Babesia sp. Xinjiang

Taxonomy: cellular organisms; Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Piroplasmida; Babesiidae; Babesia; unclassified Babesia

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2856 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X1BLA4|A0A1X1BLA4_9APIC Calcium-dependent protein kinase 2 OS=Babesia sp. Xinjiang OX=462227 GN=BXIN_1623 PE=4 SV=1
MM1 pKa = 8.02DD2 pKa = 4.62NLEE5 pKa = 4.37SEE7 pKa = 4.84HH8 pKa = 6.6VAEE11 pKa = 4.63IDD13 pKa = 3.31SRR15 pKa = 11.84YY16 pKa = 10.69APIVSNNEE24 pKa = 3.4TADD27 pKa = 3.49YY28 pKa = 10.78HH29 pKa = 5.59EE30 pKa = 4.45QLMAGANTPRR40 pKa = 11.84GEE42 pKa = 3.97MDD44 pKa = 4.4DD45 pKa = 6.33DD46 pKa = 3.7EE47 pKa = 4.82FQYY50 pKa = 11.41GEE52 pKa = 4.3PPRR55 pKa = 11.84VFYY58 pKa = 11.23DD59 pKa = 3.74NMDD62 pKa = 3.72SLDD65 pKa = 4.19AVPEE69 pKa = 4.08LLGLPDD75 pKa = 5.84DD76 pKa = 5.52IISQFPVTDD85 pKa = 4.25FDD87 pKa = 3.97AAAAEE92 pKa = 4.43TWNEE96 pKa = 3.86DD97 pKa = 3.02AKK99 pKa = 11.05QCSICLDD106 pKa = 3.75AYY108 pKa = 10.32EE109 pKa = 3.97QDD111 pKa = 3.28QLIRR115 pKa = 11.84RR116 pKa = 11.84LACTHH121 pKa = 7.18GYY123 pKa = 10.25HH124 pKa = 6.79KK125 pKa = 11.07GCIDD129 pKa = 3.26EE130 pKa = 4.21WLSRR134 pKa = 11.84STVCPICKK142 pKa = 9.01FDD144 pKa = 3.57YY145 pKa = 10.59RR146 pKa = 11.84IMMM149 pKa = 5.08

Molecular weight: 17.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X1BJR0|A0A1X1BJR0_9APIC 60S ribosomal protein L3 OS=Babesia sp. Xinjiang OX=462227 GN=BXIN_2195 PE=3 SV=1
MM1 pKa = 7.46IPRR4 pKa = 11.84YY5 pKa = 9.43KK6 pKa = 10.25PKK8 pKa = 10.33NRR10 pKa = 11.84RR11 pKa = 11.84SCWLEE16 pKa = 3.45GCPQKK21 pKa = 10.9KK22 pKa = 9.49GVCVTIRR29 pKa = 11.84VVTPRR34 pKa = 11.84KK35 pKa = 9.24PNSGLRR41 pKa = 11.84KK42 pKa = 7.64VARR45 pKa = 11.84VRR47 pKa = 11.84LSTGRR52 pKa = 11.84TVTCHH57 pKa = 5.8VPGEE61 pKa = 4.19GHH63 pKa = 6.41NLHH66 pKa = 5.8THH68 pKa = 4.77SVVLVRR74 pKa = 11.84GGRR77 pKa = 11.84CQDD80 pKa = 3.11VSGCHH85 pKa = 4.99YY86 pKa = 10.49KK87 pKa = 10.63VIRR90 pKa = 11.84GKK92 pKa = 10.98YY93 pKa = 9.74DD94 pKa = 3.79LLPVKK99 pKa = 10.41NRR101 pKa = 11.84ASSRR105 pKa = 11.84SKK107 pKa = 11.18YY108 pKa = 9.64GVKK111 pKa = 10.62LSDD114 pKa = 3.06AVVNKK119 pKa = 10.03RR120 pKa = 11.84RR121 pKa = 11.84LRR123 pKa = 11.84MNQLHH128 pKa = 5.75VTTEE132 pKa = 3.88LDD134 pKa = 3.01RR135 pKa = 11.84DD136 pKa = 4.01VFNTLMLQDD145 pKa = 3.79WVNVDD150 pKa = 3.06GSRR153 pKa = 11.84RR154 pKa = 11.84TEE156 pKa = 4.4PIAPDD161 pKa = 3.34EE162 pKa = 4.33PVPVDD167 pKa = 3.1IFSFNSRR174 pKa = 11.84LRR176 pKa = 11.84NIVHH180 pKa = 6.53ANKK183 pKa = 10.56GG184 pKa = 3.45

Molecular weight: 20.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2856

0

2856

1634980

59

6701

572.5

64.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.685 ± 0.031

2.148 ± 0.022

6.05 ± 0.027

6.311 ± 0.039

3.868 ± 0.026

5.583 ± 0.027

2.604 ± 0.014

5.586 ± 0.028

5.673 ± 0.036

9.66 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.545 ± 0.02

4.493 ± 0.023

4.318 ± 0.027

3.523 ± 0.025

6.005 ± 0.034

7.927 ± 0.05

5.748 ± 0.023

6.899 ± 0.03

0.984 ± 0.011

3.389 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski