Caloramator fervidus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Caloramator

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1775 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H5V4X9|A0A1H5V4X9_9CLOT Exodeoxyribonuclease 7 small subunit OS=Caloramator fervidus OX=29344 GN=xseB PE=3 SV=1
MM1 pKa = 7.48NIVKK5 pKa = 10.01EE6 pKa = 4.08RR7 pKa = 11.84VSYY10 pKa = 11.0LKK12 pKa = 11.01GLADD16 pKa = 3.42GLGINEE22 pKa = 4.18DD23 pKa = 3.66TKK25 pKa = 11.07EE26 pKa = 4.1GKK28 pKa = 10.36LLIAIIDD35 pKa = 3.86VLDD38 pKa = 5.32EE39 pKa = 3.9IAEE42 pKa = 4.49EE43 pKa = 3.81IDD45 pKa = 3.96RR46 pKa = 11.84IYY48 pKa = 11.17GEE50 pKa = 4.01QADD53 pKa = 4.06LEE55 pKa = 4.63EE56 pKa = 4.61YY57 pKa = 10.99VDD59 pKa = 6.38AIDD62 pKa = 4.34EE63 pKa = 4.44DD64 pKa = 4.16LTRR67 pKa = 11.84LEE69 pKa = 4.64DD70 pKa = 3.97EE71 pKa = 5.21LYY73 pKa = 10.96EE74 pKa = 6.08DD75 pKa = 5.95DD76 pKa = 6.26DD77 pKa = 5.11YY78 pKa = 12.14EE79 pKa = 6.52DD80 pKa = 3.89DD81 pKa = 4.2AFVEE85 pKa = 4.92VEE87 pKa = 4.54CPHH90 pKa = 6.73CNEE93 pKa = 5.42KK94 pKa = 10.49IFLDD98 pKa = 3.65KK99 pKa = 10.97EE100 pKa = 4.22VFEE103 pKa = 5.29DD104 pKa = 4.1EE105 pKa = 4.28EE106 pKa = 4.65EE107 pKa = 4.28EE108 pKa = 5.3EE109 pKa = 4.87IICPNCNEE117 pKa = 4.5PIFIKK122 pKa = 10.04EE123 pKa = 3.8EE124 pKa = 4.32CEE126 pKa = 4.93CDD128 pKa = 5.5SEE130 pKa = 5.41DD131 pKa = 4.71CNCC134 pKa = 4.79

Molecular weight: 15.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H5WFJ5|A0A1H5WFJ5_9CLOT Beta sliding clamp OS=Caloramator fervidus OX=29344 GN=SAMN05660865_01454 PE=3 SV=1
MM1 pKa = 7.45LRR3 pKa = 11.84TYY5 pKa = 9.82QPKK8 pKa = 9.43KK9 pKa = 8.02RR10 pKa = 11.84HH11 pKa = 5.67RR12 pKa = 11.84KK13 pKa = 8.75KK14 pKa = 9.79EE15 pKa = 3.46HH16 pKa = 5.76GFRR19 pKa = 11.84KK20 pKa = 9.99RR21 pKa = 11.84MSTRR25 pKa = 11.84NGRR28 pKa = 11.84KK29 pKa = 7.46VLKK32 pKa = 9.55RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84MKK37 pKa = 10.3GRR39 pKa = 11.84KK40 pKa = 9.06RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1775

0

1775

538899

41

2821

303.6

34.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.855 ± 0.054

1.091 ± 0.023

5.487 ± 0.048

7.511 ± 0.066

4.734 ± 0.053

6.432 ± 0.057

1.282 ± 0.024

10.36 ± 0.074

9.682 ± 0.057

9.516 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.385 ± 0.023

5.537 ± 0.053

3.079 ± 0.036

2.262 ± 0.024

3.672 ± 0.045

5.222 ± 0.048

4.256 ± 0.034

6.965 ± 0.053

0.554 ± 0.019

4.115 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski