Schistosoma mansoni (Blood fluke)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Spiralia; Lophotrochozoa; Platyhelminthes; Trematoda; Digenea; Strigeidida; Schistosomatoidea; Schistosomatidae; Schistosoma

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14182 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q0KNZ1|A0A3Q0KNZ1_SCHMA Amino acid transporter OS=Schistosoma mansoni OX=6183 PE=4 SV=1
MM1 pKa = 7.7LIIVIISSIVYY12 pKa = 9.0INCIVISNNGSYY24 pKa = 10.1QFQSQKK30 pKa = 7.56QTFNANGYY38 pKa = 10.1SMHH41 pKa = 6.57EE42 pKa = 4.2QNQSQSFIHH51 pKa = 6.16NNNDD55 pKa = 3.25GQHH58 pKa = 6.95IIQKK62 pKa = 9.85QSQQQSQHH70 pKa = 6.15SYY72 pKa = 10.5SIQSQIQYY80 pKa = 10.76QSLNYY85 pKa = 9.92TNLQSLVNPQNQNDD99 pKa = 3.94DD100 pKa = 3.5SQIPVDD106 pKa = 3.83TTNQSSGSQISNQAEE121 pKa = 4.33SPSQSGTDD129 pKa = 3.63IDD131 pKa = 4.75PSLPDD136 pKa = 3.14NHH138 pKa = 6.06QQQQQATEE146 pKa = 4.51LPQEE150 pKa = 4.52SQDD153 pKa = 3.74SQQQQQQQQPTQEE166 pKa = 4.32TQNEE170 pKa = 4.15ADD172 pKa = 3.9SPHH175 pKa = 7.2LEE177 pKa = 4.53LSPQFDD183 pKa = 3.3QSHH186 pKa = 6.58SNNSNSILVQPQSSEE201 pKa = 3.91QSQEE205 pKa = 3.88LQDD208 pKa = 4.21NQDD211 pKa = 2.95QSSQQLQSEE220 pKa = 4.54IQQPHH225 pKa = 5.71QLDD228 pKa = 4.46LSPQFDD234 pKa = 3.43QSQSNISDD242 pKa = 5.04LILVQLNSSKK252 pKa = 10.26QSRR255 pKa = 11.84EE256 pKa = 3.59IQDD259 pKa = 3.69NQDD262 pKa = 2.73QSLPQLQPEE271 pKa = 4.5IQQPHH276 pKa = 6.1QIDD279 pKa = 4.06LSSQFDD285 pKa = 3.5QSQSNISDD293 pKa = 5.04LILVQLNSSKK303 pKa = 10.26QSRR306 pKa = 11.84EE307 pKa = 3.72IQDD310 pKa = 3.76SLNQSLPQLQPEE322 pKa = 4.5IQQPHH327 pKa = 6.1QIDD330 pKa = 4.06LSSQFDD336 pKa = 3.5QSQSNISDD344 pKa = 5.1LILVQPQPPGQFQEE358 pKa = 4.24SDD360 pKa = 3.61SQILNVSKK368 pKa = 10.46PVVEE372 pKa = 4.97SYY374 pKa = 11.39SEE376 pKa = 4.13FDD378 pKa = 3.75DD379 pKa = 4.35SQTGEE384 pKa = 4.12PKK386 pKa = 10.26QSLEE390 pKa = 4.08AQLTPDD396 pKa = 4.54PPLSYY401 pKa = 10.9QNSNNNNDD409 pKa = 3.1

Molecular weight: 46.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5K4FAB1|A0A5K4FAB1_SCHMA Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 10.08CQEE5 pKa = 3.82NTKK8 pKa = 9.74PKK10 pKa = 10.27IYY12 pKa = 10.23IFTNATRR19 pKa = 11.84LMSQSSLQQRR29 pKa = 11.84SRR31 pKa = 11.84PQMSQSSLQQRR42 pKa = 11.84SRR44 pKa = 11.84SQMSQSSLQQRR55 pKa = 11.84SRR57 pKa = 11.84PQMSQSSLQQRR68 pKa = 11.84SRR70 pKa = 11.84SQMSQSSLQQRR81 pKa = 11.84SRR83 pKa = 11.84PQMSQSSLQQRR94 pKa = 11.84SRR96 pKa = 11.84SQMSQSSLQQRR107 pKa = 11.84SRR109 pKa = 11.84PQMSQSSLQQRR120 pKa = 11.84SRR122 pKa = 11.84SQMSQSSLQQRR133 pKa = 11.84SRR135 pKa = 11.84PQMSQSS141 pKa = 3.05

Molecular weight: 16.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14095

87

14182

8463562

6

9226

596.8

67.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.468 ± 0.019

2.142 ± 0.013

5.297 ± 0.015

5.581 ± 0.029

3.803 ± 0.014

4.388 ± 0.029

2.896 ± 0.012

6.618 ± 0.024

5.643 ± 0.021

9.697 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.965 ± 0.007

7.228 ± 0.033

4.645 ± 0.024

4.356 ± 0.015

4.84 ± 0.016

10.338 ± 0.037

6.492 ± 0.03

5.326 ± 0.021

0.984 ± 0.005

3.291 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski