Raineyella sp. CBA3103

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Propionibacteriaceae; Raineyella; unclassified Raineyella

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2972 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2FAC0|A0A5Q2FAC0_9ACTN GNAT family N-acetyltransferase OS=Raineyella sp. CBA3103 OX=2662261 GN=Rai3103_08780 PE=4 SV=1
MM1 pKa = 6.69STIRR5 pKa = 11.84SGYY8 pKa = 9.59RR9 pKa = 11.84YY10 pKa = 10.07SEE12 pKa = 3.9DD13 pKa = 4.67HH14 pKa = 6.46EE15 pKa = 4.43WLDD18 pKa = 4.07TSASPARR25 pKa = 11.84VGISAVAAEE34 pKa = 4.04EE35 pKa = 4.34LGDD38 pKa = 3.98VVHH41 pKa = 7.15LEE43 pKa = 4.04LPEE46 pKa = 4.09VGAEE50 pKa = 4.08VTAGEE55 pKa = 4.17PCGEE59 pKa = 4.18VEE61 pKa = 4.38STKK64 pKa = 10.83SVADD68 pKa = 3.66IYY70 pKa = 11.39APVSGTVVEE79 pKa = 4.66INDD82 pKa = 4.09AVLDD86 pKa = 4.22DD87 pKa = 4.1PAVVNEE93 pKa = 4.37DD94 pKa = 3.7PYY96 pKa = 10.57GAGWLFSVEE105 pKa = 4.03VSTEE109 pKa = 3.82GSLLTAEE116 pKa = 5.12EE117 pKa = 4.33YY118 pKa = 10.96AEE120 pKa = 4.28KK121 pKa = 10.95NGGEE125 pKa = 4.2LTT127 pKa = 3.72

Molecular weight: 13.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2FC43|A0A5Q2FC43_9ACTN Pyridoxal kinase OS=Raineyella sp. CBA3103 OX=2662261 GN=pdxY PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.55LLKK22 pKa = 8.44KK23 pKa = 9.1TRR25 pKa = 11.84IQRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84AGKK33 pKa = 9.79

Molecular weight: 4.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2972

0

2972

955293

31

2256

321.4

34.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.789 ± 0.062

0.744 ± 0.011

6.066 ± 0.041

5.356 ± 0.046

2.82 ± 0.026

9.211 ± 0.043

2.237 ± 0.022

4.01 ± 0.03

1.918 ± 0.033

10.177 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.087 ± 0.02

1.849 ± 0.025

5.791 ± 0.036

2.968 ± 0.023

7.728 ± 0.049

5.327 ± 0.032

6.267 ± 0.041

8.994 ± 0.043

1.578 ± 0.02

2.083 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski