Paenibacillus sp. P1XP2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6608 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B0I601|A0A0B0I601_9BACL Putative 6-phospho-beta-glucosidase OS=Paenibacillus sp. P1XP2 OX=1472719 GN=licH PE=3 SV=1
MM1 pKa = 7.71SDD3 pKa = 3.12HH4 pKa = 5.35THH6 pKa = 5.36EE7 pKa = 5.48HH8 pKa = 6.73GDD10 pKa = 3.66ACGHH14 pKa = 6.36DD15 pKa = 3.76HH16 pKa = 7.55DD17 pKa = 5.72HH18 pKa = 6.71DD19 pKa = 4.65HH20 pKa = 5.86EE21 pKa = 5.79HH22 pKa = 6.82EE23 pKa = 4.33EE24 pKa = 4.46FVLTLTDD31 pKa = 3.45EE32 pKa = 4.22QGNDD36 pKa = 3.01VEE38 pKa = 4.55MVLVEE43 pKa = 4.14TFDD46 pKa = 3.63VGEE49 pKa = 4.1NLYY52 pKa = 11.19ALLLEE57 pKa = 4.69RR58 pKa = 11.84EE59 pKa = 4.2NPEE62 pKa = 3.48ADD64 pKa = 4.07GIILRR69 pKa = 11.84VEE71 pKa = 4.16EE72 pKa = 4.76EE73 pKa = 4.05NDD75 pKa = 3.23EE76 pKa = 4.05MMLYY80 pKa = 10.43NIEE83 pKa = 6.06DD84 pKa = 3.8EE85 pKa = 4.72EE86 pKa = 4.11EE87 pKa = 4.11WNAVEE92 pKa = 4.06AAYY95 pKa = 10.96NEE97 pKa = 4.52LVAQQEE103 pKa = 4.49DD104 pKa = 3.63

Molecular weight: 12.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B0IAQ8|A0A0B0IAQ8_9BACL Uncharacterized protein OS=Paenibacillus sp. P1XP2 OX=1472719 GN=CM49_01112 PE=4 SV=1
MM1 pKa = 7.45NGGIIGGILGGIGAGLLVQFLFVKK25 pKa = 9.9CVQWRR30 pKa = 11.84FPMTTVNIAAGGFAGLVSGLIMYY53 pKa = 8.41YY54 pKa = 10.3LVAPFALQAGEE65 pKa = 4.13FIKK68 pKa = 11.05YY69 pKa = 10.17LINLAISFNPIVAGVIGGLLIWPAILGGVYY99 pKa = 9.83HH100 pKa = 7.16AAILPIVLLEE110 pKa = 4.01MEE112 pKa = 4.22KK113 pKa = 9.99TGNSFLGAIDD123 pKa = 3.58MVGLVMVSAGITLANLVAPRR143 pKa = 11.84DD144 pKa = 3.7KK145 pKa = 11.64GEE147 pKa = 4.14AAVAAPGFLINMGFGTFVEE166 pKa = 4.82AATLSCFRR174 pKa = 11.84ISGYY178 pKa = 9.96SGEE181 pKa = 4.62RR182 pKa = 11.84LRR184 pKa = 11.84QPGSAAVWSACFKK197 pKa = 10.42SAARR201 pKa = 11.84PTSRR205 pKa = 11.84RR206 pKa = 11.84SWGLSCPITWWDD218 pKa = 3.65LRR220 pKa = 11.84SPWRR224 pKa = 11.84LRR226 pKa = 11.84CCSRR230 pKa = 11.84LRR232 pKa = 11.84LRR234 pKa = 11.84WWRR237 pKa = 11.84TKK239 pKa = 10.74RR240 pKa = 11.84PGDD243 pKa = 3.21AKK245 pKa = 10.6RR246 pKa = 11.84RR247 pKa = 11.84IEE249 pKa = 4.14TCPPSNPSPKK259 pKa = 10.1RR260 pKa = 11.84KK261 pKa = 10.18DD262 pKa = 3.17PGRR265 pKa = 11.84LGKK268 pKa = 10.42GRR270 pKa = 11.84SGLFFISTRR279 pKa = 11.84SGFSGISSLNRR290 pKa = 11.84PGFCRR295 pKa = 11.84EE296 pKa = 4.07PGSPASDD303 pKa = 3.64PFYY306 pKa = 10.9ASSPEE311 pKa = 4.12VIRR314 pKa = 11.84TQRR317 pKa = 11.84NPLL320 pKa = 3.43

Molecular weight: 34.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6608

0

6608

1641588

29

1621

248.4

27.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.617 ± 0.039

0.861 ± 0.01

5.078 ± 0.025

6.718 ± 0.038

4.143 ± 0.023

7.666 ± 0.032

2.096 ± 0.016

6.304 ± 0.034

5.598 ± 0.032

9.862 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.986 ± 0.017

3.541 ± 0.022

4.319 ± 0.02

3.672 ± 0.02

5.589 ± 0.031

6.177 ± 0.025

5.132 ± 0.021

6.932 ± 0.027

1.337 ± 0.015

3.372 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski