Streptomyces phage Yosif

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Arquatrovirinae; Camvirus; unclassified Camvirus

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z4QD12|A0A2Z4QD12_9CAUD Uncharacterized protein OS=Streptomyces phage Yosif OX=2201421 GN=6 PE=4 SV=1
MM1 pKa = 7.41SLQVTTWDD9 pKa = 2.93GHH11 pKa = 4.62TFIITKK17 pKa = 10.26RR18 pKa = 11.84EE19 pKa = 3.9DD20 pKa = 3.28TTTVSNLIEE29 pKa = 4.01TAFNDD34 pKa = 3.03IAKK37 pKa = 10.21LVEE40 pKa = 3.82PHH42 pKa = 6.78LNEE45 pKa = 4.07LPEE48 pKa = 4.15GVRR51 pKa = 11.84AILEE55 pKa = 4.3GLVEE59 pKa = 4.17TASDD63 pKa = 3.45DD64 pKa = 3.93AYY66 pKa = 11.44QDD68 pKa = 3.58GHH70 pKa = 7.48SDD72 pKa = 3.83GYY74 pKa = 11.21SEE76 pKa = 4.36GEE78 pKa = 3.59EE79 pKa = 3.82WGFQDD84 pKa = 4.16GQEE87 pKa = 4.01SGYY90 pKa = 10.47QDD92 pKa = 4.7GYY94 pKa = 10.22DD95 pKa = 3.1TGYY98 pKa = 11.22DD99 pKa = 3.2EE100 pKa = 5.94GFEE103 pKa = 4.24AGQEE107 pKa = 3.95EE108 pKa = 4.79AAA110 pKa = 4.47

Molecular weight: 12.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z4QCJ1|A0A2Z4QCJ1_9CAUD Uncharacterized protein OS=Streptomyces phage Yosif OX=2201421 GN=72 PE=4 SV=1
MM1 pKa = 6.87KK2 pKa = 9.69TVRR5 pKa = 11.84HH6 pKa = 5.32TSCRR10 pKa = 11.84CGVKK14 pKa = 10.03RR15 pKa = 11.84GFMSKK20 pKa = 9.35RR21 pKa = 11.84DD22 pKa = 3.39ADD24 pKa = 3.85KK25 pKa = 11.44ALGRR29 pKa = 11.84AQAKK33 pKa = 9.55RR34 pKa = 11.84NRR36 pKa = 11.84AAEE39 pKa = 4.15STGTRR44 pKa = 11.84RR45 pKa = 11.84GLRR48 pKa = 11.84VEE50 pKa = 3.78QRR52 pKa = 11.84YY53 pKa = 8.74YY54 pKa = 10.98EE55 pKa = 4.41CDD57 pKa = 3.08FGMWHH62 pKa = 6.4LTSEE66 pKa = 4.08NRR68 pKa = 11.84KK69 pKa = 8.85SYY71 pKa = 9.49EE72 pKa = 3.77SRR74 pKa = 11.84MVAAA78 pKa = 5.77

Molecular weight: 9.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

78

0

78

15248

39

1337

195.5

21.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.46 ± 0.332

0.675 ± 0.102

6.421 ± 0.32

7.26 ± 0.448

2.991 ± 0.154

8.493 ± 0.385

2.007 ± 0.223

4.427 ± 0.317

4.722 ± 0.337

8.362 ± 0.398

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.19 ± 0.128

2.945 ± 0.158

4.853 ± 0.246

3.351 ± 0.134

6.558 ± 0.439

6.047 ± 0.255

6.171 ± 0.318

7.312 ± 0.23

1.869 ± 0.124

2.886 ± 0.232
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski