Acyrthosiphon pisum secondary endosymbiont phage 1 (Bacteriophage APSE-1)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Sendosyvirus

Average proteome isoelectric point is 7.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q9T1S2|VP26_BPAPS Putative protein p26 OS=Acyrthosiphon pisum secondary endosymbiont phage 1 OX=67571 GN=26 PE=4 SV=1
MM1 pKa = 7.57TKK3 pKa = 10.19PLTKK7 pKa = 10.75GEE9 pKa = 4.11IVLFALRR16 pKa = 11.84KK17 pKa = 9.96AGIASEE23 pKa = 4.06ATNIDD28 pKa = 3.54VEE30 pKa = 4.33PQSFEE35 pKa = 3.96EE36 pKa = 5.28GINDD40 pKa = 5.03LEE42 pKa = 4.46DD43 pKa = 4.59LMAEE47 pKa = 4.07LQITFGDD54 pKa = 3.66LGYY57 pKa = 10.61QFSAEE62 pKa = 4.23EE63 pKa = 4.09EE64 pKa = 4.38NPTSDD69 pKa = 4.66DD70 pKa = 3.66ASGLPRR76 pKa = 11.84KK77 pKa = 9.08YY78 pKa = 9.79KK79 pKa = 10.43QVMGYY84 pKa = 9.4QLMLRR89 pKa = 11.84MLSDD93 pKa = 3.68YY94 pKa = 10.87GIEE97 pKa = 3.79PTPRR101 pKa = 11.84QEE103 pKa = 3.96ASAAAAYY110 pKa = 8.09DD111 pKa = 3.69ALLTDD116 pKa = 4.15TLSVPSIARR125 pKa = 11.84RR126 pKa = 11.84GDD128 pKa = 3.4MPVGQGNNYY137 pKa = 7.5TALGTASYY145 pKa = 9.24YY146 pKa = 9.37VEE148 pKa = 4.41RR149 pKa = 11.84GFHH152 pKa = 6.76AKK154 pKa = 8.23NTDD157 pKa = 3.4PVSS160 pKa = 3.22

Molecular weight: 17.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q9T1S4|CAPSD_BPAPS Major capsid protein OS=Acyrthosiphon pisum secondary endosymbiont phage 1 OX=67571 GN=24 PE=1 SV=3
MM1 pKa = 7.97RR2 pKa = 11.84IKK4 pKa = 10.92ADD6 pKa = 3.23ISRR9 pKa = 11.84QQVKK13 pKa = 6.79THH15 pKa = 6.34HH16 pKa = 6.94LLVCVTIRR24 pKa = 11.84IFVYY28 pKa = 8.66TNRR31 pKa = 11.84KK32 pKa = 8.28PFIRR36 pKa = 11.84CHH38 pKa = 6.09GGVKK42 pKa = 10.37AMQFWQGDD50 pKa = 4.07GPKK53 pKa = 8.63PTEE56 pKa = 3.7KK57 pKa = 10.77LIVEE61 pKa = 5.07KK62 pKa = 10.66IRR64 pKa = 11.84FHH66 pKa = 7.57LLFSRR71 pKa = 11.84SYY73 pKa = 11.19DD74 pKa = 3.65RR75 pKa = 11.84NCISCLNFCINGIVLRR91 pKa = 11.84IFFIINRR98 pKa = 11.84NTRR101 pKa = 3.02

Molecular weight: 11.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

12525

73

993

231.9

26.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.95 ± 0.402

1.269 ± 0.172

5.453 ± 0.295

5.597 ± 0.357

3.729 ± 0.229

6.116 ± 0.378

2.188 ± 0.191

6.172 ± 0.214

6.587 ± 0.238

9.054 ± 0.274

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.563 ± 0.152

5.054 ± 0.266

4.319 ± 0.256

4.599 ± 0.293

6.044 ± 0.319

6.858 ± 0.346

5.541 ± 0.268

5.365 ± 0.207

1.461 ± 0.206

3.082 ± 0.229
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski