Streptomyces venezuelae (strain ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD 04745)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; Streptomyces venezuelae

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7451 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F2RDY9|F2RDY9_STRVP Uncharacterized protein OS=Streptomyces venezuelae (strain ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD 04745) OX=953739 GN=SVEN_1242 PE=4 SV=1
MM1 pKa = 7.2TVQHH5 pKa = 6.33EE6 pKa = 4.4APSTGAAEE14 pKa = 4.3ALEE17 pKa = 4.09VWIDD21 pKa = 3.41QDD23 pKa = 3.99LCTGDD28 pKa = 5.01GICAQYY34 pKa = 10.9APEE37 pKa = 4.23VFEE40 pKa = 5.9LDD42 pKa = 3.05IDD44 pKa = 3.63GLAYY48 pKa = 10.42VKK50 pKa = 10.8SADD53 pKa = 5.05DD54 pKa = 4.67EE55 pKa = 4.47LLQDD59 pKa = 4.57AGATTPVPLPLLQDD73 pKa = 3.66VVDD76 pKa = 4.35SAKK79 pKa = 10.25EE80 pKa = 3.92CPGDD84 pKa = 4.18CIHH87 pKa = 6.39VRR89 pKa = 11.84RR90 pKa = 11.84VADD93 pKa = 3.43KK94 pKa = 11.16VEE96 pKa = 4.41VYY98 pKa = 10.9GPDD101 pKa = 3.36AEE103 pKa = 4.31

Molecular weight: 10.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F2R917|F2R917_STRVP Uncharacterized protein OS=Streptomyces venezuelae (strain ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD 04745) OX=953739 GN=SVEN_3191 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.72HH17 pKa = 5.74RR18 pKa = 11.84KK19 pKa = 7.77LLKK22 pKa = 8.07RR23 pKa = 11.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.81KK32 pKa = 9.63

Molecular weight: 4.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7451

0

7451

2472419

24

4991

331.8

35.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.953 ± 0.052

0.757 ± 0.007

5.839 ± 0.023

5.749 ± 0.027

2.688 ± 0.015

9.703 ± 0.03

2.238 ± 0.015

2.986 ± 0.02

2.137 ± 0.026

10.336 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.672 ± 0.011

1.697 ± 0.02

6.37 ± 0.029

2.582 ± 0.02

8.083 ± 0.034

4.868 ± 0.02

6.302 ± 0.031

8.435 ± 0.027

1.527 ± 0.013

2.065 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski