Kotonkan virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Ephemerovirus; Kotonkan ephemerovirus

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H8XWF2|H8XWF2_9RHAB Matrix protein OS=Kotonkan virus OX=318836 GN=M PE=4 SV=1
MM1 pKa = 7.59EE2 pKa = 5.86PYY4 pKa = 10.38KK5 pKa = 10.72LSEE8 pKa = 4.17KK9 pKa = 8.22MTGYY13 pKa = 10.99DD14 pKa = 3.44LVKK17 pKa = 10.51LRR19 pKa = 11.84EE20 pKa = 4.09VLNEE24 pKa = 3.87VDD26 pKa = 6.22DD27 pKa = 4.57EE28 pKa = 4.75PEE30 pKa = 3.89DD31 pKa = 3.82TKK33 pKa = 11.32VDD35 pKa = 4.49DD36 pKa = 4.24VTQSPNPLFEE46 pKa = 4.23TSMKK50 pKa = 10.92GNDD53 pKa = 3.53SDD55 pKa = 4.85EE56 pKa = 4.15EE57 pKa = 4.02WGEE60 pKa = 4.11IISNMSNNVCSVNNIINKK78 pKa = 9.84KK79 pKa = 9.55SDD81 pKa = 3.01NDD83 pKa = 3.66APEE86 pKa = 4.07GRR88 pKa = 11.84NDD90 pKa = 3.62VIKK93 pKa = 10.14KK94 pKa = 10.07GRR96 pKa = 11.84MASEE100 pKa = 4.23EE101 pKa = 4.5SNSNLPLMWDD111 pKa = 3.88NNWVMICDD119 pKa = 5.07DD120 pKa = 4.67ISCEE124 pKa = 3.81MSKK127 pKa = 10.36AHH129 pKa = 6.62SLLSLFCLKK138 pKa = 10.8EE139 pKa = 3.8NVDD142 pKa = 3.54YY143 pKa = 11.3KK144 pKa = 10.89FIVNEE149 pKa = 3.88QTLTVQKK156 pKa = 10.91LKK158 pKa = 10.91FNSDD162 pKa = 3.97DD163 pKa = 3.88NPQEE167 pKa = 4.19ASGVTYY173 pKa = 8.58KK174 pKa = 9.76TANYY178 pKa = 10.4SRR180 pKa = 11.84FDD182 pKa = 3.64EE183 pKa = 4.77PEE185 pKa = 4.0EE186 pKa = 4.26SSLKK190 pKa = 10.53YY191 pKa = 10.19KK192 pKa = 10.15IHH194 pKa = 6.04TRR196 pKa = 11.84LDD198 pKa = 3.16EE199 pKa = 4.76GIKK202 pKa = 10.19FKK204 pKa = 10.98KK205 pKa = 10.61LNGKK209 pKa = 10.09GYY211 pKa = 10.18IRR213 pKa = 11.84VSWFTDD219 pKa = 3.56GVYY222 pKa = 10.8QNILDD227 pKa = 5.13DD228 pKa = 4.3IDD230 pKa = 3.67WNNTKK235 pKa = 10.16SEE237 pKa = 4.19EE238 pKa = 4.07EE239 pKa = 3.74AVIRR243 pKa = 11.84VLKK246 pKa = 9.88SAKK249 pKa = 8.83IYY251 pKa = 11.24KK252 pKa = 9.01MIKK255 pKa = 7.81KK256 pKa = 7.74TCEE259 pKa = 3.35II260 pKa = 4.25

Molecular weight: 29.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H8XWF5|H8XWF5_9RHAB Isoform of H8XWF6 Alpha 1 protein OS=Kotonkan virus OX=318836 GN=alpha PE=4 SV=1
MM1 pKa = 7.38NKK3 pKa = 9.47VNWPSLDD10 pKa = 4.13GIKK13 pKa = 9.66LTLKK17 pKa = 9.78EE18 pKa = 4.68FPDD21 pKa = 4.55KK22 pKa = 10.98INTEE26 pKa = 3.61IRR28 pKa = 11.84EE29 pKa = 4.0FSHH32 pKa = 7.14NIVDD36 pKa = 4.52KK37 pKa = 10.88IRR39 pKa = 11.84YY40 pKa = 6.44IWVWLVVIVLLLLIIKK56 pKa = 7.72ITPLLVNLVISCKK69 pKa = 9.83RR70 pKa = 11.84CYY72 pKa = 10.47QNFITGSKK80 pKa = 9.45KK81 pKa = 10.42LKK83 pKa = 10.35EE84 pKa = 4.43EE85 pKa = 3.99EE86 pKa = 4.21TEE88 pKa = 4.06IAA90 pKa = 4.37

Molecular weight: 10.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

1

11

4810

90

2128

437.3

50.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.807 ± 0.349

1.996 ± 0.182

5.904 ± 0.348

7.173 ± 0.362

3.867 ± 0.203

6.071 ± 0.312

1.809 ± 0.188

8.919 ± 0.699

8.898 ± 0.402

9.376 ± 0.746

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.035 ± 0.368

7.006 ± 0.407

3.389 ± 0.226

2.495 ± 0.152

4.262 ± 0.323

6.736 ± 0.499

4.99 ± 0.308

5.301 ± 0.257

1.746 ± 0.17

4.22 ± 0.44
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski