Ralstonia phage RSB1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Okabevirinae; Higashivirus; Ralstonia virus RSB1

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B5BTU2|B5BTU2_9CAUD Uncharacterized protein OS=Ralstonia phage RSB1 OX=551790 PE=4 SV=1
MM1 pKa = 7.48SNDD4 pKa = 3.69LIGFLRR10 pKa = 11.84AWLDD14 pKa = 3.23WAEE17 pKa = 4.71AGAPDD22 pKa = 5.43DD23 pKa = 4.67GRR25 pKa = 11.84HH26 pKa = 4.56TQRR29 pKa = 11.84NPNRR33 pKa = 11.84FDD35 pKa = 4.38RR36 pKa = 11.84DD37 pKa = 3.12VGLCSNTYY45 pKa = 9.75EE46 pKa = 4.15YY47 pKa = 11.27EE48 pKa = 3.96EE49 pKa = 4.57LALDD53 pKa = 5.34RR54 pKa = 11.84LDD56 pKa = 3.94CDD58 pKa = 3.32RR59 pKa = 11.84GEE61 pKa = 5.2LEE63 pKa = 5.9DD64 pKa = 4.24EE65 pKa = 4.09LHH67 pKa = 6.72DD68 pKa = 4.11AFMAEE73 pKa = 4.58GLDD76 pKa = 3.37GTYY79 pKa = 10.71PFGQDD84 pKa = 3.68AYY86 pKa = 11.17DD87 pKa = 4.55DD88 pKa = 4.36GQVMGAQHH96 pKa = 6.22KK97 pKa = 10.77CEE99 pKa = 4.02TRR101 pKa = 11.84LAWVRR106 pKa = 11.84SKK108 pKa = 10.94LAAVNVASTT117 pKa = 3.62

Molecular weight: 13.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B5BTT8|B5BTT8_9CAUD Uncharacterized protein OS=Ralstonia phage RSB1 OX=551790 PE=4 SV=1
MM1 pKa = 7.58ASSHH5 pKa = 5.29TRR7 pKa = 11.84TSARR11 pKa = 11.84HH12 pKa = 4.59GRR14 pKa = 11.84MDD16 pKa = 4.02RR17 pKa = 11.84DD18 pKa = 3.14ATAQCKK24 pKa = 9.66RR25 pKa = 11.84EE26 pKa = 3.68RR27 pKa = 11.84RR28 pKa = 11.84QIGQHH33 pKa = 5.96RR34 pKa = 11.84PSCDD38 pKa = 3.1GANDD42 pKa = 3.86PSTT45 pKa = 3.51

Molecular weight: 5.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

47

0

47

13053

45

1606

277.7

30.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.135 ± 0.663

0.85 ± 0.171

6.389 ± 0.207

5.462 ± 0.363

3.279 ± 0.167

8.312 ± 0.348

1.946 ± 0.233

4.244 ± 0.164

5.133 ± 0.346

7.991 ± 0.29

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.566 ± 0.194

3.93 ± 0.274

4.129 ± 0.225

4.781 ± 0.357

6.037 ± 0.355

5.685 ± 0.331

5.922 ± 0.333

6.604 ± 0.24

1.624 ± 0.151

2.98 ± 0.229
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski