Brevibacillus laterosporus LMG 15441

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Brevibacillus; Brevibacillus laterosporus

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4532 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A075R5L5|A0A075R5L5_BRELA dTDP-glucose 4 6-dehydratase OS=Brevibacillus laterosporus LMG 15441 OX=1042163 GN=BRLA_c035750 PE=3 SV=1
MM1 pKa = 7.59TKK3 pKa = 10.25PEE5 pKa = 4.08WVFDD9 pKa = 3.88SSWRR13 pKa = 11.84DD14 pKa = 3.28DD15 pKa = 3.66PRR17 pKa = 11.84YY18 pKa = 9.85HH19 pKa = 7.73LSEE22 pKa = 5.96DD23 pKa = 3.14ILMAEE28 pKa = 4.84PDD30 pKa = 3.01WSIQYY35 pKa = 8.53TCITSDD41 pKa = 4.27PGWSDD46 pKa = 3.55DD47 pKa = 3.83PGYY50 pKa = 11.45

Molecular weight: 5.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A075R8Q5|A0A075R8Q5_BRELA MerR HTH family regulatory protein OS=Brevibacillus laterosporus LMG 15441 OX=1042163 GN=BRLA_07p000070 PE=4 SV=1
MM1 pKa = 7.88RR2 pKa = 11.84PTFNPNNRR10 pKa = 11.84KK11 pKa = 9.36RR12 pKa = 11.84KK13 pKa = 8.62KK14 pKa = 9.81DD15 pKa = 2.98HH16 pKa = 6.23GFRR19 pKa = 11.84KK20 pKa = 10.03RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.91VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4532

0

4532

1432094

30

12190

316.0

35.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.28 ± 0.038

0.838 ± 0.012

4.915 ± 0.028

7.147 ± 0.044

4.198 ± 0.028

6.703 ± 0.038

2.289 ± 0.018

7.306 ± 0.031

6.302 ± 0.042

9.997 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.867 ± 0.018

3.92 ± 0.023

3.846 ± 0.024

4.532 ± 0.037

4.497 ± 0.028

6.059 ± 0.031

5.68 ± 0.033

7.026 ± 0.03

1.073 ± 0.013

3.524 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski