Streptococcus phage phi891591

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1PFT0|M1PFT0_9CAUD Putative HNH endonuclease OS=Streptococcus phage phi891591 OX=1289599 GN=phi891591_0026 PE=4 SV=1
MM1 pKa = 7.37KK2 pKa = 10.36KK3 pKa = 10.44EE4 pKa = 4.15EE5 pKa = 3.99IKK7 pKa = 10.79QYY9 pKa = 11.22LDD11 pKa = 3.0VDD13 pKa = 3.89LEE15 pKa = 4.44FYY17 pKa = 11.49YY18 pKa = 10.73NGQGACFLPSICVVGYY34 pKa = 10.04DD35 pKa = 4.37NKK37 pKa = 10.79GQQFDD42 pKa = 4.68SIDD45 pKa = 3.18KK46 pKa = 11.36AMDD49 pKa = 3.49AKK51 pKa = 11.25VFDD54 pKa = 4.52GKK56 pKa = 11.05SLVDD60 pKa = 3.15IWDD63 pKa = 3.48EE64 pKa = 3.99VLPQVSS70 pKa = 3.26

Molecular weight: 7.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1PL99|M1PL99_9CAUD Uncharacterized protein OS=Streptococcus phage phi891591 OX=1289599 GN=phi891591_0030 PE=4 SV=1
MM1 pKa = 7.28QNLKK5 pKa = 10.49GGRR8 pKa = 11.84TPPIRR13 pKa = 11.84SAGLHH18 pKa = 5.54AVTVHH23 pKa = 5.96FFSRR27 pKa = 11.84YY28 pKa = 9.82DD29 pKa = 3.23LGGRR33 pKa = 11.84KK34 pKa = 7.79WEE36 pKa = 3.97KK37 pKa = 10.94LNVKK41 pKa = 10.07SVVNHH46 pKa = 6.34LSEE49 pKa = 4.24KK50 pKa = 10.46QVAQNIAVINAVVMGIVRR68 pKa = 11.84KK69 pKa = 9.88NDD71 pKa = 2.95SRR73 pKa = 4.05

Molecular weight: 8.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

10731

49

1760

188.3

21.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.464 ± 0.464

0.652 ± 0.111

6.206 ± 0.232

6.989 ± 0.447

3.606 ± 0.185

6.71 ± 0.508

1.454 ± 0.162

6.924 ± 0.276

7.697 ± 0.48

7.874 ± 0.276

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.376 ± 0.313

5.433 ± 0.298

3.001 ± 0.263

4.492 ± 0.333

4.426 ± 0.279

6.56 ± 0.395

6.449 ± 0.623

6.924 ± 0.237

1.109 ± 0.086

3.653 ± 0.27
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski