Okra leaf curl Cameroon virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E5AZV1|E5AZV1_9GEMI C4 protein OS=Okra leaf curl Cameroon virus OX=912034 GN=C4 PE=3 SV=1
MM1 pKa = 7.64APNKK5 pKa = 10.05RR6 pKa = 11.84FQIYY10 pKa = 10.34AKK12 pKa = 10.47NFFLTSPKK20 pKa = 10.15CSLSKK25 pKa = 11.15EE26 pKa = 4.04EE27 pKa = 5.44ALEE30 pKa = 3.78QLQQISTASNKK41 pKa = 10.08KK42 pKa = 9.09YY43 pKa = 10.71IKK45 pKa = 9.68ICRR48 pKa = 11.84EE49 pKa = 3.81LHH51 pKa = 6.97EE52 pKa = 5.88DD53 pKa = 3.95GQPHH57 pKa = 6.66LHH59 pKa = 6.38VLLQFEE65 pKa = 5.47GKK67 pKa = 10.02FKK69 pKa = 10.87CQNQRR74 pKa = 11.84LFDD77 pKa = 4.01LVSPNRR83 pKa = 11.84SAHH86 pKa = 5.06FHH88 pKa = 6.56PNIQGAKK95 pKa = 9.25SSSDD99 pKa = 3.11VKK101 pKa = 11.24SYY103 pKa = 10.79IDD105 pKa = 3.7KK106 pKa = 11.33DD107 pKa = 3.49GDD109 pKa = 3.67TLEE112 pKa = 4.14WGEE115 pKa = 3.84FQIDD119 pKa = 3.21GRR121 pKa = 11.84SARR124 pKa = 11.84GGQQTANDD132 pKa = 4.05AYY134 pKa = 10.69AAALNAGSKK143 pKa = 9.22TEE145 pKa = 3.75ALRR148 pKa = 11.84VIRR151 pKa = 11.84EE152 pKa = 4.09LAPKK156 pKa = 10.51DD157 pKa = 4.02FVLQYY162 pKa = 11.26HH163 pKa = 5.78NLINNLDD170 pKa = 4.39RR171 pKa = 11.84IFQEE175 pKa = 4.19PPAPYY180 pKa = 9.75VCPFLSSSFDD190 pKa = 3.44QVPEE194 pKa = 3.98EE195 pKa = 4.13LEE197 pKa = 3.58EE198 pKa = 3.9WAAEE202 pKa = 4.15NVVEE206 pKa = 4.32AAARR210 pKa = 11.84PGRR213 pKa = 11.84PISIVIEE220 pKa = 4.35GEE222 pKa = 3.93SRR224 pKa = 11.84TGKK227 pKa = 7.7TVWARR232 pKa = 11.84SLGPHH237 pKa = 6.71NYY239 pKa = 10.18LCGHH243 pKa = 7.38LDD245 pKa = 3.88LSPKK249 pKa = 10.04VFSNDD254 pKa = 1.85AWYY257 pKa = 10.93NVIDD261 pKa = 5.2DD262 pKa = 4.41VDD264 pKa = 3.61PHH266 pKa = 5.85YY267 pKa = 11.0LKK269 pKa = 10.7HH270 pKa = 6.2FKK272 pKa = 10.76EE273 pKa = 4.49FMGAQKK279 pKa = 10.58DD280 pKa = 3.71WQSNTKK286 pKa = 9.54YY287 pKa = 10.57GKK289 pKa = 9.12PVQIKK294 pKa = 10.41GGIPTIFLCNPGPNSSYY311 pKa = 11.0KK312 pKa = 10.63DD313 pKa = 3.51YY314 pKa = 11.67LEE316 pKa = 4.37EE317 pKa = 4.39EE318 pKa = 4.57KK319 pKa = 10.84NAHH322 pKa = 5.75LKK324 pKa = 9.97SWALKK329 pKa = 8.81NATFITLSYY338 pKa = 10.0PLYY341 pKa = 10.72SGTNQSPASGGQEE354 pKa = 3.63EE355 pKa = 5.28SNQEE359 pKa = 3.99TQDD362 pKa = 3.25

Molecular weight: 40.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E5AZU8|E5AZU8_9GEMI Replication enhancer OS=Okra leaf curl Cameroon virus OX=912034 GN=REn PE=3 SV=1
MM1 pKa = 7.7SKK3 pKa = 10.44RR4 pKa = 11.84PGDD7 pKa = 3.95IIISTPVSKK16 pKa = 10.56VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.04SPYY27 pKa = 8.58TSRR30 pKa = 11.84AAVPTVLGTSKK41 pKa = 10.22RR42 pKa = 11.84RR43 pKa = 11.84SWTYY47 pKa = 9.79RR48 pKa = 11.84PMYY51 pKa = 9.54RR52 pKa = 11.84KK53 pKa = 8.92PRR55 pKa = 11.84MYY57 pKa = 10.84RR58 pKa = 11.84MYY60 pKa = 10.6KK61 pKa = 10.21SPDD64 pKa = 3.18VPRR67 pKa = 11.84GCEE70 pKa = 4.4GPCKK74 pKa = 9.97IQSFDD79 pKa = 3.29QRR81 pKa = 11.84DD82 pKa = 4.15SVTHH86 pKa = 6.34TGNSRR91 pKa = 11.84CLSDD95 pKa = 3.29VTRR98 pKa = 11.84GNGLTHH104 pKa = 6.08RR105 pKa = 11.84TGKK108 pKa = 10.3RR109 pKa = 11.84FVIKK113 pKa = 10.19SVYY116 pKa = 10.04ILGKK120 pKa = 9.4VWMDD124 pKa = 3.43EE125 pKa = 3.95NVKK128 pKa = 10.46KK129 pKa = 10.93SNHH132 pKa = 5.83TNTCMFWLVRR142 pKa = 11.84DD143 pKa = 3.64RR144 pKa = 11.84RR145 pKa = 11.84PYY147 pKa = 10.9GNSPQDD153 pKa = 3.31FGQVFNMFDD162 pKa = 3.99NEE164 pKa = 4.12PSTATVMNDD173 pKa = 2.37KK174 pKa = 10.42RR175 pKa = 11.84DD176 pKa = 3.47RR177 pKa = 11.84YY178 pKa = 9.73QVLKK182 pKa = 10.6RR183 pKa = 11.84FQATVTGGPSGCKK196 pKa = 9.03EE197 pKa = 3.84SAIVRR202 pKa = 11.84RR203 pKa = 11.84FFRR206 pKa = 11.84INHH209 pKa = 5.65HH210 pKa = 4.7VTYY213 pKa = 10.55NHH215 pKa = 6.09QEE217 pKa = 3.49AAKK220 pKa = 10.4YY221 pKa = 9.25EE222 pKa = 4.13NHH224 pKa = 6.46TEE226 pKa = 3.99NALLLYY232 pKa = 7.29MACTHH237 pKa = 7.07ASNPVYY243 pKa = 10.67ASLKK247 pKa = 8.7IRR249 pKa = 11.84MYY251 pKa = 10.75FYY253 pKa = 11.17DD254 pKa = 3.64SVSNN258 pKa = 3.9

Molecular weight: 29.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1129

97

362

188.2

21.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.403 ± 0.912

2.303 ± 0.313

4.783 ± 0.683

5.137 ± 0.871

4.074 ± 0.366

5.226 ± 0.483

3.277 ± 0.257

5.403 ± 0.777

5.58 ± 0.695

7.352 ± 1.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.391 ± 0.708

6.377 ± 0.458

5.757 ± 0.436

5.314 ± 0.754

7.174 ± 1.412

8.06 ± 0.808

6.023 ± 1.051

5.137 ± 0.926

1.329 ± 0.14

3.897 ± 0.608
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski