.28ALKK238 pKa = 9.83NFKK241 pKa = 10.83EE242 pKa = 4.31EE243 pKa = 3.8VEE245 pKa = 4.14RR246 pKa = 11.84RR247 pKa = 11.84HH248 pKa = 6.53AVDD251 pKa = 6.31FLAQQNRR258 pKa = 11.84LTALRR263 pKa = 11.84EE264 pKa = 4.01YY265 pKa = 10.68VNKK268 pKa = 10.54KK269 pKa = 9.35ILSFRR274 pKa = 11.84ARR276 pKa = 11.84GKK278 pKa = 10.32YY279 pKa = 9.87RR280 pKa = 11.84IATRR284 pKa = 11.84LGHH287 pKa = 6.24VLAAMDD293 pKa = 4.1GQASGEE299 pKa = 4.17VPPAGAHH306 pKa = 6.42IGDD309 pKa = 4.59GDD311 pKa = 4.05DD312 pKa = 5.33SDD314 pKa = 5.43GEE316 pKa = 4.62DD317 pKa = 4.07YY318 pKa = 11.08IPDD321 pKa = 3.78YY322 pKa = 10.98EE323 pKa = 5.09RR324 pKa = 11.84EE325 pKa = 4.1LSDD328 pKa = 3.48EE329 pKa = 4.24SSGSDD334 pKa = 3.65EE335 pKa = 4.37EE336 pKa = 4.6SGSAGGTPEE345 pKa = 3.9RR346 pKa = 11.84HH347 pKa = 5.36RR348 pKa = 11.84TPTPARR354 pKa = 11.84SRR356 pKa = 11.84RR357 pKa = 11.84PRR359 pKa = 11.84RR360 pKa = 11.84RR361 pKa = 11.84RR362 pKa = 11.84DD363 pKa = 3.25YY364 pKa = 10.44TGLARR369 pKa = 11.84GSAPARR375 pKa = 11.84NTRR378 pKa = 11.84SRR380 pKa = 11.84KK381 pKa = 9.27AA382 pKa = 3.17

Molecular weight:
42.61 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2V0A0Q4|A0A2V0A0Q4_9VIRU RNA-dependent RNA polymerase OS=Anthoxanthum odoratum amalgavirus 1 OX=2065797 GN=ORF1+ORF2 PE=4 SV=1
MM1 pKa = 7.78AEE3 pKa = 4.05PRR5 pKa = 11.84RR6 pKa = 11.84TFQTAAPTEE15 pKa = 4.09GDD17 pKa = 3.75YY18 pKa = 11.05VANLPADD25 pKa = 3.71EE26 pKa = 5.1DD27 pKa = 3.96AAFVYY32 pKa = 8.93FARR35 pKa = 11.84WVFTTYY41 pKa = 10.76HH42 pKa = 6.61LAAGLLDD49 pKa = 3.97PATYY53 pKa = 10.12RR54 pKa = 11.84PEE56 pKa = 4.71GYY58 pKa = 9.63SDD60 pKa = 3.77KK61 pKa = 11.68DD62 pKa = 3.01MAARR66 pKa = 11.84LRR68 pKa = 11.84FFKK71 pKa = 10.8GKK73 pKa = 9.4EE74 pKa = 3.55ADD76 pKa = 3.78VIDD79 pKa = 4.31TIFAVGVRR87 pKa = 11.84KK88 pKa = 10.29SFFTAKK94 pKa = 10.1EE95 pKa = 3.98SATFEE100 pKa = 3.89QFANFLEE107 pKa = 4.37FLRR110 pKa = 11.84TADD113 pKa = 3.01GAAAVTEE120 pKa = 4.11DD121 pKa = 3.51LRR123 pKa = 11.84RR124 pKa = 11.84SRR126 pKa = 11.84FQAAGKK132 pKa = 10.22GVFNAVEE139 pKa = 4.11ISSVAQLTVQLADD152 pKa = 4.05LEE154 pKa = 4.48LHH156 pKa = 5.46KK157 pKa = 10.52HH158 pKa = 5.18RR159 pKa = 11.84MSDD162 pKa = 3.25ASQKK166 pKa = 10.8RR167 pKa = 11.84IDD169 pKa = 3.98EE170 pKa = 4.52LEE172 pKa = 3.9LLIAKK177 pKa = 9.17EE178 pKa = 4.13KK179 pKa = 10.87EE180 pKa = 4.11SLEE183 pKa = 4.5TKK185 pKa = 10.57LKK187 pKa = 10.42MSDD190 pKa = 3.57EE191 pKa = 4.4EE192 pKa = 4.4FFPASIYY199 pKa = 10.84KK200 pKa = 10.18KK201 pKa = 9.96LNPMKK206 pKa = 10.13LQKK209 pKa = 10.2EE210 pKa = 4.24CWVRR214 pKa = 11.84AKK216 pKa = 10.81ASSTEE221 pKa = 3.93LAAVAVPTNDD231 pKa = 3.4QLADD235 pKa = 3.28ALKK238 pKa = 9.83NFKK241 pKa = 10.83EE242 pKa = 4.31EE243 pKa = 3.8VEE245 pKa = 4.14RR246 pKa = 11.84RR247 pKa = 11.84HH248 pKa = 6.53AVDD251 pKa = 6.31FLAQQNRR258 pKa = 11.84LTALRR263 pKa = 11.84EE264 pKa = 4.01YY265 pKa = 10.68VNKK268 pKa = 10.52KK269 pKa = 9.13ILSFVLVEE277 pKa = 4.29NTGSRR282 pKa = 11.84PALDD286 pKa = 3.98TSWLPWTAKK295 pKa = 10.54LLEE298 pKa = 4.85KK299 pKa = 10.89YY300 pKa = 9.01PLPEE304 pKa = 5.16RR305 pKa = 11.84ISATAMIPMGRR316 pKa = 11.84TTSPTTSVSCRR327 pKa = 11.84TNLLEE332 pKa = 4.54VMRR335 pKa = 11.84SPALLAVRR343 pKa = 11.84QRR345 pKa = 11.84GTGPPPRR352 pKa = 11.84LGAGVPAAVGTTPVLQGGARR372 pKa = 11.84LHH374 pKa = 6.23VIRR377 pKa = 11.84APVRR381 pKa = 11.84PDD383 pKa = 2.86RR384 pKa = 11.84RR385 pKa = 11.84GIPYY389 pKa = 10.3ARR391 pKa = 11.84SKK393 pKa = 10.32WEE395 pKa = 3.79AGVRR399 pKa = 11.84KK400 pKa = 9.86IIGGGEE406 pKa = 3.87LSDD409 pKa = 3.45WQAASSKK416 pKa = 9.72VRR418 pKa = 11.84CGGNASDD425 pKa = 5.64ALLLLADD432 pKa = 4.48ASDD435 pKa = 3.86RR436 pKa = 11.84LPGKK440 pKa = 6.8TLRR443 pKa = 11.84GLFTLRR449 pKa = 11.84GARR452 pKa = 11.84DD453 pKa = 3.66ALRR456 pKa = 11.84LPSGLSVPDD465 pKa = 4.25GRR467 pKa = 11.84ACCNIKK473 pKa = 10.27HH474 pKa = 6.09FNVEE478 pKa = 3.64ATAGPFLRR486 pKa = 11.84AFGVKK491 pKa = 9.8KK492 pKa = 10.6KK493 pKa = 10.76EE494 pKa = 4.09SLEE497 pKa = 3.83QLLGDD502 pKa = 4.22FVWEE506 pKa = 4.52CFDD509 pKa = 6.04DD510 pKa = 4.87FATNDD515 pKa = 2.99ADD517 pKa = 3.83IRR519 pKa = 11.84RR520 pKa = 11.84LPFFGMRR527 pKa = 11.84IGFRR531 pKa = 11.84TKK533 pKa = 10.36LLKK536 pKa = 10.35KK537 pKa = 10.41SEE539 pKa = 4.32MLTKK543 pKa = 10.15IRR545 pKa = 11.84DD546 pKa = 3.9FKK548 pKa = 11.17PLGRR552 pKa = 11.84CVMMLDD558 pKa = 5.12AIEE561 pKa = 4.41QFCSSPLYY569 pKa = 10.56NVLSKK574 pKa = 10.97LSADD578 pKa = 3.55ALKK581 pKa = 11.07DD582 pKa = 3.45PMSGFRR588 pKa = 11.84NTAVRR593 pKa = 11.84ASSDD597 pKa = 2.64WSYY600 pKa = 9.73MWEE603 pKa = 4.09EE604 pKa = 3.54IKK606 pKa = 10.33EE607 pKa = 4.24AKK609 pKa = 10.17VCMEE613 pKa = 4.68LDD615 pKa = 2.81WSKK618 pKa = 10.93FDD620 pKa = 4.73RR621 pKa = 11.84EE622 pKa = 4.11RR623 pKa = 11.84PRR625 pKa = 11.84CDD627 pKa = 3.33LEE629 pKa = 4.55FMVDD633 pKa = 3.59VVISCFAPKK642 pKa = 10.29NEE644 pKa = 3.88RR645 pKa = 11.84EE646 pKa = 3.86QRR648 pKa = 11.84LLRR651 pKa = 11.84GYY653 pKa = 6.91EE654 pKa = 3.63VCMRR658 pKa = 11.84RR659 pKa = 11.84AIVEE663 pKa = 4.18RR664 pKa = 11.84VALLDD669 pKa = 4.29DD670 pKa = 4.4GALFEE675 pKa = 5.2IDD677 pKa = 3.61GMVPSGSLWTGWLDD691 pKa = 3.31TALNILYY698 pKa = 10.4LSAALSEE705 pKa = 4.86AGFSSYY711 pKa = 7.45MARR714 pKa = 11.84PKK716 pKa = 10.74CAGDD720 pKa = 3.9DD721 pKa = 3.7NLTLFMEE728 pKa = 4.81DD729 pKa = 3.42VPDD732 pKa = 3.63GRR734 pKa = 11.84LIQVRR739 pKa = 11.84GLLNDD744 pKa = 3.6WFLAGIKK751 pKa = 10.57DD752 pKa = 3.58EE753 pKa = 5.12DD754 pKa = 3.99FAITRR759 pKa = 11.84APFHH763 pKa = 6.8VEE765 pKa = 4.08TYY767 pKa = 10.35QAVFPPGTDD776 pKa = 3.29LTKK779 pKa = 10.06GTSKK783 pKa = 10.63IIDD786 pKa = 3.18QCEE789 pKa = 3.68WVRR792 pKa = 11.84FEE794 pKa = 4.82GQLQINQEE802 pKa = 4.01QGLSHH807 pKa = 6.82RR808 pKa = 11.84WQYY811 pKa = 10.66RR812 pKa = 11.84FKK814 pKa = 10.9GKK816 pKa = 9.57PKK818 pKa = 10.28FLSCYY823 pKa = 8.28WLADD827 pKa = 3.34GRR829 pKa = 11.84PIRR832 pKa = 11.84PASDD836 pKa = 3.13NVEE839 pKa = 3.83KK840 pKa = 10.78LLYY843 pKa = 10.0PEE845 pKa = 5.91GIHH848 pKa = 7.08KK849 pKa = 10.59SVEE852 pKa = 4.29DD853 pKa = 4.09YY854 pKa = 11.09ISACLAMVVDD864 pKa = 4.82NPFNQHH870 pKa = 5.29NVNHH874 pKa = 6.16MKK876 pKa = 10.09HH877 pKa = 6.55RR878 pKa = 11.84YY879 pKa = 9.24LIAQQIKK886 pKa = 9.37RR887 pKa = 11.84ILVTGIPDD895 pKa = 3.77RR896 pKa = 11.84LVLALARR903 pKa = 11.84IRR905 pKa = 11.84PDD907 pKa = 3.29GEE909 pKa = 3.6EE910 pKa = 4.66DD911 pKa = 2.97IPYY914 pKa = 10.64PMIAPWRR921 pKa = 11.84RR922 pKa = 11.84FKK924 pKa = 10.93EE925 pKa = 4.18YY926 pKa = 10.19IDD928 pKa = 4.17LDD930 pKa = 3.74SYY932 pKa = 11.77EE933 pKa = 5.42PVQQWIKK940 pKa = 10.9EE941 pKa = 3.92FDD943 pKa = 3.78DD944 pKa = 4.77FVAGISGLYY953 pKa = 9.33VRR955 pKa = 11.84STTGGIDD962 pKa = 2.88AYY964 pKa = 10.26KK965 pKa = 10.72FMDD968 pKa = 5.57FIRR971 pKa = 11.84GDD973 pKa = 3.5AVIGEE978 pKa = 4.5GQWGNEE984 pKa = 3.45MDD986 pKa = 2.9RR987 pKa = 11.84WIRR990 pKa = 11.84FITEE994 pKa = 4.03HH995 pKa = 5.98PVSRR999 pKa = 11.84SLRR1002 pKa = 11.84KK1003 pKa = 9.53ARR1005 pKa = 11.84RR1006 pKa = 11.84HH1007 pKa = 5.56NPASVPSAQVNQYY1020 pKa = 7.03YY1021 pKa = 9.79QRR1023 pKa = 11.84AAEE1026 pKa = 4.06GLNVYY1031 pKa = 9.69RR1032 pKa = 11.84QQLVNHH1038 pKa = 6.83RR1039 pKa = 11.84LDD1041 pKa = 3.01SSRR1044 pKa = 11.84EE1045 pKa = 3.72YY1046 pKa = 11.1GLWVSDD1052 pKa = 3.43LLRR1055 pKa = 11.84RR1056 pKa = 3.93

Molecular weight:
119.38 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1438

382

1056

719.0

81.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.848 ± 1.491

1.182 ± 0.454

6.676 ± 0.193

6.885 ± 0.735

5.076 ± 0.078

6.05 ± 0.143

1.53 ± 0.149

3.964 ± 0.663

5.563 ± 0.226

9.458 ± 0.791

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.156 ± 0.288

2.782 ± 0.21

4.798 ± 0.301

3.199 ± 0.157

8.623 ± 0.395

6.12 ± 0.209

4.798 ± 0.473

5.911 ± 0.462

1.599 ± 0.53

2.782 ± 0.048

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski