Miniimonas arenae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Beutenbergiaceae; Miniimonas

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2846 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C5BG52|A0A5C5BG52_9MICO UDP-N-acetylmuramyl-tripeptide synthetase OS=Miniimonas arenae OX=676201 GN=murE PE=3 SV=1
MM1 pKa = 7.73RR2 pKa = 11.84KK3 pKa = 9.48SLLSVAAVASVTLLLTACGGGGGGTDD29 pKa = 3.18ATGDD33 pKa = 3.63STATTDD39 pKa = 3.74ASAEE43 pKa = 4.05ATGDD47 pKa = 3.4AGASADD53 pKa = 3.64PSAPPVRR60 pKa = 11.84GNEE63 pKa = 4.08DD64 pKa = 3.54LVVWTDD70 pKa = 3.22ATKK73 pKa = 10.88LDD75 pKa = 3.76AVTAVAQAFGEE86 pKa = 4.38ANGITVGVQAIVNTRR101 pKa = 11.84SDD103 pKa = 4.58FITANQAGNGPDD115 pKa = 3.68VLVGAHH121 pKa = 6.58DD122 pKa = 4.43WIGQLVANGAIDD134 pKa = 4.08PLQISADD141 pKa = 3.79SLSGYY146 pKa = 7.39SEE148 pKa = 4.29KK149 pKa = 10.9AVTAVTYY156 pKa = 10.22NGQLMGIPYY165 pKa = 10.04GVEE168 pKa = 3.95SLVLYY173 pKa = 10.17CNKK176 pKa = 9.73TYY178 pKa = 11.02APDD181 pKa = 3.64TYY183 pKa = 10.94ATLDD187 pKa = 3.54DD188 pKa = 5.75AIAAGQAAKK197 pKa = 10.5DD198 pKa = 3.57AGSVQSALNIPQGVNGDD215 pKa = 4.13PYY217 pKa = 11.28HH218 pKa = 5.67MQPLFTSAGGYY229 pKa = 10.5LFGLDD234 pKa = 3.3ATGTPDD240 pKa = 4.71ANDD243 pKa = 3.86LGVGTDD249 pKa = 3.43AGIAAAQKK257 pKa = 10.18IATLGDD263 pKa = 3.58AGSKK267 pKa = 9.98VLSTAITNDD276 pKa = 3.01NAISLFAQGQAGCLISGPWALSDD299 pKa = 3.78VEE301 pKa = 4.94AGLGADD307 pKa = 4.83GYY309 pKa = 8.47TVQPIPGFAGMSPAEE324 pKa = 4.16PFMGAQAFYY333 pKa = 10.77VASNGQNKK341 pKa = 9.54SFAQAFVTGTSGGGLNSEE359 pKa = 4.59EE360 pKa = 4.3AMTTLFEE367 pKa = 4.42GTNLPPAMTSVRR379 pKa = 11.84ATAAASDD386 pKa = 3.94PLIGVFGDD394 pKa = 3.9AADD397 pKa = 3.76AAYY400 pKa = 9.63PMPAIPAMDD409 pKa = 3.8QVWTPLGQAYY419 pKa = 9.89AAIIGGADD427 pKa = 3.63PASTMTTAAQTISSAIASSS446 pKa = 3.24

Molecular weight: 44.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C5BDP5|A0A5C5BDP5_9MICO Prepilin peptidase OS=Miniimonas arenae OX=676201 GN=FH969_05510 PE=3 SV=1
MM1 pKa = 7.13GRR3 pKa = 11.84AGAGGSGRR11 pKa = 11.84AGVRR15 pKa = 11.84AGRR18 pKa = 11.84RR19 pKa = 11.84AGGRR23 pKa = 11.84GAGRR27 pKa = 11.84RR28 pKa = 11.84GRR30 pKa = 11.84GGQVRR35 pKa = 11.84TGRR38 pKa = 11.84GRR40 pKa = 11.84APTARR45 pKa = 11.84AANNGGGFGAIRR57 pKa = 11.84SEE59 pKa = 3.9TAAIVGRR66 pKa = 11.84RR67 pKa = 11.84RR68 pKa = 11.84TGARR72 pKa = 11.84SMLRR76 pKa = 11.84GRR78 pKa = 11.84FRR80 pKa = 11.84SAALRR85 pKa = 11.84KK86 pKa = 9.26RR87 pKa = 11.84PHH89 pKa = 6.08SARR92 pKa = 11.84ASYY95 pKa = 9.23GHH97 pKa = 6.9RR98 pKa = 11.84PHH100 pKa = 7.04ARR102 pKa = 11.84SQSRR106 pKa = 11.84RR107 pKa = 11.84AALGKK112 pKa = 10.03RR113 pKa = 11.84PRR115 pKa = 11.84KK116 pKa = 7.39RR117 pKa = 11.84TRR119 pKa = 11.84PATPRR124 pKa = 11.84RR125 pKa = 11.84TEE127 pKa = 3.53VRR129 pKa = 11.84RR130 pKa = 11.84SGRR133 pKa = 11.84TRR135 pKa = 3.0

Molecular weight: 14.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2846

0

2846

974644

26

3032

342.5

36.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.834 ± 0.077

0.534 ± 0.011

6.298 ± 0.044

5.516 ± 0.046

2.475 ± 0.029

9.462 ± 0.043

2.078 ± 0.025

3.05 ± 0.039

1.313 ± 0.027

10.316 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.513 ± 0.021

1.471 ± 0.024

6.039 ± 0.04

2.551 ± 0.027

7.788 ± 0.061

5.157 ± 0.042

6.377 ± 0.053

9.904 ± 0.054

1.55 ± 0.021

1.774 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski