Candidatus Thiodiazotropha endoloripes

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Gammaproteobacteria incertae sedis; sulfur-oxidizing symbionts; Candidatus Thiodiazotropha

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3951 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E2UMW2|A0A1E2UMW2_9GAMM SirA family protein OS=Candidatus Thiodiazotropha endoloripes OX=1818881 GN=A3196_04095 PE=3 SV=1
MM1 pKa = 7.1LQKK4 pKa = 10.7KK5 pKa = 7.9SFRR8 pKa = 11.84LLALPASITAAILCSSLLLTGCDD31 pKa = 4.37GDD33 pKa = 5.47DD34 pKa = 4.43GDD36 pKa = 6.29DD37 pKa = 4.87GVDD40 pKa = 3.59GQNGVDD46 pKa = 3.85GTNGTDD52 pKa = 3.49GADD55 pKa = 3.91GITNYY60 pKa = 9.95VPLGLKK66 pKa = 10.17RR67 pKa = 11.84LATAPLDD74 pKa = 3.7AEE76 pKa = 4.42FTGLYY81 pKa = 10.23LNSDD85 pKa = 3.3NTLFLNVQHH94 pKa = 6.97PSSSNTTTDD103 pKa = 2.93AAGKK107 pKa = 10.06VFDD110 pKa = 4.64KK111 pKa = 10.56GTVGVIVGQDD121 pKa = 3.61FSALPEE127 pKa = 4.15NFGALDD133 pKa = 3.93LPVTTAQKK141 pKa = 9.62EE142 pKa = 4.4VVMTAVGSYY151 pKa = 9.07QVLAQQGDD159 pKa = 4.02SLDD162 pKa = 4.7DD163 pKa = 3.91GLAMGDD169 pKa = 3.19IMTADD174 pKa = 3.88GATQIKK180 pKa = 10.18SSNDD184 pKa = 2.96PDD186 pKa = 4.22FNGVVSDD193 pKa = 4.26GNGGFYY199 pKa = 10.61VYY201 pKa = 10.02TNWEE205 pKa = 4.19DD206 pKa = 3.81RR207 pKa = 11.84PGSMSRR213 pKa = 11.84IQVSGLTDD221 pKa = 3.03SGYY224 pKa = 11.52GSITQEE230 pKa = 3.82GMIDD234 pKa = 3.68FSGVGGTWVNCFGTVSPWNTPMSAEE259 pKa = 3.97EE260 pKa = 5.18LYY262 pKa = 10.95FDD264 pKa = 5.48DD265 pKa = 4.42TSDD268 pKa = 3.2WFNPDD273 pKa = 2.67YY274 pKa = 10.93EE275 pKa = 4.58YY276 pKa = 11.12FSNPQSLATYY286 pKa = 10.15LGYY289 pKa = 8.54PTDD292 pKa = 5.54GSGDD296 pKa = 3.11WPNPYY301 pKa = 9.47RR302 pKa = 11.84YY303 pKa = 10.2GYY305 pKa = 9.96IVEE308 pKa = 4.59IGNAADD314 pKa = 3.34AAVANVTVNKK324 pKa = 9.95LEE326 pKa = 4.06TMGRR330 pKa = 11.84FSHH333 pKa = 6.23EE334 pKa = 4.23NSVVMPDD341 pKa = 3.77DD342 pKa = 3.42KK343 pKa = 11.34TVFLSDD349 pKa = 4.01DD350 pKa = 3.46GTGVVFFKK358 pKa = 10.27FVADD362 pKa = 3.67VAGDD366 pKa = 3.62MSAGTLYY373 pKa = 10.81AAQITQAAGVDD384 pKa = 4.12DD385 pKa = 4.41PAEE388 pKa = 4.02AALGIEE394 pKa = 4.42WIEE397 pKa = 3.86LASMGEE403 pKa = 4.11AEE405 pKa = 4.74IEE407 pKa = 3.79AAIASFDD414 pKa = 3.53GTFADD419 pKa = 4.34GNYY422 pKa = 8.23ITDD425 pKa = 3.68EE426 pKa = 4.21QVCDD430 pKa = 3.3WAEE433 pKa = 4.23SKK435 pKa = 10.81SGADD439 pKa = 3.59LSCDD443 pKa = 3.11EE444 pKa = 5.18DD445 pKa = 3.96VTVDD449 pKa = 4.12ANPFSDD455 pKa = 5.13DD456 pKa = 2.86RR457 pKa = 11.84VAYY460 pKa = 10.57LEE462 pKa = 4.0SRR464 pKa = 11.84KK465 pKa = 10.06AAVALGATGEE475 pKa = 4.01FRR477 pKa = 11.84KK478 pKa = 9.65MEE480 pKa = 4.13GVNINYY486 pKa = 9.83NLASTWWNGGAADD499 pKa = 4.17GDD501 pKa = 3.75QAYY504 pKa = 8.6MYY506 pKa = 9.68MAMSSFDD513 pKa = 3.18ATMSDD518 pKa = 4.32DD519 pKa = 3.72EE520 pKa = 5.66GAIQLNGDD528 pKa = 3.54NGKK531 pKa = 10.38CGVVYY536 pKa = 10.59RR537 pKa = 11.84MKK539 pKa = 11.06LMRR542 pKa = 11.84NAAGEE547 pKa = 4.28VDD549 pKa = 4.28VMTMVPAIVGGPYY562 pKa = 10.13YY563 pKa = 10.88ADD565 pKa = 3.35RR566 pKa = 11.84SVNEE570 pKa = 4.25CNVNNISNPDD580 pKa = 3.48NLLIMDD586 pKa = 5.51DD587 pKa = 3.56GRR589 pKa = 11.84VLIGEE594 pKa = 4.12DD595 pKa = 3.21TGNHH599 pKa = 5.36EE600 pKa = 4.66NNVVWVFDD608 pKa = 4.2DD609 pKa = 3.86PAII612 pKa = 4.35

Molecular weight: 65.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E2UMA6|A0A1E2UMA6_9GAMM DNA-binding response regulator OS=Candidatus Thiodiazotropha endoloripes OX=1818881 GN=A3196_03395 PE=4 SV=1
MM1 pKa = 7.38YY2 pKa = 8.15MAKK5 pKa = 10.13KK6 pKa = 10.0KK7 pKa = 9.87AAKK10 pKa = 9.88KK11 pKa = 9.94KK12 pKa = 8.93ATAKK16 pKa = 10.47RR17 pKa = 11.84ATTTKK22 pKa = 10.13KK23 pKa = 10.04VAVKK27 pKa = 10.25KK28 pKa = 10.36RR29 pKa = 11.84ATKK32 pKa = 10.51KK33 pKa = 10.26KK34 pKa = 8.43VATKK38 pKa = 10.53KK39 pKa = 10.29RR40 pKa = 11.84VATKK44 pKa = 10.57KK45 pKa = 10.23KK46 pKa = 8.21ATKK49 pKa = 10.23KK50 pKa = 10.18KK51 pKa = 9.16VAAKK55 pKa = 10.11KK56 pKa = 9.73RR57 pKa = 11.84ATKK60 pKa = 10.33KK61 pKa = 10.33RR62 pKa = 11.84VVKK65 pKa = 10.75KK66 pKa = 10.33KK67 pKa = 8.67ATKK70 pKa = 10.36KK71 pKa = 10.31KK72 pKa = 8.82VAKK75 pKa = 10.34KK76 pKa = 10.05KK77 pKa = 8.6ATKK80 pKa = 10.36KK81 pKa = 10.21KK82 pKa = 8.61VAKK85 pKa = 10.37KK86 pKa = 9.94KK87 pKa = 8.69VAKK90 pKa = 10.41KK91 pKa = 10.0KK92 pKa = 8.69VAKK95 pKa = 10.41KK96 pKa = 10.17KK97 pKa = 8.76VAKK100 pKa = 10.36KK101 pKa = 10.09KK102 pKa = 8.6ATKK105 pKa = 10.36KK106 pKa = 10.21KK107 pKa = 8.61VAKK110 pKa = 10.37KK111 pKa = 10.13KK112 pKa = 8.76VAKK115 pKa = 10.36KK116 pKa = 10.28KK117 pKa = 8.77ATKK120 pKa = 10.18KK121 pKa = 9.97KK122 pKa = 9.18AAKK125 pKa = 9.94KK126 pKa = 9.89KK127 pKa = 9.0VAAKK131 pKa = 9.84KK132 pKa = 10.2RR133 pKa = 11.84PARR136 pKa = 11.84PRR138 pKa = 11.84KK139 pKa = 7.44VTPRR143 pKa = 11.84KK144 pKa = 9.86KK145 pKa = 10.11KK146 pKa = 9.98AASS149 pKa = 3.22

Molecular weight: 16.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3951

0

3951

1269643

51

2344

321.3

35.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.523 ± 0.039

0.988 ± 0.018

5.807 ± 0.034

6.664 ± 0.042

3.711 ± 0.022

7.324 ± 0.04

2.338 ± 0.023

6.067 ± 0.033

4.326 ± 0.032

10.963 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.62 ± 0.019

3.634 ± 0.03

4.392 ± 0.027

4.664 ± 0.035

5.712 ± 0.034

6.431 ± 0.028

5.043 ± 0.035

6.648 ± 0.033

1.285 ± 0.016

2.858 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski