Pseudomonas phage phiNV3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Krylovirinae; Kirikabuvirus; Pseudomonas virus NV3

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P0ZLL8|A0A2P0ZLL8_9CAUD Uncharacterized protein OS=Pseudomonas phage phiNV3 OX=2079544 GN=phiNV3_p47 PE=4 SV=1
MM1 pKa = 7.32KK2 pKa = 10.38QLTQNCNVTKK12 pKa = 10.26EE13 pKa = 4.11DD14 pKa = 3.42WLEE17 pKa = 4.41ADD19 pKa = 4.33VIAQRR24 pKa = 11.84VYY26 pKa = 9.84ITTCCSKK33 pKa = 11.15NEE35 pKa = 4.14GPSVVALTPTQAHH48 pKa = 6.51EE49 pKa = 3.83LAEE52 pKa = 4.13WLEE55 pKa = 4.09QAALEE60 pKa = 4.44AEE62 pKa = 4.74AYY64 pKa = 10.39DD65 pKa = 4.41DD66 pKa = 3.97

Molecular weight: 7.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P0ZLK3|A0A2P0ZLK3_9CAUD Putative internal core protein OS=Pseudomonas phage phiNV3 OX=2079544 GN=phiNV3_p38 PE=4 SV=1
MM1 pKa = 7.06QLHH4 pKa = 6.97DD5 pKa = 3.82VFEE8 pKa = 4.83DD9 pKa = 2.58AGYY12 pKa = 7.69TVRR15 pKa = 11.84RR16 pKa = 11.84KK17 pKa = 9.46VASSKK22 pKa = 9.59TPAGVLVLGTDD33 pKa = 3.25GKK35 pKa = 11.08GYY37 pKa = 10.16YY38 pKa = 8.08RR39 pKa = 11.84TRR41 pKa = 11.84FAGKK45 pKa = 9.43KK46 pKa = 8.58VRR48 pKa = 11.84VHH50 pKa = 6.43NILWEE55 pKa = 4.13LRR57 pKa = 11.84HH58 pKa = 6.08GPVPDD63 pKa = 4.08GMWVDD68 pKa = 4.81HH69 pKa = 6.99INGDD73 pKa = 3.83TLNNHH78 pKa = 7.31DD79 pKa = 4.16YY80 pKa = 10.64NHH82 pKa = 7.03RR83 pKa = 11.84LVSVAQNRR91 pKa = 11.84QNSTKK96 pKa = 10.32QSNNTSGTPGVSWINAKK113 pKa = 8.47QRR115 pKa = 11.84WVVRR119 pKa = 11.84VQANGRR125 pKa = 11.84SHH127 pKa = 7.28SGGQFVNYY135 pKa = 10.43DD136 pKa = 3.83EE137 pKa = 5.22ACAAAAKK144 pKa = 10.12LRR146 pKa = 11.84EE147 pKa = 4.21EE148 pKa = 3.94HH149 pKa = 6.49HH150 pKa = 5.99GAYY153 pKa = 9.93ARR155 pKa = 11.84RR156 pKa = 3.69

Molecular weight: 17.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

12867

45

1310

268.1

29.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.417 ± 0.596

1.142 ± 0.193

6.023 ± 0.203

5.65 ± 0.255

3.225 ± 0.171

8.331 ± 0.384

1.982 ± 0.16

4.173 ± 0.18

4.888 ± 0.265

9.008 ± 0.251

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.891 ± 0.15

3.497 ± 0.24

4.484 ± 0.358

4.554 ± 0.323

6.264 ± 0.26

5.16 ± 0.248

5.93 ± 0.246

6.863 ± 0.325

1.376 ± 0.112

3.14 ± 0.246
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski