Amino acid dipepetide frequency for Methanimicrococcus blatticola

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.304AlaAla: 8.304 ± 0.223
0.867AlaCys: 0.867 ± 0.046
5.514AlaAsp: 5.514 ± 0.113
6.127AlaGlu: 6.127 ± 0.141
3.13AlaPhe: 3.13 ± 0.08
6.494AlaGly: 6.494 ± 0.148
1.175AlaHis: 1.175 ± 0.045
5.157AlaIle: 5.157 ± 0.122
4.78AlaLys: 4.78 ± 0.126
6.599AlaLeu: 6.599 ± 0.137
1.935AlaMet: 1.935 ± 0.075
2.636AlaAsn: 2.636 ± 0.077
2.576AlaPro: 2.576 ± 0.086
1.91AlaGln: 1.91 ± 0.071
2.542AlaArg: 2.542 ± 0.081
4.137AlaSer: 4.137 ± 0.112
3.665AlaThr: 3.665 ± 0.106
7.145AlaVal: 7.145 ± 0.146
0.5AlaTrp: 0.5 ± 0.032
2.292AlaTyr: 2.292 ± 0.072
0.0AlaXaa: 0.0 ± 0.0
Cys
0.886CysAla: 0.886 ± 0.054
0.338CysCys: 0.338 ± 0.047
0.747CysAsp: 0.747 ± 0.046
0.611CysGlu: 0.611 ± 0.058
0.489CysPhe: 0.489 ± 0.033
1.295CysGly: 1.295 ± 0.068
0.239CysHis: 0.239 ± 0.021
0.714CysIle: 0.714 ± 0.039
0.626CysLys: 0.626 ± 0.04
0.955CysLeu: 0.955 ± 0.047
0.296CysMet: 0.296 ± 0.025
0.439CysAsn: 0.439 ± 0.042
0.728CysPro: 0.728 ± 0.044
0.298CysGln: 0.298 ± 0.028
0.571CysArg: 0.571 ± 0.045
0.718CysSer: 0.718 ± 0.044
0.527CysThr: 0.527 ± 0.039
0.817CysVal: 0.817 ± 0.051
0.065CysTrp: 0.065 ± 0.01
0.497CysTyr: 0.497 ± 0.038
0.0CysXaa: 0.0 ± 0.0
Asp
4.568AspAla: 4.568 ± 0.103
0.684AspCys: 0.684 ± 0.053
3.55AspAsp: 3.55 ± 0.105
5.082AspGlu: 5.082 ± 0.119
3.031AspPhe: 3.031 ± 0.083
4.656AspGly: 4.656 ± 0.135
0.827AspHis: 0.827 ± 0.046
4.882AspIle: 4.882 ± 0.122
3.667AspLys: 3.667 ± 0.096
5.462AspLeu: 5.462 ± 0.108
1.728AspMet: 1.728 ± 0.067
2.319AspAsn: 2.319 ± 0.093
2.412AspPro: 2.412 ± 0.076
1.345AspGln: 1.345 ± 0.057
2.177AspArg: 2.177 ± 0.073
3.797AspSer: 3.797 ± 0.108
2.699AspThr: 2.699 ± 0.079
4.79AspVal: 4.79 ± 0.105
0.594AspTrp: 0.594 ± 0.041
2.403AspTyr: 2.403 ± 0.079
0.002AspXaa: 0.002 ± 0.002
Glu
5.149GluAla: 5.149 ± 0.118
0.722GluCys: 0.722 ± 0.045
4.013GluAsp: 4.013 ± 0.107
6.285GluGlu: 6.285 ± 0.175
2.932GluPhe: 2.932 ± 0.084
3.749GluGly: 3.749 ± 0.098
0.993GluHis: 0.993 ± 0.047
7.17GluIle: 7.17 ± 0.157
7.068GluLys: 7.068 ± 0.154
5.995GluLeu: 5.995 ± 0.124
2.748GluMet: 2.748 ± 0.077
4.813GluAsn: 4.813 ± 0.113
2.114GluPro: 2.114 ± 0.076
1.95GluGln: 1.95 ± 0.08
2.779GluArg: 2.779 ± 0.099
4.33GluSer: 4.33 ± 0.095
4.756GluThr: 4.756 ± 0.099
4.032GluVal: 4.032 ± 0.1
0.529GluTrp: 0.529 ± 0.034
2.676GluTyr: 2.676 ± 0.084
0.0GluXaa: 0.0 ± 0.0
Phe
3.035PheAla: 3.035 ± 0.092
0.525PheCys: 0.525 ± 0.041
3.058PheAsp: 3.058 ± 0.081
3.329PheGlu: 3.329 ± 0.091
1.944PhePhe: 1.944 ± 0.078
3.258PheGly: 3.258 ± 0.095
0.586PheHis: 0.586 ± 0.037
3.258PheIle: 3.258 ± 0.111
2.785PheLys: 2.785 ± 0.084
3.764PheLeu: 3.764 ± 0.1
1.14PheMet: 1.14 ± 0.047
1.776PheAsn: 1.776 ± 0.069
1.446PhePro: 1.446 ± 0.065
0.961PheGln: 0.961 ± 0.044
1.329PheArg: 1.329 ± 0.052
3.262PheSer: 3.262 ± 0.089
2.328PheThr: 2.328 ± 0.082
3.14PheVal: 3.14 ± 0.099
0.365PheTrp: 0.365 ± 0.029
1.57PheTyr: 1.57 ± 0.068
0.0PheXaa: 0.0 ± 0.0
Gly
5.38GlyAla: 5.38 ± 0.141
1.083GlyCys: 1.083 ± 0.062
4.162GlyAsp: 4.162 ± 0.117
4.288GlyGlu: 4.288 ± 0.106
3.371GlyPhe: 3.371 ± 0.077
5.835GlyGly: 5.835 ± 0.23
1.157GlyHis: 1.157 ± 0.048
6.173GlyIle: 6.173 ± 0.127
5.468GlyLys: 5.468 ± 0.11
5.724GlyLeu: 5.724 ± 0.13
1.963GlyMet: 1.963 ± 0.08
3.451GlyAsn: 3.451 ± 0.136
1.95GlyPro: 1.95 ± 0.059
1.564GlyGln: 1.564 ± 0.068
2.664GlyArg: 2.664 ± 0.072
4.74GlySer: 4.74 ± 0.149
4.796GlyThr: 4.796 ± 0.141
5.073GlyVal: 5.073 ± 0.128
0.743GlyTrp: 0.743 ± 0.053
2.582GlyTyr: 2.582 ± 0.084
0.0GlyXaa: 0.0 ± 0.0
His
1.083HisAla: 1.083 ± 0.05
0.254HisCys: 0.254 ± 0.026
0.877HisAsp: 0.877 ± 0.046
1.016HisGlu: 1.016 ± 0.051
0.688HisPhe: 0.688 ± 0.039
1.033HisGly: 1.033 ± 0.053
0.334HisHis: 0.334 ± 0.028
1.222HisIle: 1.222 ± 0.053
0.875HisLys: 0.875 ± 0.041
1.373HisLeu: 1.373 ± 0.064
0.464HisMet: 0.464 ± 0.029
0.563HisAsn: 0.563 ± 0.037
0.936HisPro: 0.936 ± 0.041
0.414HisGln: 0.414 ± 0.028
0.596HisArg: 0.596 ± 0.038
0.947HisSer: 0.947 ± 0.05
0.856HisThr: 0.856 ± 0.043
1.14HisVal: 1.14 ± 0.049
0.132HisTrp: 0.132 ± 0.018
0.594HisTyr: 0.594 ± 0.035
0.0HisXaa: 0.0 ± 0.0
Ile
6.119IleAla: 6.119 ± 0.126
1.012IleCys: 1.012 ± 0.048
4.763IleAsp: 4.763 ± 0.106
5.905IleGlu: 5.905 ± 0.128
3.302IlePhe: 3.302 ± 0.099
6.104IleGly: 6.104 ± 0.142
1.312IleHis: 1.312 ± 0.048
5.804IleIle: 5.804 ± 0.141
4.318IleLys: 4.318 ± 0.09
7.303IleLeu: 7.303 ± 0.157
1.906IleMet: 1.906 ± 0.065
3.063IleAsn: 3.063 ± 0.097
3.552IlePro: 3.552 ± 0.078
2.468IleGln: 2.468 ± 0.079
3.298IleArg: 3.298 ± 0.101
5.615IleSer: 5.615 ± 0.116
4.33IleThr: 4.33 ± 0.102
5.443IleVal: 5.443 ± 0.098
0.596IleTrp: 0.596 ± 0.037
2.746IleTyr: 2.746 ± 0.076
0.0IleXaa: 0.0 ± 0.0
Lys
5.115LysAla: 5.115 ± 0.12
0.749LysCys: 0.749 ± 0.047
4.089LysAsp: 4.089 ± 0.092
6.354LysGlu: 6.354 ± 0.148
2.095LysPhe: 2.095 ± 0.068
3.864LysGly: 3.864 ± 0.091
1.012LysHis: 1.012 ± 0.047
5.846LysIle: 5.846 ± 0.112
6.22LysLys: 6.22 ± 0.151
4.801LysLeu: 4.801 ± 0.101
2.464LysMet: 2.464 ± 0.074
4.028LysAsn: 4.028 ± 0.1
2.479LysPro: 2.479 ± 0.081
1.818LysGln: 1.818 ± 0.064
2.869LysArg: 2.869 ± 0.084
4.236LysSer: 4.236 ± 0.089
5.13LysThr: 5.13 ± 0.115
3.661LysVal: 3.661 ± 0.099
0.504LysTrp: 0.504 ± 0.036
2.609LysTyr: 2.609 ± 0.079
0.0LysXaa: 0.0 ± 0.0
Leu
6.295LeuAla: 6.295 ± 0.13
0.877LeuCys: 0.877 ± 0.043
4.738LeuAsp: 4.738 ± 0.097
5.607LeuGlu: 5.607 ± 0.136
3.946LeuPhe: 3.946 ± 0.126
5.38LeuGly: 5.38 ± 0.13
1.251LeuHis: 1.251 ± 0.056
6.96LeuIle: 6.96 ± 0.142
6.406LeuLys: 6.406 ± 0.133
7.796LeuLeu: 7.796 ± 0.198
2.441LeuMet: 2.441 ± 0.079
3.923LeuAsn: 3.923 ± 0.093
3.699LeuPro: 3.699 ± 0.094
1.971LeuGln: 1.971 ± 0.061
3.275LeuArg: 3.275 ± 0.117
6.261LeuSer: 6.261 ± 0.138
4.966LeuThr: 4.966 ± 0.111
5.029LeuVal: 5.029 ± 0.113
0.56LeuTrp: 0.56 ± 0.037
2.739LeuTyr: 2.739 ± 0.084
0.0LeuXaa: 0.0 ± 0.0
Met
2.154MetAla: 2.154 ± 0.075
0.283MetCys: 0.283 ± 0.023
1.61MetAsp: 1.61 ± 0.064
1.914MetGlu: 1.914 ± 0.062
0.959MetPhe: 0.959 ± 0.048
1.633MetGly: 1.633 ± 0.072
0.411MetHis: 0.411 ± 0.028
2.332MetIle: 2.332 ± 0.082
2.322MetLys: 2.322 ± 0.075
1.99MetLeu: 1.99 ± 0.07
0.779MetMet: 0.779 ± 0.041
1.446MetAsn: 1.446 ± 0.055
1.159MetPro: 1.159 ± 0.043
0.682MetGln: 0.682 ± 0.035
1.077MetArg: 1.077 ± 0.044
1.799MetSer: 1.799 ± 0.06
2.259MetThr: 2.259 ± 0.064
1.753MetVal: 1.753 ± 0.07
0.162MetTrp: 0.162 ± 0.015
0.701MetTyr: 0.701 ± 0.037
0.0MetXaa: 0.0 ± 0.0
Asn
3.696AsnAla: 3.696 ± 0.096
0.588AsnCys: 0.588 ± 0.042
2.525AsnAsp: 2.525 ± 0.076
3.293AsnGlu: 3.293 ± 0.096
1.688AsnPhe: 1.688 ± 0.059
3.612AsnGly: 3.612 ± 0.115
0.651AsnHis: 0.651 ± 0.037
3.627AsnIle: 3.627 ± 0.111
2.756AsnLys: 2.756 ± 0.086
3.724AsnLeu: 3.724 ± 0.096
1.28AsnMet: 1.28 ± 0.045
2.277AsnAsn: 2.277 ± 0.114
2.366AsnPro: 2.366 ± 0.079
1.369AsnGln: 1.369 ± 0.059
1.736AsnArg: 1.736 ± 0.059
3.044AsnSer: 3.044 ± 0.105
2.378AsnThr: 2.378 ± 0.094
3.302AsnVal: 3.302 ± 0.105
0.397AsnTrp: 0.397 ± 0.033
1.566AsnTyr: 1.566 ± 0.07
0.0AsnXaa: 0.0 ± 0.0
Pro
3.272ProAla: 3.272 ± 0.095
0.405ProCys: 0.405 ± 0.029
2.882ProAsp: 2.882 ± 0.073
3.94ProGlu: 3.94 ± 0.096
1.719ProPhe: 1.719 ± 0.067
2.67ProGly: 2.67 ± 0.083
0.674ProHis: 0.674 ± 0.04
2.697ProIle: 2.697 ± 0.085
2.21ProLys: 2.21 ± 0.074
3.157ProLeu: 3.157 ± 0.089
0.884ProMet: 0.884 ± 0.044
1.45ProAsn: 1.45 ± 0.067
1.455ProPro: 1.455 ± 0.063
0.928ProGln: 0.928 ± 0.045
1.165ProArg: 1.165 ± 0.056
2.17ProSer: 2.17 ± 0.074
2.143ProThr: 2.143 ± 0.075
3.344ProVal: 3.344 ± 0.087
0.25ProTrp: 0.25 ± 0.022
1.379ProTyr: 1.379 ± 0.061
0.0ProXaa: 0.0 ± 0.0
Gln
1.65GlnAla: 1.65 ± 0.062
0.239GlnCys: 0.239 ± 0.023
1.033GlnAsp: 1.033 ± 0.041
1.688GlnGlu: 1.688 ± 0.06
0.978GlnPhe: 0.978 ± 0.047
1.178GlnGly: 1.178 ± 0.053
0.321GlnHis: 0.321 ± 0.027
2.557GlnIle: 2.557 ± 0.078
2.869GlnLys: 2.869 ± 0.069
2.028GlnLeu: 2.028 ± 0.071
0.825GlnMet: 0.825 ± 0.039
1.654GlnAsn: 1.654 ± 0.062
0.882GlnPro: 0.882 ± 0.046
0.653GlnGln: 0.653 ± 0.04
1.092GlnArg: 1.092 ± 0.046
1.589GlnSer: 1.589 ± 0.06
1.837GlnThr: 1.837 ± 0.059
1.312GlnVal: 1.312 ± 0.053
0.229GlnTrp: 0.229 ± 0.033
0.859GlnTyr: 0.859 ± 0.043
0.0GlnXaa: 0.0 ± 0.0
Arg
2.68ArgAla: 2.68 ± 0.084
0.508ArgCys: 0.508 ± 0.042
2.183ArgAsp: 2.183 ± 0.077
2.995ArgGlu: 2.995 ± 0.091
1.704ArgPhe: 1.704 ± 0.066
2.334ArgGly: 2.334 ± 0.082
0.611ArgHis: 0.611 ± 0.037
3.117ArgIle: 3.117 ± 0.08
2.874ArgLys: 2.874 ± 0.084
3.392ArgLeu: 3.392 ± 0.088
1.003ArgMet: 1.003 ± 0.04
1.793ArgAsn: 1.793 ± 0.064
1.45ArgPro: 1.45 ± 0.059
1.073ArgGln: 1.073 ± 0.05
1.814ArgArg: 1.814 ± 0.064
2.137ArgSer: 2.137 ± 0.073
1.807ArgThr: 1.807 ± 0.06
2.489ArgVal: 2.489 ± 0.078
0.3ArgTrp: 0.3 ± 0.03
1.406ArgTyr: 1.406 ± 0.056
0.0ArgXaa: 0.0 ± 0.0
Ser
4.958SerAla: 4.958 ± 0.111
0.686SerCys: 0.686 ± 0.04
4.309SerAsp: 4.309 ± 0.108
4.687SerGlu: 4.687 ± 0.117
3.039SerPhe: 3.039 ± 0.082
5.976SerGly: 5.976 ± 0.151
1.06SerHis: 1.06 ± 0.047
4.412SerIle: 4.412 ± 0.116
4.446SerLys: 4.446 ± 0.103
4.989SerLeu: 4.989 ± 0.103
1.539SerMet: 1.539 ± 0.064
2.84SerAsn: 2.84 ± 0.105
2.256SerPro: 2.256 ± 0.073
1.602SerGln: 1.602 ± 0.062
2.309SerArg: 2.309 ± 0.067
4.217SerSer: 4.217 ± 0.143
3.266SerThr: 3.266 ± 0.099
5.103SerVal: 5.103 ± 0.096
0.502SerTrp: 0.502 ± 0.033
2.12SerTyr: 2.12 ± 0.071
0.0SerXaa: 0.0 ± 0.0
Thr
5.002ThrAla: 5.002 ± 0.103
0.497ThrCys: 0.497 ± 0.037
3.69ThrAsp: 3.69 ± 0.084
4.053ThrGlu: 4.053 ± 0.091
2.527ThrPhe: 2.527 ± 0.092
4.939ThrGly: 4.939 ± 0.135
0.959ThrHis: 0.959 ± 0.041
4.486ThrIle: 4.486 ± 0.104
3.48ThrLys: 3.48 ± 0.09
4.605ThrLeu: 4.605 ± 0.107
1.19ThrMet: 1.19 ± 0.054
2.389ThrAsn: 2.389 ± 0.1
2.859ThrPro: 2.859 ± 0.091
1.438ThrGln: 1.438 ± 0.059
1.935ThrArg: 1.935 ± 0.067
3.17ThrSer: 3.17 ± 0.093
3.34ThrThr: 3.34 ± 0.118
5.256ThrVal: 5.256 ± 0.121
0.405ThrTrp: 0.405 ± 0.034
2.08ThrTyr: 2.08 ± 0.116
0.002ThrXaa: 0.002 ± 0.002
Val
4.95ValAla: 4.95 ± 0.103
1.043ValCys: 1.043 ± 0.057
3.988ValAsp: 3.988 ± 0.101
4.586ValGlu: 4.586 ± 0.112
3.249ValPhe: 3.249 ± 0.084
5.082ValGly: 5.082 ± 0.127
1.108ValHis: 1.108 ± 0.045
5.527ValIle: 5.527 ± 0.118
4.276ValLys: 4.276 ± 0.099
6.717ValLeu: 6.717 ± 0.134
1.776ValMet: 1.776 ± 0.064
2.876ValAsn: 2.876 ± 0.089
2.853ValPro: 2.853 ± 0.082
1.816ValGln: 1.816 ± 0.064
2.802ValArg: 2.802 ± 0.09
5.271ValSer: 5.271 ± 0.107
4.547ValThr: 4.547 ± 0.134
5.097ValVal: 5.097 ± 0.115
0.644ValTrp: 0.644 ± 0.045
2.534ValTyr: 2.534 ± 0.079
0.0ValXaa: 0.0 ± 0.0
Trp
0.502TrpAla: 0.502 ± 0.036
0.09TrpCys: 0.09 ± 0.013
0.554TrpAsp: 0.554 ± 0.041
0.558TrpGlu: 0.558 ± 0.034
0.365TrpPhe: 0.365 ± 0.025
0.493TrpGly: 0.493 ± 0.034
0.094TrpHis: 0.094 ± 0.015
0.628TrpIle: 0.628 ± 0.041
0.6TrpLys: 0.6 ± 0.041
0.678TrpLeu: 0.678 ± 0.043
0.227TrpMet: 0.227 ± 0.02
0.474TrpAsn: 0.474 ± 0.036
0.193TrpPro: 0.193 ± 0.022
0.264TrpGln: 0.264 ± 0.023
0.277TrpArg: 0.277 ± 0.026
0.483TrpSer: 0.483 ± 0.043
0.516TrpThr: 0.516 ± 0.038
0.449TrpVal: 0.449 ± 0.034
0.086TrpTrp: 0.086 ± 0.013
0.357TrpTyr: 0.357 ± 0.031
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.691TyrAla: 2.691 ± 0.083
0.403TyrCys: 0.403 ± 0.028
2.387TyrAsp: 2.387 ± 0.075
2.487TyrGlu: 2.487 ± 0.088
1.7TyrPhe: 1.7 ± 0.06
2.58TyrGly: 2.58 ± 0.084
0.621TyrHis: 0.621 ± 0.034
2.389TyrIle: 2.389 ± 0.076
1.898TyrLys: 1.898 ± 0.067
3.069TyrLeu: 3.069 ± 0.092
0.835TyrMet: 0.835 ± 0.049
1.665TyrAsn: 1.665 ± 0.064
1.432TyrPro: 1.432 ± 0.063
0.947TyrGln: 0.947 ± 0.04
1.366TyrArg: 1.366 ± 0.055
2.393TyrSer: 2.393 ± 0.092
2.154TyrThr: 2.154 ± 0.076
2.368TyrVal: 2.368 ± 0.08
0.327TyrTrp: 0.327 ± 0.029
1.511TyrTyr: 1.511 ± 0.064
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.002XaaAsp: 0.002 ± 0.002
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.002XaaSer: 0.002 ± 0.002
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.004XaaXaa: 0.004 ± 0.003
Statistics based on 1537 proteins (476405 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski