Staphylococcus prophage phiPV83

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bronfenbrennervirinae; Peeveelvirus; Staphylococcus virus PV83

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9MBR2|Q9MBR2_9CAUD Uncharacterized protein OS=Staphylococcus prophage phiPV83 OX=129009 PE=4 SV=1
MM1 pKa = 8.14DD2 pKa = 3.98NRR4 pKa = 11.84EE5 pKa = 4.06QIEE8 pKa = 3.92QSVISASAYY17 pKa = 10.04NGNDD21 pKa = 3.29TEE23 pKa = 4.52GLLKK27 pKa = 10.49EE28 pKa = 4.44IEE30 pKa = 4.24DD31 pKa = 4.28VYY33 pKa = 11.57KK34 pKa = 10.64KK35 pKa = 10.72AQAFDD40 pKa = 4.61EE41 pKa = 4.66ILDD44 pKa = 3.9GMTNAIQHH52 pKa = 5.29SVKK55 pKa = 10.58EE56 pKa = 4.41GIEE59 pKa = 3.75LDD61 pKa = 3.69EE62 pKa = 4.94AVGIMAGQVVYY73 pKa = 10.26KK74 pKa = 10.69YY75 pKa = 10.76EE76 pKa = 3.97EE77 pKa = 4.06EE78 pKa = 4.28QEE80 pKa = 3.93NDD82 pKa = 2.92

Molecular weight: 9.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9MBN0|Q9MBN0_9CAUD Uncharacterized protein OS=Staphylococcus prophage phiPV83 OX=129009 PE=4 SV=1
MM1 pKa = 7.86LITKK5 pKa = 7.85KK6 pKa = 8.26THH8 pKa = 4.53TRR10 pKa = 11.84SANMSYY16 pKa = 10.72NHH18 pKa = 6.39LTLTEE23 pKa = 3.88RR24 pKa = 11.84ARR26 pKa = 11.84IEE28 pKa = 3.93VLRR31 pKa = 11.84QEE33 pKa = 4.41NYY35 pKa = 9.79SLRR38 pKa = 11.84SIARR42 pKa = 11.84KK43 pKa = 9.56LKK45 pKa = 10.38RR46 pKa = 11.84SVSTISRR53 pKa = 11.84EE54 pKa = 3.6ISRR57 pKa = 11.84NNLNQSYY64 pKa = 8.93QAEE67 pKa = 4.38TAQKK71 pKa = 10.52NYY73 pKa = 5.05EE74 pKa = 4.34TKK76 pKa = 10.47RR77 pKa = 11.84KK78 pKa = 9.76LCGRR82 pKa = 11.84PTRR85 pKa = 11.84FTPEE89 pKa = 3.51LGNIIKK95 pKa = 10.66YY96 pKa = 8.52YY97 pKa = 10.25LKK99 pKa = 10.53CHH101 pKa = 6.78WSPEE105 pKa = 4.22QIVGRR110 pKa = 11.84LLQNQICFKK119 pKa = 10.49TIYY122 pKa = 10.1RR123 pKa = 11.84WINSNMINFEE133 pKa = 4.82LISCLRR139 pKa = 11.84QKK141 pKa = 10.75GKK143 pKa = 9.53RR144 pKa = 11.84QKK146 pKa = 10.36PKK148 pKa = 7.99EE149 pKa = 4.12TRR151 pKa = 11.84GKK153 pKa = 10.44FNIGRR158 pKa = 11.84PISQRR163 pKa = 11.84PKK165 pKa = 9.92EE166 pKa = 4.0IKK168 pKa = 9.68KK169 pKa = 10.62RR170 pKa = 11.84NTFGHH175 pKa = 6.53WEE177 pKa = 3.99ADD179 pKa = 3.84TIVSSRR185 pKa = 11.84GKK187 pKa = 10.46SKK189 pKa = 11.02GCIATFAEE197 pKa = 4.52RR198 pKa = 11.84KK199 pKa = 8.75SRR201 pKa = 11.84YY202 pKa = 8.36YY203 pKa = 10.97YY204 pKa = 10.34CVLMPDD210 pKa = 3.59RR211 pKa = 11.84SSNSMEE217 pKa = 3.78TAINNLIKK225 pKa = 10.34HH226 pKa = 5.96LPKK229 pKa = 10.68GAVKK233 pKa = 9.83TITVDD238 pKa = 2.86RR239 pKa = 11.84GKK241 pKa = 10.78EE242 pKa = 3.82FSCYY246 pKa = 8.19QNIEE250 pKa = 3.79NQFNINVYY258 pKa = 10.08FADD261 pKa = 5.7PYY263 pKa = 10.8SAWQRR268 pKa = 11.84GTNEE272 pKa = 3.82NTNGLLRR279 pKa = 11.84EE280 pKa = 4.35FFPKK284 pKa = 9.46KK285 pKa = 8.99TDD287 pKa = 3.57LAKK290 pKa = 10.89VNQEE294 pKa = 3.89QLNYY298 pKa = 10.91ALDD301 pKa = 4.39SINYY305 pKa = 9.43RR306 pKa = 11.84PRR308 pKa = 11.84KK309 pKa = 7.71CLNWKK314 pKa = 9.79FPYY317 pKa = 10.02EE318 pKa = 4.13VLCDD322 pKa = 3.89EE323 pKa = 5.09LLHH326 pKa = 7.02LNN328 pKa = 4.0

Molecular weight: 38.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

12979

46

1313

206.0

23.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.178 ± 0.385

0.578 ± 0.129

6.31 ± 0.318

7.373 ± 0.448

4.176 ± 0.268

5.393 ± 0.574

1.641 ± 0.132

7.766 ± 0.183

9.292 ± 0.321

8.152 ± 0.322

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.65 ± 0.214

6.988 ± 0.287

2.666 ± 0.152

4.045 ± 0.224

4.692 ± 0.326

5.987 ± 0.276

5.732 ± 0.223

5.779 ± 0.276

1.194 ± 0.134

4.407 ± 0.323
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski