Paracoccus phage vB_PsuS_Psul1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 7.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3T0IK20|A0A3T0IK20_9CAUD Scaffolding protein OS=Paracoccus phage vB_PsuS_Psul1 OX=2500569 GN=psul1_p31 PE=4 SV=1
MM1 pKa = 7.43SDD3 pKa = 2.88KK4 pKa = 10.28VTVYY8 pKa = 10.04EE9 pKa = 4.18AATGKK14 pKa = 9.53IRR16 pKa = 11.84RR17 pKa = 11.84VLLLPLVGMLDD28 pKa = 3.63EE29 pKa = 5.55DD30 pKa = 4.65GEE32 pKa = 4.73IVGHH36 pKa = 7.09IINPVDD42 pKa = 3.82LANNVAGDD50 pKa = 3.56EE51 pKa = 4.26RR52 pKa = 11.84YY53 pKa = 10.41VLGEE57 pKa = 3.89FGSDD61 pKa = 2.48RR62 pKa = 11.84FYY64 pKa = 11.05WDD66 pKa = 3.61GSAMAAFPDD75 pKa = 4.79PPSEE79 pKa = 3.67WAEE82 pKa = 3.6FDD84 pKa = 3.34FAARR88 pKa = 11.84QWVDD92 pKa = 2.83HH93 pKa = 6.5YY94 pKa = 11.89DD95 pKa = 3.59PALALAAARR104 pKa = 11.84ASANMTRR111 pKa = 11.84SDD113 pKa = 3.92FLLAAISAGIILPSDD128 pKa = 4.09AGPAARR134 pKa = 11.84GEE136 pKa = 4.31IPPSLVPVFDD146 pKa = 4.15GLPLEE151 pKa = 4.32VRR153 pKa = 11.84IEE155 pKa = 3.97AVVRR159 pKa = 11.84WGAATVIEE167 pKa = 4.34RR168 pKa = 11.84TNPVLAALAEE178 pKa = 4.2AMAITDD184 pKa = 4.08ADD186 pKa = 4.04LDD188 pKa = 4.01ALFGISS194 pKa = 3.49

Molecular weight: 20.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q9R8W0|A0A3Q9R8W0_9CAUD Putative replication protein OS=Paracoccus phage vB_PsuS_Psul1 OX=2500569 GN=psul1_p21 PE=4 SV=1
MM1 pKa = 7.18TKK3 pKa = 10.6AFRR6 pKa = 11.84EE7 pKa = 3.96AAAARR12 pKa = 11.84IAAEE16 pKa = 4.24RR17 pKa = 11.84ARR19 pKa = 11.84LDD21 pKa = 4.52AILHH25 pKa = 6.2RR26 pKa = 11.84ATPVDD31 pKa = 3.33GCGPAIPIAPARR43 pKa = 11.84GPQIVVTPRR52 pKa = 11.84RR53 pKa = 11.84MVPDD57 pKa = 3.96AKK59 pKa = 10.75DD60 pKa = 3.17ATGWKK65 pKa = 9.88VEE67 pKa = 3.97EE68 pKa = 4.37LGWRR72 pKa = 11.84GFNAVRR78 pKa = 11.84ATDD81 pKa = 3.36IFDD84 pKa = 4.12DD85 pKa = 4.43LARR88 pKa = 11.84RR89 pKa = 11.84AAKK92 pKa = 10.34RR93 pKa = 11.84KK94 pKa = 9.02DD95 pKa = 3.56APPFTPGQVAVGRR108 pKa = 11.84LYY110 pKa = 10.27RR111 pKa = 11.84DD112 pKa = 2.95LVEE115 pKa = 4.22RR116 pKa = 11.84HH117 pKa = 6.43DD118 pKa = 4.26AGGMRR123 pKa = 11.84CASLEE128 pKa = 3.94AGRR131 pKa = 11.84GGSPSGGGEE140 pKa = 4.05FIDD143 pKa = 4.5AFIEE147 pKa = 3.93EE148 pKa = 4.36GRR150 pKa = 11.84VIEE153 pKa = 4.01LLRR156 pKa = 11.84RR157 pKa = 11.84RR158 pKa = 11.84IGHH161 pKa = 5.9GVAMPVRR168 pKa = 11.84RR169 pKa = 11.84VRR171 pKa = 11.84PSARR175 pKa = 11.84GGDD178 pKa = 4.27GARR181 pKa = 11.84MIHH184 pKa = 6.93DD185 pKa = 3.89RR186 pKa = 11.84VLVDD190 pKa = 5.73DD191 pKa = 4.2ICLHH195 pKa = 5.65GRR197 pKa = 11.84SFQSVLEE204 pKa = 3.95RR205 pKa = 11.84HH206 pKa = 5.85GWTKK210 pKa = 9.95TGRR213 pKa = 11.84NVVALQCALAACLDD227 pKa = 3.53RR228 pKa = 11.84MQFHH232 pKa = 7.13SKK234 pKa = 9.5MGLTTT239 pKa = 3.72

Molecular weight: 26.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

10707

41

1143

187.8

20.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.795 ± 0.498

0.897 ± 0.158

5.875 ± 0.241

6.024 ± 0.427

3.269 ± 0.17

8.396 ± 0.33

2.008 ± 0.216

5.053 ± 0.226

3.699 ± 0.255

8.499 ± 0.307

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.933 ± 0.157

2.568 ± 0.184

5.286 ± 0.338

3.456 ± 0.18

8.154 ± 0.495

5.912 ± 0.24

5.333 ± 0.317

6.005 ± 0.317

1.933 ± 0.149

1.905 ± 0.186
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski