Mycobacterium phage OwlsT2W

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 103 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2R4AQH0|A0A2R4AQH0_9CAUD Uncharacterized protein OS=Mycobacterium phage OwlsT2W OX=2126954 GN=62 PE=4 SV=1
MM1 pKa = 7.27HH2 pKa = 7.18VSGPQCDD9 pKa = 3.35GGIPVGRR16 pKa = 11.84AQNVGATVRR25 pKa = 11.84PADD28 pKa = 3.81PVRR31 pKa = 11.84AAIQEE36 pKa = 4.24SLDD39 pKa = 3.61AQGDD43 pKa = 3.98GWQVAHH49 pKa = 5.88YY50 pKa = 8.9VAVVGLEE57 pKa = 4.16RR58 pKa = 11.84ITGDD62 pKa = 4.42RR63 pKa = 11.84MDD65 pKa = 5.24LGATTIITPVGQPDD79 pKa = 4.48YY80 pKa = 10.3LTEE83 pKa = 4.31GLVNRR88 pKa = 11.84YY89 pKa = 7.53WDD91 pKa = 3.72EE92 pKa = 4.72SDD94 pKa = 5.6DD95 pKa = 3.91EE96 pKa = 4.4

Molecular weight: 10.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2R4AQD0|A0A2R4AQD0_9CAUD Uncharacterized protein OS=Mycobacterium phage OwlsT2W OX=2126954 GN=81 PE=4 SV=1
MM1 pKa = 8.0PDD3 pKa = 3.22RR4 pKa = 11.84YY5 pKa = 10.94GEE7 pKa = 4.23TTPEE11 pKa = 3.57PRR13 pKa = 11.84VFVRR17 pKa = 11.84PKK19 pKa = 10.42VNALTVRR26 pKa = 11.84CSWCKK31 pKa = 9.72AGVGARR37 pKa = 11.84CVVAGTNLVLQRR49 pKa = 11.84SSFHH53 pKa = 5.62EE54 pKa = 3.84ARR56 pKa = 11.84VRR58 pKa = 11.84AAEE61 pKa = 3.98LAATGALTRR70 pKa = 11.84GRR72 pKa = 11.84MSS74 pKa = 2.99

Molecular weight: 8.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

103

0

103

18006

29

1162

174.8

19.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.646 ± 0.404

1.255 ± 0.188

6.559 ± 0.22

5.748 ± 0.254

2.86 ± 0.181

8.83 ± 0.573

2.227 ± 0.207

4.387 ± 0.213

3.421 ± 0.198

7.231 ± 0.251

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.221 ± 0.139

3.366 ± 0.151

6.126 ± 0.183

3.488 ± 0.195

6.581 ± 0.293

6.126 ± 0.303

6.881 ± 0.301

7.236 ± 0.272

2.238 ± 0.12

2.571 ± 0.168
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski