Planctomycetes bacterium HG15A2

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Pirellulales; Lacipirellulaceae; Adhaeretor

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4864 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A517MU95|A0A517MU95_9BACT Phosphorylated carbohydrates phosphatase OS=Planctomycetes bacterium HG15A2 OX=1930276 GN=HG15A2_17280 PE=4 SV=1
MM1 pKa = 6.86NCFYY5 pKa = 10.75IFQTLFNSFKK15 pKa = 9.96GSLRR19 pKa = 11.84FVVAKK24 pKa = 10.25VPLRR28 pKa = 11.84ACVAAAVLADD38 pKa = 3.95MACSASAITIADD50 pKa = 3.43AAGDD54 pKa = 3.83YY55 pKa = 10.43LAAAGSTSTAPLSPPTGWSYY75 pKa = 11.63LGADD79 pKa = 3.93MPNGGTEE86 pKa = 3.75ISLTAGDD93 pKa = 4.36VGNQGASYY101 pKa = 10.02QGFVGGSTAGTAAVYY116 pKa = 7.61GTNTASSLEE125 pKa = 3.91FEE127 pKa = 4.4IFSNGEE133 pKa = 3.7DD134 pKa = 3.16NGAVVGTDD142 pKa = 3.62LLLHH146 pKa = 6.89PGQGGNADD154 pKa = 3.58EE155 pKa = 4.65FVIARR160 pKa = 11.84YY161 pKa = 8.03TISTADD167 pKa = 3.51AANAAAGTGSISGSFRR183 pKa = 11.84EE184 pKa = 4.5LIIGGGAAVDD194 pKa = 4.49SISADD199 pKa = 2.8IYY201 pKa = 11.2HH202 pKa = 5.95NTNNLFSVTGNQSTANALTQAEE224 pKa = 4.87GTFNLTGLTFAEE236 pKa = 4.4NDD238 pKa = 3.79TIDD241 pKa = 3.86FVVGINGHH249 pKa = 6.35FGADD253 pKa = 3.35EE254 pKa = 4.04TALQAIIEE262 pKa = 4.35VEE264 pKa = 4.11QTQPTEE270 pKa = 3.83LLTLQVHH277 pKa = 5.57TLSGDD282 pKa = 3.12VSLINGSQPQDD293 pKa = 2.59IDD295 pKa = 4.11FYY297 pKa = 11.24SIASLGVALNFDD309 pKa = 4.22GWNSLEE315 pKa = 4.34DD316 pKa = 3.54QDD318 pKa = 5.92FEE320 pKa = 5.62GNGAPGTGNGWEE332 pKa = 4.0QSGGSGDD339 pKa = 3.8TVLTEE344 pKa = 4.52AYY346 pKa = 7.99LTGSSVIAADD356 pKa = 3.62SSISIGSAFDD366 pKa = 3.39PTEE369 pKa = 4.3FGAGNDD375 pKa = 3.5GDD377 pKa = 4.52LTFQYY382 pKa = 10.74RR383 pKa = 11.84LADD386 pKa = 3.75GSVVPGLVEE395 pKa = 4.16YY396 pKa = 8.97VTSGDD401 pKa = 3.37FDD403 pKa = 5.89ADD405 pKa = 3.81LDD407 pKa = 3.85IDD409 pKa = 4.42GVDD412 pKa = 4.1FLAWQRR418 pKa = 11.84GFGTMFDD425 pKa = 3.95ASDD428 pKa = 3.79LEE430 pKa = 4.31DD431 pKa = 3.53WQDD434 pKa = 3.02HH435 pKa = 5.77YY436 pKa = 11.84GAGGTSPSLAVAVPEE451 pKa = 4.03PASIVLVIFSLLAGQGFFRR470 pKa = 11.84RR471 pKa = 11.84QSRR474 pKa = 11.84PARR477 pKa = 3.78

Molecular weight: 49.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A517N2B3|A0A517N2B3_9BACT Uncharacterized protein OS=Planctomycetes bacterium HG15A2 OX=1930276 GN=HG15A2_46030 PE=4 SV=1
MM1 pKa = 7.47AAAKK5 pKa = 8.44KK6 pKa = 6.88TAKK9 pKa = 10.27RR10 pKa = 11.84GAAKK14 pKa = 8.91TAKK17 pKa = 10.07KK18 pKa = 10.25SPKK21 pKa = 9.63KK22 pKa = 10.1RR23 pKa = 11.84ATAKK27 pKa = 10.44KK28 pKa = 8.33STAKK32 pKa = 10.42KK33 pKa = 9.78SPKK36 pKa = 9.46KK37 pKa = 10.02RR38 pKa = 11.84AAKK41 pKa = 10.17KK42 pKa = 9.25ATTKK46 pKa = 10.44KK47 pKa = 10.17AAKK50 pKa = 9.79KK51 pKa = 9.97SPKK54 pKa = 10.0KK55 pKa = 10.34KK56 pKa = 8.83ATKK59 pKa = 9.92KK60 pKa = 9.69AAKK63 pKa = 9.85KK64 pKa = 8.79STAKK68 pKa = 9.62RR69 pKa = 11.84TTKK72 pKa = 10.4KK73 pKa = 10.57SPKK76 pKa = 9.38KK77 pKa = 9.53KK78 pKa = 9.86AAAKK82 pKa = 7.94RR83 pKa = 11.84TTKK86 pKa = 10.54KK87 pKa = 9.4STAKK91 pKa = 10.39KK92 pKa = 8.42STAKK96 pKa = 10.36RR97 pKa = 11.84SPKK100 pKa = 9.03KK101 pKa = 7.25TTAKK105 pKa = 10.12RR106 pKa = 11.84GRR108 pKa = 11.84PAKK111 pKa = 10.29KK112 pKa = 9.89KK113 pKa = 10.37

Molecular weight: 12.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4864

0

4864

1702111

29

3026

349.9

38.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.807 ± 0.032

1.106 ± 0.014

5.896 ± 0.035

6.521 ± 0.038

3.681 ± 0.026

8.073 ± 0.041

2.104 ± 0.017

4.818 ± 0.026

3.769 ± 0.032

10.083 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.075 ± 0.017

3.446 ± 0.029

4.949 ± 0.027

4.108 ± 0.026

5.99 ± 0.032

6.68 ± 0.034

5.785 ± 0.031

7.123 ± 0.032

1.477 ± 0.014

2.51 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski