Human gut microviridae SH-CHD12

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X9Q116|A0A1X9Q116_9VIRU Putative VP1 OS=Human gut microviridae SH-CHD12 OX=1986032 PE=3 SV=1
MM1 pKa = 7.39IMSNIFSKK9 pKa = 10.57KK10 pKa = 8.87DD11 pKa = 3.27QYY13 pKa = 10.94IDD15 pKa = 3.21RR16 pKa = 11.84VNRR19 pKa = 11.84STFDD23 pKa = 3.37LSFVNNFTTNFGVITPVCLLPVGFGDD49 pKa = 4.7SVQINAKK56 pKa = 10.22FNLQLMPTVFPIQTQMYY73 pKa = 8.47ARR75 pKa = 11.84LHH77 pKa = 5.1FVYY80 pKa = 10.58VRR82 pKa = 11.84TRR84 pKa = 11.84TIWKK88 pKa = 9.14DD89 pKa = 2.92WQKK92 pKa = 10.76FFGGDD97 pKa = 3.32EE98 pKa = 4.33SVVPPFIAFNSQSKK112 pKa = 10.03LDD114 pKa = 4.13AMAQTSTLGDD124 pKa = 3.72YY125 pKa = 10.99LGLPTTIVGKK135 pKa = 8.64YY136 pKa = 9.34GRR138 pKa = 11.84YY139 pKa = 8.98AAYY142 pKa = 8.64PAYY145 pKa = 9.34TPRR148 pKa = 11.84EE149 pKa = 3.83WSYY152 pKa = 11.82VNPLSAIAIALNSVPVDD169 pKa = 3.41ADD171 pKa = 3.58ANALQTALNDD181 pKa = 3.59KK182 pKa = 9.46YY183 pKa = 11.64ASIAAAAPRR192 pKa = 11.84GDD194 pKa = 3.26EE195 pKa = 4.57GIYY198 pKa = 10.18GSAYY202 pKa = 8.81NWYY205 pKa = 10.62AFFTPATGQPFYY217 pKa = 10.65PSQNSDD223 pKa = 2.84NAYY226 pKa = 9.83EE227 pKa = 3.93FSFDD231 pKa = 3.06IDD233 pKa = 3.88FSRR236 pKa = 11.84TSGSLNNTEE245 pKa = 4.37FNNIAFISWAQEE257 pKa = 3.72NGDD260 pKa = 3.75MVVKK264 pKa = 10.29KK265 pKa = 10.47LSNIASASPNGNVTTWNVVIPSSEE289 pKa = 3.72VSGNVIRR296 pKa = 11.84SLALIYY302 pKa = 9.66PKK304 pKa = 10.49NSEE307 pKa = 4.35AIEE310 pKa = 4.07LVNIPANPSYY320 pKa = 10.98NNPWTTNFAFSGFLNVIDD338 pKa = 3.73TTEE341 pKa = 3.85QGKK344 pKa = 8.99VLLDD348 pKa = 3.21KK349 pKa = 11.14CPWYY353 pKa = 10.42DD354 pKa = 3.34GSTEE358 pKa = 4.27SKK360 pKa = 9.0TVRR363 pKa = 11.84ISALPFRR370 pKa = 11.84VYY372 pKa = 9.86EE373 pKa = 4.06AYY375 pKa = 10.99YY376 pKa = 10.28NAFGRR381 pKa = 11.84DD382 pKa = 3.28VRR384 pKa = 11.84NNPFMINGKK393 pKa = 9.8AEE395 pKa = 3.94YY396 pKa = 8.97NQYY399 pKa = 10.51VPTLDD404 pKa = 3.61SGADD408 pKa = 3.39GEE410 pKa = 4.71TYY412 pKa = 10.37EE413 pKa = 4.32LHH415 pKa = 6.1RR416 pKa = 11.84ANWEE420 pKa = 3.52PDD422 pKa = 3.03AYY424 pKa = 8.17TTALQSPQAGVAPLVGITSLGEE446 pKa = 3.7ATFRR450 pKa = 11.84DD451 pKa = 4.3ANGTTYY457 pKa = 9.92TAQLEE462 pKa = 4.35TAEE465 pKa = 5.43DD466 pKa = 4.55GDD468 pKa = 4.59TVTGFQMKK476 pKa = 10.45SSDD479 pKa = 3.42APADD483 pKa = 3.52VVRR486 pKa = 11.84NLIGMATSGISISDD500 pKa = 3.42FRR502 pKa = 11.84NVNSLQRR509 pKa = 11.84FLEE512 pKa = 3.62IRR514 pKa = 11.84IRR516 pKa = 11.84QSPRR520 pKa = 11.84YY521 pKa = 8.62LNLVKK526 pKa = 10.78GLFNVNLDD534 pKa = 3.53YY535 pKa = 11.51DD536 pKa = 4.42EE537 pKa = 6.67LMMPEE542 pKa = 4.27FLGGISDD549 pKa = 3.87NVPVYY554 pKa = 10.47KK555 pKa = 9.71VTQTTPTDD563 pKa = 3.92GSPLGSFAGQGFIQSGMKK581 pKa = 9.93HH582 pKa = 6.43VIRR585 pKa = 11.84KK586 pKa = 6.87YY587 pKa = 10.45CPEE590 pKa = 3.82EE591 pKa = 4.83GYY593 pKa = 10.43ILGVLSVVPAANYY606 pKa = 9.52SQLLAPHH613 pKa = 5.95WTRR616 pKa = 11.84SALLDD621 pKa = 3.1WHH623 pKa = 6.78FPQFNNISFQPMLYY637 pKa = 10.58KK638 pKa = 10.47NLCPLQAYY646 pKa = 8.88NNSPSSLSDD655 pKa = 3.13VYY657 pKa = 10.98GYY659 pKa = 10.31QRR661 pKa = 11.84AWWDD665 pKa = 4.02LISSFDD671 pKa = 3.7EE672 pKa = 3.7VHH674 pKa = 5.63GQFRR678 pKa = 11.84SSLRR682 pKa = 11.84NFVINRR688 pKa = 11.84VFDD691 pKa = 3.86TPPQLSKK698 pKa = 11.38DD699 pKa = 3.77FLLVDD704 pKa = 4.09PAQVNDD710 pKa = 3.62VFAVTAEE717 pKa = 4.15NGDD720 pKa = 4.12KK721 pKa = 10.7ILGSIAFDD729 pKa = 3.16ITKK732 pKa = 8.99KK733 pKa = 6.92TTIPRR738 pKa = 11.84NSIPHH743 pKa = 6.14IEE745 pKa = 3.93

Molecular weight: 82.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X9Q116|A0A1X9Q116_9VIRU Putative VP1 OS=Human gut microviridae SH-CHD12 OX=1986032 PE=3 SV=1
MM1 pKa = 7.58NPVLLLNRR9 pKa = 11.84SFTRR13 pKa = 11.84TNLKK17 pKa = 10.47LRR19 pKa = 11.84MFLPMLSFVSIFLCPPLCRR38 pKa = 11.84LLLLTVRR45 pKa = 11.84FRR47 pKa = 11.84TASLKK52 pKa = 10.42FIKK55 pKa = 10.42LSLKK59 pKa = 7.38MTKK62 pKa = 8.32QHH64 pKa = 6.48LYY66 pKa = 10.47KK67 pKa = 10.62VIEE70 pKa = 4.37VVLTAAISVAAILLLDD86 pKa = 3.89SCSASMSLFWKK97 pKa = 10.14NQGSSQRR104 pKa = 11.84TEE106 pKa = 3.65QSTSARR112 pKa = 11.84VDD114 pKa = 3.49TLKK117 pKa = 11.27SPDD120 pKa = 3.14INLNFF125 pKa = 4.12

Molecular weight: 14.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1703

125

745

425.8

48.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.34 ± 1.146

1.292 ± 0.691

5.637 ± 0.349

4.11 ± 0.427

4.932 ± 0.796

5.05 ± 0.754

1.527 ± 0.371

6.224 ± 0.247

4.991 ± 0.844

8.28 ± 1.078

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.29 ± 0.31

6.635 ± 0.733

4.932 ± 0.699

4.815 ± 0.754

5.05 ± 0.663

8.045 ± 0.916

5.755 ± 0.485

6.224 ± 0.488

1.585 ± 0.115

5.285 ± 1.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski