Cotton leaf curl Gezira virus-[okra:BFA]

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; Cotton leaf curl Gezira virus

Average proteome isoelectric point is 7.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D1GZH2|D1GZH2_9GEMI Replication-associated protein OS=Cotton leaf curl Gezira virus-[okra:BFA] OX=679314 GN=C1 PE=3 SV=1
MM1 pKa = 7.53PPSKK5 pKa = 10.45RR6 pKa = 11.84FLINAKK12 pKa = 10.25NYY14 pKa = 9.48FLTFPKK20 pKa = 10.48CSLTKK25 pKa = 10.73EE26 pKa = 3.96EE27 pKa = 5.5ALEE30 pKa = 3.8QLQKK34 pKa = 10.81ISTASNKK41 pKa = 10.04KK42 pKa = 9.09YY43 pKa = 10.71IKK45 pKa = 9.68ICRR48 pKa = 11.84EE49 pKa = 3.81LHH51 pKa = 6.97EE52 pKa = 5.88DD53 pKa = 3.95GQPHH57 pKa = 6.66LHH59 pKa = 6.38VLLQFEE65 pKa = 5.47GKK67 pKa = 10.02FKK69 pKa = 10.71CQNHH73 pKa = 6.27RR74 pKa = 11.84LFDD77 pKa = 4.0LVSPNRR83 pKa = 11.84SAHH86 pKa = 5.06FHH88 pKa = 6.56PNIQGAKK95 pKa = 9.25SSSDD99 pKa = 3.11VKK101 pKa = 11.24SYY103 pKa = 10.79IDD105 pKa = 3.7KK106 pKa = 11.33DD107 pKa = 3.49GDD109 pKa = 3.67TLEE112 pKa = 4.14WGEE115 pKa = 3.84FQIDD119 pKa = 3.21GRR121 pKa = 11.84SARR124 pKa = 11.84GGQQTANDD132 pKa = 4.05AYY134 pKa = 10.69AAALNAGSKK143 pKa = 9.22TEE145 pKa = 3.75ALRR148 pKa = 11.84VIRR151 pKa = 11.84EE152 pKa = 4.09LAPKK156 pKa = 10.52DD157 pKa = 3.64FVLQFHH163 pKa = 6.68NLNSNLEE170 pKa = 4.41RR171 pKa = 11.84IFQEE175 pKa = 4.14PPAPYY180 pKa = 9.99VSPFLSSSFDD190 pKa = 3.44QVPEE194 pKa = 3.98EE195 pKa = 4.13LEE197 pKa = 3.58EE198 pKa = 3.9WAAEE202 pKa = 4.15NVVEE206 pKa = 4.74AAARR210 pKa = 11.84PDD212 pKa = 3.51RR213 pKa = 11.84PISIVIEE220 pKa = 4.35GEE222 pKa = 3.93SRR224 pKa = 11.84TGKK227 pKa = 7.7TVWARR232 pKa = 11.84SLGPHH237 pKa = 6.71NYY239 pKa = 10.18LCGHH243 pKa = 7.38LDD245 pKa = 3.88LSPKK249 pKa = 10.04VFSNDD254 pKa = 1.85AWYY257 pKa = 10.93NVIDD261 pKa = 5.2DD262 pKa = 4.41VDD264 pKa = 3.61PHH266 pKa = 5.85YY267 pKa = 11.0LKK269 pKa = 10.7HH270 pKa = 6.2FKK272 pKa = 10.76EE273 pKa = 4.49FMGAQKK279 pKa = 10.58DD280 pKa = 3.71WQSNTKK286 pKa = 9.37YY287 pKa = 10.59GKK289 pKa = 8.89PVKK292 pKa = 9.95IKK294 pKa = 10.84GGIPTIFLCNPGPNSSYY311 pKa = 11.07KK312 pKa = 10.49EE313 pKa = 3.93YY314 pKa = 11.01LDD316 pKa = 3.86EE317 pKa = 4.99EE318 pKa = 4.77KK319 pKa = 10.73NAHH322 pKa = 5.77LKK324 pKa = 9.97SWALKK329 pKa = 8.81NATFITLSNPLYY341 pKa = 10.77SGTNQSPASGGEE353 pKa = 3.96EE354 pKa = 3.9EE355 pKa = 5.46SNQEE359 pKa = 4.0TQDD362 pKa = 3.25

Molecular weight: 40.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D1GZ44|D1GZ44_9GEMI Replication enhancer OS=Cotton leaf curl Gezira virus-[okra:BFA] OX=679314 GN=C3 PE=3 SV=1
MM1 pKa = 7.71SKK3 pKa = 10.35RR4 pKa = 11.84PADD7 pKa = 3.87IIISTPASKK16 pKa = 10.3VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.39SPGLSSARR32 pKa = 11.84APTVLVTNKK41 pKa = 9.08RR42 pKa = 11.84RR43 pKa = 11.84AWTNRR48 pKa = 11.84PTYY51 pKa = 9.74RR52 pKa = 11.84KK53 pKa = 8.33PRR55 pKa = 11.84MYY57 pKa = 10.88RR58 pKa = 11.84MYY60 pKa = 10.57RR61 pKa = 11.84SPDD64 pKa = 3.29VPKK67 pKa = 10.81GCEE70 pKa = 4.39GPCKK74 pKa = 9.35VQSYY78 pKa = 6.99EE79 pKa = 3.67QRR81 pKa = 11.84DD82 pKa = 4.05DD83 pKa = 3.72VKK85 pKa = 9.5HH86 pKa = 5.48TGIVRR91 pKa = 11.84CVSDD95 pKa = 3.35VTKK98 pKa = 10.89GVGLTHH104 pKa = 6.27RR105 pKa = 11.84TGKK108 pKa = 10.36RR109 pKa = 11.84FTIKK113 pKa = 10.25SIYY116 pKa = 9.93ILGKK120 pKa = 9.79VWMDD124 pKa = 4.63DD125 pKa = 3.63NIKK128 pKa = 10.18KK129 pKa = 10.0QNHH132 pKa = 5.11TNNVMFFLVRR142 pKa = 11.84DD143 pKa = 3.63RR144 pKa = 11.84RR145 pKa = 11.84PYY147 pKa = 10.95GNSPLDD153 pKa = 3.58FGQVFNMFDD162 pKa = 3.99NEE164 pKa = 4.11PSTATVKK171 pKa = 10.87NDD173 pKa = 3.36LRR175 pKa = 11.84DD176 pKa = 3.45HH177 pKa = 5.8FQVLRR182 pKa = 11.84KK183 pKa = 8.36FTATVIGGPSGMKK196 pKa = 9.37EE197 pKa = 3.6QALVRR202 pKa = 11.84RR203 pKa = 11.84FFRR206 pKa = 11.84INSQIVYY213 pKa = 9.33NHH215 pKa = 5.55QEE217 pKa = 3.51AGKK220 pKa = 10.45FEE222 pKa = 4.2NHH224 pKa = 5.77TEE226 pKa = 3.95NAILLYY232 pKa = 8.57MACTHH237 pKa = 7.07ASNPVYY243 pKa = 9.86ATLKK247 pKa = 9.47IRR249 pKa = 11.84IYY251 pKa = 10.67FYY253 pKa = 11.26DD254 pKa = 3.51SVSNN258 pKa = 3.9

Molecular weight: 29.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1109

100

362

184.8

21.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.05 ± 0.984

1.894 ± 0.51

4.959 ± 0.736

5.5 ± 0.904

4.418 ± 0.536

5.23 ± 0.274

3.517 ± 0.443

5.41 ± 0.967

5.771 ± 1.007

7.484 ± 0.877

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.164 ± 0.649

6.041 ± 0.727

5.591 ± 0.599

5.23 ± 0.832

7.214 ± 1.311

8.206 ± 1.158

5.951 ± 0.953

5.681 ± 0.97

1.262 ± 0.176

3.427 ± 0.406
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski