bacterium E08(2017)

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1267 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A202DKA1|A0A202DKA1_9BACT Uncharacterized protein OS=bacterium E08(2017) OX=1932693 GN=BVX97_01230 PE=4 SV=1
MM1 pKa = 7.68SFRR4 pKa = 11.84KK5 pKa = 10.12LSPKK9 pKa = 9.57LRR11 pKa = 11.84LMIVSFVVMSFVFVGIALDD30 pKa = 3.68TDD32 pKa = 3.73KK33 pKa = 11.74DD34 pKa = 3.89GMSDD38 pKa = 3.36NFEE41 pKa = 4.88LLFDD45 pKa = 5.37LNPNNQEE52 pKa = 4.29DD53 pKa = 3.88ADD55 pKa = 4.3LNYY58 pKa = 11.18DD59 pKa = 3.47SDD61 pKa = 3.99NLINSEE67 pKa = 4.29EE68 pKa = 4.26YY69 pKa = 11.4ANATDD74 pKa = 3.44PWEE77 pKa = 4.32TDD79 pKa = 2.65TDD81 pKa = 3.99RR82 pKa = 11.84DD83 pKa = 3.67GWSDD87 pKa = 4.81DD88 pKa = 3.37VDD90 pKa = 3.8GDD92 pKa = 3.64PTSRR96 pKa = 11.84LFIDD100 pKa = 3.42WGEE103 pKa = 3.95PLYY106 pKa = 9.94TFCDD110 pKa = 4.09DD111 pKa = 3.72YY112 pKa = 11.81VYY114 pKa = 10.55TGPLWWMSAFKK125 pKa = 10.94LGGEE129 pKa = 4.34WVATTNFTSWYY140 pKa = 9.11SGNASDD146 pKa = 5.42RR147 pKa = 11.84VMIEE151 pKa = 4.15LDD153 pKa = 3.26RR154 pKa = 11.84SLITQPVVLDD164 pKa = 3.38MDD166 pKa = 5.59LYY168 pKa = 9.73SQNGSAVSVDD178 pKa = 4.24LLDD181 pKa = 5.46ANDD184 pKa = 3.96SVVASDD190 pKa = 3.84IFGNTIAVPAAKK202 pKa = 9.84SVSRR206 pKa = 11.84SVDD209 pKa = 2.81INLPLYY215 pKa = 10.85SDD217 pKa = 4.03AASIAIGPAFGDD229 pKa = 3.33LSIFQTHH236 pKa = 5.87VYY238 pKa = 10.2VDD240 pKa = 3.67QDD242 pKa = 3.25HH243 pKa = 7.63DD244 pKa = 3.98GLDD247 pKa = 3.88AEE249 pKa = 5.17QEE251 pKa = 4.3AQLGTSDD258 pKa = 5.42LSADD262 pKa = 3.58SDD264 pKa = 4.21GDD266 pKa = 3.71GMSDD270 pKa = 3.66YY271 pKa = 11.62EE272 pKa = 4.16EE273 pKa = 4.15VFYY276 pKa = 10.84IGSDD280 pKa = 3.49PSSAPMIAPVVAGGGAGDD298 pKa = 3.88PEE300 pKa = 5.59GYY302 pKa = 10.62ASLIATQTVAGSQDD316 pKa = 2.91INLASVTALDD326 pKa = 3.9WVGSSVDD333 pKa = 3.45ANKK336 pKa = 8.78FTVGANGVITVTDD349 pKa = 3.59AGDD352 pKa = 3.65YY353 pKa = 9.51MIAAALPMYY362 pKa = 10.46SAITRR367 pKa = 11.84SCVRR371 pKa = 11.84LQAYY375 pKa = 10.26VNGTVVPGLVGEE387 pKa = 4.29SSYY390 pKa = 11.23VRR392 pKa = 11.84NTSGHH397 pKa = 5.87NEE399 pKa = 4.25SSCHH403 pKa = 5.67FAGLLKK409 pKa = 10.61GLNAGDD415 pKa = 3.74QIEE418 pKa = 4.5VKK420 pKa = 10.86VLGTAVAGSGVSMSTARR437 pKa = 11.84LYY439 pKa = 10.44MEE441 pKa = 6.27AIDD444 pKa = 4.05ASRR447 pKa = 11.84VVYY450 pKa = 10.6SGLSDD455 pKa = 3.84GPAGGDD461 pKa = 3.45VNPATAAPMLWTTDD475 pKa = 3.24QRR477 pKa = 11.84IDD479 pKa = 3.57SGFTHH484 pKa = 6.66SAGSQSITLDD494 pKa = 3.47DD495 pKa = 4.18GGVYY499 pKa = 10.47LVYY502 pKa = 10.79FNMPVYY508 pKa = 9.96STVSRR513 pKa = 11.84SAPRR517 pKa = 11.84IEE519 pKa = 3.96MKK521 pKa = 10.14IDD523 pKa = 3.0GVMVAGGAGKK533 pKa = 9.86QGYY536 pKa = 9.1IKK538 pKa = 10.71GGSHH542 pKa = 7.0SDD544 pKa = 3.89ASVHH548 pKa = 4.64WSGVIQASAGSVLTVDD564 pKa = 4.31TMQEE568 pKa = 3.45AAAGVVTVPAGEE580 pKa = 4.16KK581 pKa = 10.82AMLTIEE587 pKa = 4.81KK588 pKa = 9.95LDD590 pKa = 3.87ASQDD594 pKa = 3.57VYY596 pKa = 11.25FGLGISLDD604 pKa = 4.33SGDD607 pKa = 3.68NWNVAGSVQFADD619 pKa = 4.47QIIDD623 pKa = 3.39GTKK626 pKa = 7.92YY627 pKa = 10.65SKK629 pKa = 10.39PATHH633 pKa = 5.88QVQVSEE639 pKa = 4.38AGDD642 pKa = 3.58YY643 pKa = 11.3LLVYY647 pKa = 10.35NDD649 pKa = 3.7SFYY652 pKa = 11.28ATLSRR657 pKa = 11.84ANPKK661 pKa = 8.73VTVTVNGAAVPGAEE675 pKa = 4.39VKK677 pKa = 10.18SHH679 pKa = 7.3HH680 pKa = 6.46IRR682 pKa = 11.84NSSGHH687 pKa = 5.67NEE689 pKa = 4.05SSGSLVTMLDD699 pKa = 3.43GLSAGDD705 pKa = 3.56VVAIALEE712 pKa = 4.13QEE714 pKa = 4.2AAGGVVDD721 pKa = 5.61DD722 pKa = 5.45DD723 pKa = 4.53APAVLALISKK733 pKa = 10.54AGGQVGPQAPVANAGADD750 pKa = 3.62QNVEE754 pKa = 4.1DD755 pKa = 4.36TDD757 pKa = 4.5GNGSEE762 pKa = 4.64SVSLDD767 pKa = 3.12GSGSTDD773 pKa = 2.92SDD775 pKa = 3.75GTIVSYY781 pKa = 10.94SWTTGATEE789 pKa = 3.86IATGVAPVVSLPVGANVVTLTVEE812 pKa = 4.48DD813 pKa = 4.61DD814 pKa = 4.37GGLQDD819 pKa = 4.15SDD821 pKa = 4.01TVVITVNPAPANEE834 pKa = 4.25APVASFTAAPGSGSAPLDD852 pKa = 3.49VAFDD856 pKa = 4.03ASASYY861 pKa = 11.19DD862 pKa = 3.72PDD864 pKa = 3.96GSIADD869 pKa = 4.05YY870 pKa = 10.9AWDD873 pKa = 3.84YY874 pKa = 11.79GDD876 pKa = 3.93GAVEE880 pKa = 4.27NGASLITPSHH890 pKa = 7.09TYY892 pKa = 8.71TADD895 pKa = 3.14GSYY898 pKa = 10.75SAVLVVTDD906 pKa = 5.23DD907 pKa = 3.94EE908 pKa = 5.1GATATNTTTITVGDD922 pKa = 3.98APEE925 pKa = 4.91GGSFASLTASQTVAGDD941 pKa = 3.63QNVNTATATALDD953 pKa = 4.03WVGSSVDD960 pKa = 3.45ANKK963 pKa = 8.78FTVGANGVITVADD976 pKa = 3.99AGDD979 pKa = 3.75YY980 pKa = 9.82MIAATLPMNGPVYY993 pKa = 9.97RR994 pKa = 11.84SCVRR998 pKa = 11.84MQAYY1002 pKa = 10.07VNGVAVPGLLGEE1014 pKa = 4.19SSYY1017 pKa = 11.11IRR1019 pKa = 11.84DD1020 pKa = 3.36SSGHH1024 pKa = 5.76NEE1026 pKa = 4.21SSSHH1030 pKa = 5.59FAGLLAGLNANDD1042 pKa = 4.09EE1043 pKa = 4.39IQVKK1047 pKa = 10.24VLGTAGSGVITMDD1060 pKa = 3.76GARR1063 pKa = 11.84LYY1065 pKa = 11.16LEE1067 pKa = 5.78AIDD1070 pKa = 4.44ASRR1073 pKa = 11.84AVYY1076 pKa = 10.37SGLSDD1081 pKa = 3.84GPAGGDD1087 pKa = 3.45VNPATAAPMLWTTDD1101 pKa = 3.24QRR1103 pKa = 11.84IDD1105 pKa = 3.57SGFTHH1110 pKa = 6.66SAGSQSITLDD1120 pKa = 3.16ADD1122 pKa = 3.53GLYY1125 pKa = 10.53LVYY1128 pKa = 10.57FNMPIYY1134 pKa = 10.03STVARR1139 pKa = 11.84SAPRR1143 pKa = 11.84IEE1145 pKa = 4.23VKK1147 pKa = 10.73LDD1149 pKa = 3.0GVMVAGGAGKK1159 pKa = 10.17QGDD1162 pKa = 3.75IKK1164 pKa = 11.01SGSGHH1169 pKa = 6.51NDD1171 pKa = 2.82ASVHH1175 pKa = 4.31WSGVIQATAGSVLTVDD1191 pKa = 4.25TMQEE1195 pKa = 3.81ATLGTVTVPAGEE1207 pKa = 4.18KK1208 pKa = 10.88AMLTVEE1214 pKa = 5.19KK1215 pKa = 10.19LDD1217 pKa = 3.86EE1218 pKa = 4.39SQGVYY1223 pKa = 10.3FGLGYY1228 pKa = 8.98TVDD1231 pKa = 5.75GGDD1234 pKa = 3.31SWNVAGNVDD1243 pKa = 4.61FSDD1246 pKa = 5.63LIIDD1250 pKa = 3.56GTKK1253 pKa = 9.3YY1254 pKa = 10.67SKK1256 pKa = 10.12PANHH1260 pKa = 5.64QVQVSEE1266 pKa = 4.33AGDD1269 pKa = 3.58YY1270 pKa = 11.3LLVYY1274 pKa = 10.35NDD1276 pKa = 3.39SFYY1279 pKa = 11.25ATVARR1284 pKa = 11.84ANPKK1288 pKa = 8.81VTVTVNGVPVAGAEE1302 pKa = 4.39VKK1304 pKa = 10.1SHH1306 pKa = 6.55YY1307 pKa = 9.73IRR1309 pKa = 11.84NSGGHH1314 pKa = 5.65NEE1316 pKa = 4.22SSGSLVTMLDD1326 pKa = 3.43GLSAGDD1332 pKa = 3.56VVAIALEE1339 pKa = 4.04QEE1341 pKa = 3.97AAAGIVDD1348 pKa = 4.43DD1349 pKa = 5.35DD1350 pKa = 4.46APAVLALILKK1360 pKa = 7.49TAGPVGPQPPLANAGADD1377 pKa = 3.53QAVEE1381 pKa = 4.08DD1382 pKa = 4.68ADD1384 pKa = 4.89DD1385 pKa = 4.24NGSEE1389 pKa = 4.45SVTLDD1394 pKa = 3.24GTGSTDD1400 pKa = 3.01SDD1402 pKa = 3.9GTIVSYY1408 pKa = 10.93SWTTGTTEE1416 pKa = 3.96IATGAAPVVTLPVGTNVVTLTVEE1439 pKa = 4.84DD1440 pKa = 3.89NDD1442 pKa = 4.04GLQDD1446 pKa = 3.45SDD1448 pKa = 4.17VVVITVNPFVAVNVPPAADD1467 pKa = 3.29AGVDD1471 pKa = 3.66QNVEE1475 pKa = 4.1DD1476 pKa = 4.0TDD1478 pKa = 4.5GNGSEE1483 pKa = 4.64SVSLDD1488 pKa = 3.12GSGSTDD1494 pKa = 2.92SDD1496 pKa = 3.75GTIVSYY1502 pKa = 10.94SWTTGATEE1510 pKa = 3.86IATGVAPVVSLPVGANVVTLTVEE1533 pKa = 4.48DD1534 pKa = 4.61DD1535 pKa = 4.37GGLQDD1540 pKa = 4.15SDD1542 pKa = 4.01TVVITVNPAPANEE1555 pKa = 4.25APVASFTAAPGSGSAPLDD1573 pKa = 3.49VAFDD1577 pKa = 4.03ASASYY1582 pKa = 11.19DD1583 pKa = 3.72PDD1585 pKa = 3.96GSIADD1590 pKa = 4.05YY1591 pKa = 10.9AWDD1594 pKa = 3.84YY1595 pKa = 11.79GDD1597 pKa = 3.93GAVEE1601 pKa = 4.27NGASLITPSHH1611 pKa = 7.09TYY1613 pKa = 8.71TADD1616 pKa = 3.14GSYY1619 pKa = 10.75SAVLVVTDD1627 pKa = 5.23DD1628 pKa = 3.94EE1629 pKa = 5.1GATATNTTTITVGDD1643 pKa = 3.98APEE1646 pKa = 4.91GGSFASLTASQTVAGDD1662 pKa = 3.63QNVNTATATALDD1674 pKa = 4.03WVGSSVDD1681 pKa = 3.45ANKK1684 pKa = 8.78FTVGANGVITVADD1697 pKa = 3.99AGDD1700 pKa = 3.75YY1701 pKa = 9.82MIAATLPMNGPVYY1714 pKa = 9.97RR1715 pKa = 11.84SCVRR1719 pKa = 11.84MQAYY1723 pKa = 10.07VNGVAVPGLLGEE1735 pKa = 4.19SSYY1738 pKa = 11.11IRR1740 pKa = 11.84DD1741 pKa = 3.36SSGHH1745 pKa = 5.76NEE1747 pKa = 4.21SSSHH1751 pKa = 5.59FAGLLAGLNANDD1763 pKa = 4.09EE1764 pKa = 4.39IQVKK1768 pKa = 10.24VLGTAGSGVITMDD1781 pKa = 3.76GARR1784 pKa = 11.84LYY1786 pKa = 11.16LEE1788 pKa = 5.78AIDD1791 pKa = 4.44ASRR1794 pKa = 11.84AVYY1797 pKa = 10.37SGLSDD1802 pKa = 3.84GPAGGDD1808 pKa = 3.45VNPATAAPMLWTTDD1822 pKa = 3.24QRR1824 pKa = 11.84IDD1826 pKa = 3.57SGFTHH1831 pKa = 6.66SAGSQSITLDD1841 pKa = 3.16ADD1843 pKa = 3.53GLYY1846 pKa = 10.53LVYY1849 pKa = 10.57FNMPIYY1855 pKa = 10.03STVARR1860 pKa = 11.84SAPRR1864 pKa = 11.84IEE1866 pKa = 4.23VKK1868 pKa = 10.73LDD1870 pKa = 3.0GVMVAGGAGKK1880 pKa = 9.88QGYY1883 pKa = 8.46IKK1885 pKa = 10.58SGSGHH1890 pKa = 6.47NDD1892 pKa = 2.82ASVHH1896 pKa = 4.31WSGVIQATAGSVLTVDD1912 pKa = 4.25TMQEE1916 pKa = 3.81ATLGTVTVPAGEE1928 pKa = 4.18KK1929 pKa = 10.88AMLTVEE1935 pKa = 5.19KK1936 pKa = 10.19LDD1938 pKa = 3.86EE1939 pKa = 4.39SQGVYY1944 pKa = 10.3FGLGYY1949 pKa = 8.98TVDD1952 pKa = 5.75GGDD1955 pKa = 3.31SWNVAGNVDD1964 pKa = 4.61FSDD1967 pKa = 5.63LIIDD1971 pKa = 3.56GTKK1974 pKa = 9.3YY1975 pKa = 10.67SKK1977 pKa = 10.12PANHH1981 pKa = 5.64QVQVSEE1987 pKa = 4.33AGDD1990 pKa = 3.58YY1991 pKa = 11.3LLVYY1995 pKa = 10.35NDD1997 pKa = 3.39SFYY2000 pKa = 11.25ATVARR2005 pKa = 11.84ANPKK2009 pKa = 8.81VTVTVNGVPVAGAEE2023 pKa = 4.39VKK2025 pKa = 10.1SHH2027 pKa = 6.55YY2028 pKa = 9.73IRR2030 pKa = 11.84NSGGHH2035 pKa = 5.65NEE2037 pKa = 4.22SSGSLVTMLDD2047 pKa = 3.43GLSAGDD2053 pKa = 3.56VVAIALEE2060 pKa = 4.04QEE2062 pKa = 3.97AAAGIVDD2069 pKa = 4.43DD2070 pKa = 5.35DD2071 pKa = 4.46APAVLALISKK2081 pKa = 9.65VSVPTPDD2088 pKa = 4.33PDD2090 pKa = 4.05SDD2092 pKa = 4.11GDD2094 pKa = 4.32GMDD2097 pKa = 4.87DD2098 pKa = 3.62DD2099 pKa = 4.83WEE2101 pKa = 4.14ILHH2104 pKa = 6.76FGNLDD2109 pKa = 3.31QTAEE2113 pKa = 3.95GDD2115 pKa = 3.43YY2116 pKa = 11.34DD2117 pKa = 4.49ADD2119 pKa = 3.54GRR2121 pKa = 11.84TNLQEE2126 pKa = 4.2FQHH2129 pKa = 5.9GTVPTDD2135 pKa = 3.63DD2136 pKa = 3.95QDD2138 pKa = 3.87YY2139 pKa = 10.04QANLTFHH2146 pKa = 6.96VDD2148 pKa = 3.23SLLGNDD2154 pKa = 4.22ANPGTSEE2161 pKa = 5.15LPFATIGRR2169 pKa = 11.84AVGLSIDD2176 pKa = 3.64GDD2178 pKa = 4.34LIEE2181 pKa = 4.89VASGTYY2187 pKa = 9.84PEE2189 pKa = 5.71LPTTYY2194 pKa = 10.94DD2195 pKa = 3.38FTDD2198 pKa = 3.45KK2199 pKa = 10.88NVTMFSTGPYY2209 pKa = 10.01SVTPP2213 pKa = 3.98

Molecular weight:
227.31 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A202DM30|A0A202DM30_9BACT 30S ribosomal protein S17 OS=bacterium E08(2017) OX=1932693 GN=rpsQ PE=3 SV=1
MM1 pKa = 7.57AKK3 pKa = 9.87KK4 pKa = 10.41ALIAKK9 pKa = 8.4SEE11 pKa = 4.11RR12 pKa = 11.84KK13 pKa = 8.92PKK15 pKa = 10.56YY16 pKa = 9.96GVRR19 pKa = 11.84KK20 pKa = 9.13YY21 pKa = 10.54NRR23 pKa = 11.84CKK25 pKa = 9.72RR26 pKa = 11.84CGRR29 pKa = 11.84PRR31 pKa = 11.84AYY33 pKa = 10.13LRR35 pKa = 11.84KK36 pKa = 9.71FEE38 pKa = 4.39LCRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 4.07LASHH50 pKa = 6.33GKK52 pKa = 8.98IPGVIKK58 pKa = 10.76ASWW61 pKa = 2.84

Molecular weight:
7.17 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1267

0

1267

424508

30

3334

335.0

37.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.23 ± 0.071

1.226 ± 0.029

6.373 ± 0.067

6.661 ± 0.076

3.809 ± 0.046

7.626 ± 0.082

1.946 ± 0.034

6.46 ± 0.05

5.815 ± 0.088

9.003 ± 0.083

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.774 ± 0.035

4.011 ± 0.052

4.109 ± 0.044

2.977 ± 0.039

4.973 ± 0.066

6.715 ± 0.067

5.497 ± 0.082

7.181 ± 0.058

1.314 ± 0.029

3.302 ± 0.045

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski