Streptococcus phage phiD12

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1Q209|M1Q209_9CAUD Isoform of M1PSC0 Uncharacterized protein OS=Streptococcus phage phiD12 OX=1289600 GN=phiD12_0033 PE=4 SV=1
MM1 pKa = 7.36WEE3 pKa = 3.55IMTRR7 pKa = 11.84TVGDD11 pKa = 3.18RR12 pKa = 11.84HH13 pKa = 6.33YY14 pKa = 10.33ICEE17 pKa = 4.19FLRR20 pKa = 11.84EE21 pKa = 4.26DD22 pKa = 3.41TTDD25 pKa = 3.05PRR27 pKa = 11.84NIDD30 pKa = 3.61GAWIRR35 pKa = 11.84ILTIKK40 pKa = 10.43RR41 pKa = 11.84DD42 pKa = 3.31GEE44 pKa = 4.16YY45 pKa = 9.72IYY47 pKa = 10.36QYY49 pKa = 10.86RR50 pKa = 11.84YY51 pKa = 10.77GNEE54 pKa = 3.93IDD56 pKa = 4.6NMDD59 pKa = 5.1DD60 pKa = 3.19IDD62 pKa = 3.95RR63 pKa = 11.84TVCQAVLDD71 pKa = 4.16NFNEE75 pKa = 4.18LL76 pKa = 3.58

Molecular weight: 9.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1PSD2|M1PSD2_9CAUD Isoform of M1NSK8 Uncharacterized protein OS=Streptococcus phage phiD12 OX=1289600 GN=phiD12_0016 PE=4 SV=1
MM1 pKa = 7.73PRR3 pKa = 11.84RR4 pKa = 11.84PSIPCKK10 pKa = 10.5QNGCPNLVSYY20 pKa = 7.88GQKK23 pKa = 9.68YY24 pKa = 9.61CEE26 pKa = 3.77NHH28 pKa = 6.52KK29 pKa = 10.98ANHH32 pKa = 5.69QLDD35 pKa = 4.16SKK37 pKa = 8.65STKK40 pKa = 10.13AKK42 pKa = 10.34GYY44 pKa = 8.38NAQWNKK50 pKa = 10.15ARR52 pKa = 11.84LRR54 pKa = 11.84YY55 pKa = 9.99LKK57 pKa = 9.81VHH59 pKa = 6.46PLCVQCKK66 pKa = 9.75AKK68 pKa = 10.52GRR70 pKa = 11.84LTKK73 pKa = 10.1ATVVDD78 pKa = 5.28HH79 pKa = 6.23ITPHH83 pKa = 6.97RR84 pKa = 11.84GDD86 pKa = 5.06QEE88 pKa = 5.0LFWNQTNWQALCKK101 pKa = 9.93SCHH104 pKa = 5.64DD105 pKa = 4.22RR106 pKa = 11.84KK107 pKa = 8.93TKK109 pKa = 8.13TTDD112 pKa = 2.64RR113 pKa = 11.84YY114 pKa = 10.3VEE116 pKa = 3.73YY117 pKa = 10.38SYY119 pKa = 11.5RR120 pKa = 11.84FF121 pKa = 3.35

Molecular weight: 14.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24

31

55

15127

53

1515

275.0

30.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.014 ± 0.391

0.721 ± 0.108

5.705 ± 0.268

7.146 ± 0.341

3.814 ± 0.175

6.597 ± 0.328

1.977 ± 0.151

6.948 ± 0.273

7.166 ± 0.257

9.308 ± 0.388

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.261 ± 0.183

4.76 ± 0.231

2.948 ± 0.173

4.76 ± 0.228

4.641 ± 0.192

6.386 ± 0.375

6.591 ± 0.44

6.373 ± 0.252

1.249 ± 0.111

3.636 ± 0.253
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski