Streptococcus phage phi20c

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1Q0V4|M1Q0V4_9CAUD Uncharacterized protein OS=Streptococcus phage phi20c OX=1289595 GN=phi20c_0011 PE=4 SV=1
MM1 pKa = 7.1NQDD4 pKa = 3.91QITQALRR11 pKa = 11.84LTNNDD16 pKa = 3.05LVAKK20 pKa = 10.09LSEE23 pKa = 4.05EE24 pKa = 4.04MTTKK28 pKa = 10.77NLLAIQLTEE37 pKa = 3.87AQQIIANLQAEE48 pKa = 4.65IADD51 pKa = 3.88LTQQLDD57 pKa = 3.67EE58 pKa = 5.02AIKK61 pKa = 10.28PEE63 pKa = 4.21EE64 pKa = 5.08IIDD67 pKa = 3.94QEE69 pKa = 4.17EE70 pKa = 4.75GEE72 pKa = 4.25

Molecular weight: 8.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1PF64|M1PF64_9CAUD Putative bifunctional DNA primase/polymerase OS=Streptococcus phage phi20c OX=1289595 GN=phi20c_0033 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.8PIRR5 pKa = 11.84YY6 pKa = 8.14PYY8 pKa = 9.79SGKK11 pKa = 8.65TKK13 pKa = 10.03IVRR16 pKa = 11.84KK17 pKa = 9.26EE18 pKa = 3.61MPRR21 pKa = 11.84FIMLSYY27 pKa = 9.88TAFDD31 pKa = 3.51SRR33 pKa = 11.84LVDD36 pKa = 4.93HH37 pKa = 7.58IDD39 pKa = 3.36TMVQTGMSEE48 pKa = 3.94TLITFKK54 pKa = 10.48IPRR57 pKa = 11.84LFSYY61 pKa = 9.67EE62 pKa = 3.99EE63 pKa = 3.78KK64 pKa = 10.42QIRR67 pKa = 11.84VPLPLIKK74 pKa = 10.24VVKK77 pKa = 10.24ILNQYY82 pKa = 9.14

Molecular weight: 9.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

10489

32

1231

194.2

21.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.703 ± 0.492

0.667 ± 0.099

6.083 ± 0.284

7.351 ± 0.479

4.109 ± 0.266

6.426 ± 0.538

1.421 ± 0.162

6.769 ± 0.309

7.97 ± 0.512

8.037 ± 0.259

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.641 ± 0.244

5.444 ± 0.265

2.879 ± 0.287

4.176 ± 0.266

4.776 ± 0.318

6.397 ± 0.424

5.959 ± 0.4

6.559 ± 0.276

1.192 ± 0.112

3.442 ± 0.23
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski