Avon-Heathcote Estuary associated circular virus 7

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 8.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5IAX7|A0A0C5IAX7_9CIRC Putative capsid protein OS=Avon-Heathcote Estuary associated circular virus 7 OX=1618258 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 10.3KK3 pKa = 10.14FKK5 pKa = 10.94EE6 pKa = 4.47KK7 pKa = 9.33ITSLLMRR14 pKa = 11.84FRR16 pKa = 11.84NVVGTLFTNEE26 pKa = 3.99RR27 pKa = 11.84PHH29 pKa = 5.96EE30 pKa = 4.01QFITSPLIKK39 pKa = 10.48YY40 pKa = 9.06FIGQYY45 pKa = 8.71EE46 pKa = 4.12ICPSSKK52 pKa = 9.6RR53 pKa = 11.84RR54 pKa = 11.84HH55 pKa = 4.99LQFYY59 pKa = 10.39CEE61 pKa = 4.38FVGQQRR67 pKa = 11.84LGAVRR72 pKa = 11.84QLFGPTVHH80 pKa = 6.09VEE82 pKa = 4.03PRR84 pKa = 11.84RR85 pKa = 11.84GTVDD89 pKa = 3.19EE90 pKa = 4.36ARR92 pKa = 11.84TYY94 pKa = 10.65CSKK97 pKa = 10.99EE98 pKa = 3.87DD99 pKa = 3.57TRR101 pKa = 11.84EE102 pKa = 3.88PGIDD106 pKa = 3.14SGPFEE111 pKa = 5.14NGTPSKK117 pKa = 10.4SGKK120 pKa = 10.11RR121 pKa = 11.84SDD123 pKa = 3.55LADD126 pKa = 3.28IKK128 pKa = 11.22EE129 pKa = 4.06EE130 pKa = 4.05LDD132 pKa = 3.52AGSSLKK138 pKa = 10.81SISQQHH144 pKa = 6.14FGQFLRR150 pKa = 11.84YY151 pKa = 8.7RR152 pKa = 11.84KK153 pKa = 10.06SFEE156 pKa = 3.78AYY158 pKa = 8.89IVLNQDD164 pKa = 2.48PRR166 pKa = 11.84TWEE169 pKa = 3.96MEE171 pKa = 3.69NSILWGEE178 pKa = 4.2PGTGKK183 pKa = 8.67TKK185 pKa = 10.48LAYY188 pKa = 9.63DD189 pKa = 3.9LKK191 pKa = 10.99EE192 pKa = 4.03RR193 pKa = 11.84DD194 pKa = 3.72GVEE197 pKa = 4.38GYY199 pKa = 10.02PLMRR203 pKa = 11.84NQNGNVWFDD212 pKa = 3.68GYY214 pKa = 10.71HH215 pKa = 4.96GQEE218 pKa = 3.76ILLIDD223 pKa = 5.28DD224 pKa = 3.98YY225 pKa = 11.69YY226 pKa = 11.84GWIPLAFLLQLLDD239 pKa = 4.24RR240 pKa = 11.84YY241 pKa = 9.89PMNVQTKK248 pKa = 8.54GGSVPFTSKK257 pKa = 10.81KK258 pKa = 10.41IIITSNKK265 pKa = 9.87SPEE268 pKa = 3.74CWYY271 pKa = 10.48NWSKK275 pKa = 10.69FGKK278 pKa = 9.96NMFGAFEE285 pKa = 3.88RR286 pKa = 11.84RR287 pKa = 11.84INQVFHH293 pKa = 5.35YY294 pKa = 10.83VKK296 pKa = 10.78DD297 pKa = 3.82KK298 pKa = 11.43DD299 pKa = 3.52PAIYY303 pKa = 9.13PIKK306 pKa = 10.54FPEE309 pKa = 4.26TVSAVEE315 pKa = 4.46FFQQ318 pKa = 5.57

Molecular weight: 36.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5IAX7|A0A0C5IAX7_9CIRC Putative capsid protein OS=Avon-Heathcote Estuary associated circular virus 7 OX=1618258 PE=4 SV=1
MM1 pKa = 6.11VTLMASFRR9 pKa = 11.84FLTFFLYY16 pKa = 10.59SLCQRR21 pKa = 11.84GNAHH25 pKa = 6.75HH26 pKa = 6.84PRR28 pKa = 11.84HH29 pKa = 6.69RR30 pKa = 11.84YY31 pKa = 9.31RR32 pKa = 11.84IIVVMGEE39 pKa = 3.83LTDD42 pKa = 3.47EE43 pKa = 4.29KK44 pKa = 10.8FRR46 pKa = 11.84IGMEE50 pKa = 3.88EE51 pKa = 3.94GLANYY56 pKa = 7.52YY57 pKa = 9.47TPQKK61 pKa = 10.43GAALVRR67 pKa = 11.84AMFPPSHH74 pKa = 5.21TWSWKK79 pKa = 7.56ARR81 pKa = 11.84KK82 pKa = 8.34WFYY85 pKa = 9.36RR86 pKa = 11.84TARR89 pKa = 11.84MRR91 pKa = 11.84MGHH94 pKa = 5.94WLYY97 pKa = 10.66LAAMFLDD104 pKa = 4.93GALTPPQVWLILRR117 pKa = 11.84SRR119 pKa = 11.84AKK121 pKa = 8.5TQQEE125 pKa = 4.18VEE127 pKa = 4.28EE128 pKa = 4.06MTEE131 pKa = 4.03AVKK134 pKa = 10.67KK135 pKa = 10.85LSLGYY140 pKa = 8.36ITKK143 pKa = 9.65IQVWCTMQKK152 pKa = 10.07RR153 pKa = 11.84EE154 pKa = 4.11VPFWMPPAWYY164 pKa = 7.9WKK166 pKa = 10.92NEE168 pKa = 3.71DD169 pKa = 3.63QYY171 pKa = 11.48WFRR174 pKa = 11.84KK175 pKa = 9.72DD176 pKa = 3.39MIWNKK181 pKa = 9.73FYY183 pKa = 11.44

Molecular weight: 22.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

501

183

318

250.5

29.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.79 ± 1.567

1.198 ± 0.057

3.792 ± 0.88

6.587 ± 0.615

6.587 ± 0.316

6.587 ± 1.214

2.196 ± 0.294

5.19 ± 0.748

7.186 ± 0.344

7.585 ± 0.335

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.593 ± 1.625

3.393 ± 0.662

5.389 ± 0.258

4.79 ± 0.23

6.986 ± 0.663

5.389 ± 1.157

5.389 ± 0.341

4.99 ± 0.039

3.393 ± 1.435

4.99 ± 0.26
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski