Candidatus Purcelliella pentastirinorum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Enterobacteriaceae incertae sedis; ant, tsetse, mealybug, aphid, etc. endosymbionts; Candidatus Purcelliella

Average proteome isoelectric point is 8.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 427 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A346E091|A0A346E091_9ENTR Ribosomal RNA large subunit methyltransferase E OS=Candidatus Purcelliella pentastirinorum OX=472834 GN=C9I82_450 PE=4 SV=1
MM1 pKa = 7.38NKK3 pKa = 9.81IIFDD7 pKa = 3.91TDD9 pKa = 2.76IGIDD13 pKa = 3.55DD14 pKa = 4.68AFALLLAYY22 pKa = 7.58KK23 pKa = 8.02TQNILGITTVFGNTNINQVTNNAVLFSKK51 pKa = 10.61KK52 pKa = 9.82FDD54 pKa = 3.5IDD56 pKa = 3.58VPIYY60 pKa = 10.05KK61 pKa = 10.18GCSNSLVYY69 pKa = 10.92NNSFDD74 pKa = 4.53DD75 pKa = 4.48SINIHH80 pKa = 6.1GNNGLGDD87 pKa = 3.73IFNDD91 pKa = 3.3YY92 pKa = 10.67VNYY95 pKa = 9.89VAYY98 pKa = 10.12DD99 pKa = 3.47ALEE102 pKa = 4.49FIIDD106 pKa = 4.25SINDD110 pKa = 3.45NPHH113 pKa = 6.39EE114 pKa = 5.13IILVALGPLTNIATVIKK131 pKa = 9.96KK132 pKa = 10.44CPEE135 pKa = 3.95LVSKK139 pKa = 10.69IKK141 pKa = 10.44FLIIMGGAFGTDD153 pKa = 2.88GCFGNITCNSEE164 pKa = 3.87FNVWSDD170 pKa = 3.28PHH172 pKa = 6.22AAQLIFLSKK181 pKa = 10.67LPIVVVPLDD190 pKa = 3.66VTHH193 pKa = 7.1KK194 pKa = 10.64IVITEE199 pKa = 4.46DD200 pKa = 3.64DD201 pKa = 4.51LLSLNNNFLIDD212 pKa = 4.04LSRR215 pKa = 11.84CYY217 pKa = 10.42MSFYY221 pKa = 10.23RR222 pKa = 11.84NKK224 pKa = 10.37KK225 pKa = 9.6FFDD228 pKa = 3.46GMVLHH233 pKa = 7.05DD234 pKa = 5.79PIVISYY240 pKa = 10.22LIEE243 pKa = 4.96DD244 pKa = 3.22KK245 pKa = 10.88WFDD248 pKa = 3.59VKK250 pKa = 10.91DD251 pKa = 3.74VYY253 pKa = 11.67VEE255 pKa = 4.03VLTDD259 pKa = 3.24SLYY262 pKa = 10.72RR263 pKa = 11.84GSTLMYY269 pKa = 9.55DD270 pKa = 3.8KK271 pKa = 10.8KK272 pKa = 11.29YY273 pKa = 10.53DD274 pKa = 3.41FHH276 pKa = 8.32NILHH280 pKa = 6.73HH281 pKa = 6.74NDD283 pKa = 2.79SLLRR287 pKa = 11.84KK288 pKa = 9.3VCLNLDD294 pKa = 3.77VVNVKK299 pKa = 10.79SNLLSILKK307 pKa = 9.87II308 pKa = 3.86

Molecular weight: 34.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A346DZI5|A0A346DZI5_9ENTR 3'(2') 5'-bisphosphate nucleotidase CysQ OS=Candidatus Purcelliella pentastirinorum OX=472834 GN=cysQ PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.58RR3 pKa = 11.84TFQPSLIKK11 pKa = 10.35RR12 pKa = 11.84SRR14 pKa = 11.84LHH16 pKa = 6.02GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 9.97NGRR28 pKa = 11.84NILARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84FKK37 pKa = 11.09GRR39 pKa = 11.84IRR41 pKa = 11.84LTVLWW46 pKa = 4.29

Molecular weight: 5.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

427

0

427

139146

38

1428

325.9

37.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.525 ± 0.114

1.195 ± 0.041

3.991 ± 0.084

4.182 ± 0.119

5.341 ± 0.17

5.113 ± 0.116

1.634 ± 0.039

14.189 ± 0.195

11.729 ± 0.264

9.818 ± 0.131

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.163 ± 0.048

8.901 ± 0.17

2.62 ± 0.053

2.107 ± 0.058

3.32 ± 0.094

6.33 ± 0.101

4.13 ± 0.08

4.174 ± 0.123

0.908 ± 0.041

4.628 ± 0.091
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski