Sphingomonas sp. FARSPH

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; unclassified Sphingomonas

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3408 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345WLE7|A0A345WLE7_9SPHN CopG family transcriptional regulator OS=Sphingomonas sp. FARSPH OX=2219696 GN=DM480_02345 PE=4 SV=1
MM1 pKa = 7.41TDD3 pKa = 3.15AAEE6 pKa = 4.37SNPRR10 pKa = 11.84IYY12 pKa = 10.29VACLAAYY19 pKa = 10.35NNGYY23 pKa = 9.03LHH25 pKa = 6.64GAWIDD30 pKa = 3.46ADD32 pKa = 3.48QDD34 pKa = 3.29ADD36 pKa = 4.25EE37 pKa = 4.78IRR39 pKa = 11.84SEE41 pKa = 3.88IAAMLSRR48 pKa = 11.84SPIEE52 pKa = 4.05GAEE55 pKa = 4.02EE56 pKa = 4.06YY57 pKa = 10.53AIHH60 pKa = 7.57DD61 pKa = 3.89YY62 pKa = 11.51EE63 pKa = 4.61GFEE66 pKa = 4.05GVAIEE71 pKa = 4.19EE72 pKa = 4.18FAGIEE77 pKa = 3.95NVARR81 pKa = 11.84IAAFVAEE88 pKa = 4.86HH89 pKa = 6.38GALGAGLLNEE99 pKa = 4.36FVGDD103 pKa = 3.38IDD105 pKa = 3.87QAEE108 pKa = 4.2TAMRR112 pKa = 11.84DD113 pKa = 3.77CYY115 pKa = 10.59HH116 pKa = 6.39GQFTSLADD124 pKa = 3.6YY125 pKa = 10.44MEE127 pKa = 5.11EE128 pKa = 4.0LTADD132 pKa = 3.53TVTIPDD138 pKa = 3.66ALRR141 pKa = 11.84YY142 pKa = 9.57YY143 pKa = 10.25IDD145 pKa = 3.54WQAMARR151 pKa = 11.84DD152 pKa = 3.99AEE154 pKa = 4.37LGGDD158 pKa = 4.28LFTVEE163 pKa = 3.76TAYY166 pKa = 10.96DD167 pKa = 3.77EE168 pKa = 4.3VHH170 pKa = 5.62VFSRR174 pKa = 11.84RR175 pKa = 3.3

Molecular weight: 19.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345WT58|A0A345WT58_9SPHN Putative pterin-4-alpha-carbinolamine dehydratase OS=Sphingomonas sp. FARSPH OX=2219696 GN=DM480_05330 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84AVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3408

0

3408

1101842

29

4693

323.3

34.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.595 ± 0.079

0.709 ± 0.012

6.145 ± 0.036

4.605 ± 0.044

3.325 ± 0.021

9.078 ± 0.047

1.967 ± 0.024

4.739 ± 0.028

2.537 ± 0.033

9.784 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.302 ± 0.023

2.291 ± 0.031

5.513 ± 0.03

3.043 ± 0.03

7.867 ± 0.051

4.689 ± 0.035

5.705 ± 0.06

7.483 ± 0.032

1.414 ± 0.021

2.209 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski