Enterobacter phage Ec_L1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Eclunavirus; Enterobacter virus EcL1

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 85 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P0W9Z4|A0A2P0W9Z4_9CAUD Tail assembly protein OS=Enterobacter phage Ec_L1 OX=2070180 GN=Ec77 PE=4 SV=1
MM1 pKa = 7.43SKK3 pKa = 9.88IKK5 pKa = 10.57KK6 pKa = 10.06GDD8 pKa = 3.33IIKK11 pKa = 10.6CVTSLMDD18 pKa = 3.42GCFMAGCEE26 pKa = 4.12YY27 pKa = 10.23PVEE30 pKa = 4.47KK31 pKa = 10.62VDD33 pKa = 3.51SDD35 pKa = 3.72GFAFVYY41 pKa = 10.56NDD43 pKa = 3.72NGEE46 pKa = 4.02QCAIDD51 pKa = 4.26YY52 pKa = 9.46PHH54 pKa = 7.43DD55 pKa = 4.37PDD57 pKa = 3.44YY58 pKa = 11.87GKK60 pKa = 10.73FEE62 pKa = 4.33KK63 pKa = 10.78VDD65 pKa = 3.24

Molecular weight: 7.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P0W9W0|A0A2P0W9W0_9CAUD Uncharacterized protein OS=Enterobacter phage Ec_L1 OX=2070180 GN=Ec23 PE=4 SV=1
MM1 pKa = 7.52ISAEE5 pKa = 3.82AWVFRR10 pKa = 11.84AGFYY14 pKa = 11.04AKK16 pKa = 9.92IGFDD20 pKa = 3.71FYY22 pKa = 10.68IWHH25 pKa = 7.57PIKK28 pKa = 10.53KK29 pKa = 8.69IWIRR33 pKa = 11.84IYY35 pKa = 8.9WLCGNTYY42 pKa = 10.04EE43 pKa = 5.45RR44 pKa = 11.84ARR46 pKa = 11.84CNRR49 pKa = 11.84KK50 pKa = 9.26LMKK53 pKa = 10.55VKK55 pKa = 10.31ILLEE59 pKa = 4.33KK60 pKa = 10.4IRR62 pKa = 11.84LL63 pKa = 3.68

Molecular weight: 7.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

85

0

85

15645

51

1300

184.1

20.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.661 ± 0.474

1.4 ± 0.198

6.168 ± 0.265

6.718 ± 0.235

3.976 ± 0.171

7.53 ± 0.29

1.918 ± 0.207

6.226 ± 0.178

7.242 ± 0.266

6.59 ± 0.298

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.017 ± 0.143

4.743 ± 0.258

3.221 ± 0.197

3.535 ± 0.274

4.915 ± 0.21

6.379 ± 0.278

5.471 ± 0.247

7.357 ± 0.343

1.477 ± 0.111

3.458 ± 0.14
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski