Helicobacter bilis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Epsilonproteobacteria; Campylobacterales; Helicobacteraceae; Helicobacter

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2030 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A099V2X6|A0A099V2X6_9HELI Uncharacterized protein OS=Helicobacter bilis OX=37372 GN=LS79_010890 PE=4 SV=1
MM1 pKa = 7.6QYY3 pKa = 10.27PISDD7 pKa = 3.72TGHH10 pKa = 5.31VLCYY14 pKa = 10.19LYY16 pKa = 10.76YY17 pKa = 10.54AEE19 pKa = 5.62KK20 pKa = 10.59LYY22 pKa = 11.11DD23 pKa = 3.85EE24 pKa = 4.57NEE26 pKa = 4.23KK27 pKa = 10.79EE28 pKa = 3.67ISEE31 pKa = 4.36YY32 pKa = 10.75FDD34 pKa = 3.67NLCNEE39 pKa = 4.78CDD41 pKa = 3.35TSGTDD46 pKa = 3.92FIKK49 pKa = 10.55MSVTEE54 pKa = 4.23ILNSPSIAKK63 pKa = 9.62NRR65 pKa = 11.84IIEE68 pKa = 4.82GYY70 pKa = 9.36LQSLANEE77 pKa = 3.99LMDD80 pKa = 4.81YY81 pKa = 10.3EE82 pKa = 4.26ISEE85 pKa = 4.65NDD87 pKa = 4.78LEE89 pKa = 4.49SQDD92 pKa = 4.14SKK94 pKa = 10.47TEE96 pKa = 3.75

Molecular weight: 11.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4U8U8X0|A0A4U8U8X0_9HELI Carbam_trans_N domain-containing protein OS=Helicobacter bilis OX=37372 GN=LS78_005100 PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.02QPHH8 pKa = 5.69DD9 pKa = 3.52TPRR12 pKa = 11.84KK13 pKa = 7.03RR14 pKa = 11.84THH16 pKa = 6.26GFRR19 pKa = 11.84TRR21 pKa = 11.84MKK23 pKa = 8.5TKK25 pKa = 9.96NGRR28 pKa = 11.84RR29 pKa = 11.84VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.41GRR39 pKa = 11.84KK40 pKa = 8.41RR41 pKa = 11.84LSVAA45 pKa = 3.33

Molecular weight: 5.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2030

0

2030

625050

31

2883

307.9

34.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.737 ± 0.051

1.331 ± 0.024

5.352 ± 0.038

6.32 ± 0.057

4.975 ± 0.054

5.709 ± 0.062

2.175 ± 0.024

8.399 ± 0.048

8.069 ± 0.057

9.944 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.599 ± 0.025

5.904 ± 0.052

2.967 ± 0.028

3.939 ± 0.039

3.553 ± 0.032

6.957 ± 0.044

5.076 ± 0.039

5.253 ± 0.05

0.69 ± 0.017

4.053 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski