Cohnella abietis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Cohnella

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6200 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3T1DBF8|A0A3T1DBF8_9BACL Uncharacterized protein OS=Cohnella abietis OX=2507935 GN=KCTCHS21_48280 PE=4 SV=1
MM1 pKa = 7.39FLGNVAVKK9 pKa = 10.01VANCDD14 pKa = 3.58DD15 pKa = 3.78GEE17 pKa = 4.42EE18 pKa = 4.12IHH20 pKa = 7.66GEE22 pKa = 4.02EE23 pKa = 4.0AVKK26 pKa = 10.73VAICDD31 pKa = 3.43NGGVFLRR38 pKa = 11.84DD39 pKa = 3.25GAIKK43 pKa = 10.58VAFCDD48 pKa = 4.38DD49 pKa = 3.69GEE51 pKa = 4.27EE52 pKa = 4.0FLGYY56 pKa = 10.37GAIKK60 pKa = 10.44VAICDD65 pKa = 3.87DD66 pKa = 3.9GEE68 pKa = 4.22EE69 pKa = 4.17FLGNDD74 pKa = 3.89AIKK77 pKa = 10.81VAFCDD82 pKa = 5.1DD83 pKa = 3.42GDD85 pKa = 3.73EE86 pKa = 4.13FLGYY90 pKa = 10.42GAIKK94 pKa = 10.44VAICDD99 pKa = 3.73DD100 pKa = 4.25GEE102 pKa = 4.44VFLGDD107 pKa = 4.62DD108 pKa = 3.39ATKK111 pKa = 10.67VANCDD116 pKa = 3.56DD117 pKa = 4.49GEE119 pKa = 4.25VLLGGGAVKK128 pKa = 10.41VAICDD133 pKa = 3.75DD134 pKa = 4.19GEE136 pKa = 4.49VLVDD140 pKa = 3.58EE141 pKa = 4.94

Molecular weight: 14.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3T1CXS8|A0A3T1CXS8_9BACL Putative metal-dependent hydrolase YabD OS=Cohnella abietis OX=2507935 GN=yabD PE=4 SV=1
MM1 pKa = 7.61GPTFKK6 pKa = 10.87PNVSKK11 pKa = 10.8RR12 pKa = 11.84KK13 pKa = 8.57KK14 pKa = 8.17VHH16 pKa = 5.5GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTKK25 pKa = 9.61NGRR28 pKa = 11.84RR29 pKa = 11.84VLSARR34 pKa = 11.84RR35 pKa = 11.84HH36 pKa = 5.5KK37 pKa = 10.39GRR39 pKa = 11.84KK40 pKa = 8.89VLTAA44 pKa = 4.27

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6200

0

6200

2032609

39

2724

327.8

36.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.779 ± 0.037

0.716 ± 0.011

5.19 ± 0.02

6.463 ± 0.036

4.184 ± 0.025

7.241 ± 0.033

1.98 ± 0.016

7.121 ± 0.03

5.607 ± 0.025

9.964 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.641 ± 0.015

4.331 ± 0.025

3.935 ± 0.02

3.739 ± 0.019

4.675 ± 0.034

6.821 ± 0.029

5.667 ± 0.038

7.059 ± 0.028

1.348 ± 0.014

3.538 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski