Aeromicrobium sp. A1-2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Aeromicrobium; unclassified Aeromicrobium

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3244 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3B7DDU8|A0A3B7DDU8_9ACTN Acetyl-CoA carboxyl transferase OS=Aeromicrobium sp. A1-2 OX=2107713 GN=C6I20_13365 PE=4 SV=1
MM1 pKa = 7.31KK2 pKa = 10.12RR3 pKa = 11.84WEE5 pKa = 4.14CQQCGFIYY13 pKa = 10.57DD14 pKa = 3.86EE15 pKa = 4.62AEE17 pKa = 3.8GWEE20 pKa = 4.39EE21 pKa = 3.67EE22 pKa = 4.94DD23 pKa = 4.39IAPGTKK29 pKa = 9.05WEE31 pKa = 5.71DD32 pKa = 3.45IPDD35 pKa = 3.75DD36 pKa = 4.13WTCPDD41 pKa = 4.16CGAAKK46 pKa = 10.49ADD48 pKa = 3.67FTMLEE53 pKa = 4.35LVV55 pKa = 3.74

Molecular weight: 6.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3B7D0X9|A0A3B7D0X9_9ACTN Phosphoglucosamine mutase OS=Aeromicrobium sp. A1-2 OX=2107713 GN=glmM PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.63KK15 pKa = 9.56KK16 pKa = 9.27HH17 pKa = 5.36RR18 pKa = 11.84KK19 pKa = 5.5MLKK22 pKa = 7.41RR23 pKa = 11.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84LGKK33 pKa = 10.04

Molecular weight: 4.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3244

0

3244

1042278

33

3016

321.3

34.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.499 ± 0.06

0.694 ± 0.011

6.485 ± 0.038

5.537 ± 0.044

3.033 ± 0.025

8.961 ± 0.037

2.197 ± 0.022

4.574 ± 0.033

2.412 ± 0.035

9.984 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.024 ± 0.017

2.004 ± 0.021

5.154 ± 0.033

2.909 ± 0.019

7.101 ± 0.045

5.885 ± 0.031

6.198 ± 0.037

8.945 ± 0.035

1.432 ± 0.019

1.971 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski