Salmonella phage SP069

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Nonanavirus; Salmonella virus SP069

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|S4TVY0|S4TVY0_9CAUD Uncharacterized protein OS=Salmonella phage SP069 OX=1173760 GN=SP069_00080 PE=4 SV=1
MM1 pKa = 7.3AQVKK5 pKa = 9.7CIKK8 pKa = 10.51DD9 pKa = 3.44NEE11 pKa = 4.88GYY13 pKa = 6.92WTEE16 pKa = 4.19GEE18 pKa = 4.5VYY20 pKa = 9.9HH21 pKa = 7.18AEE23 pKa = 4.1SAPGGFLNVGDD34 pKa = 5.57DD35 pKa = 3.86DD36 pKa = 5.09DD37 pKa = 6.44KK38 pKa = 11.25NAEE41 pKa = 3.9WSLMPVSYY49 pKa = 10.91DD50 pKa = 3.88DD51 pKa = 3.76NDD53 pKa = 3.49KK54 pKa = 11.22AIYY57 pKa = 9.88QLTGLDD63 pKa = 3.35AQFIDD68 pKa = 3.84VV69 pKa = 4.08

Molecular weight:
7.7 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|S4TU54|S4TU54_9CAUD Uncharacterized protein OS=Salmonella phage SP069 OX=1173760 GN=SP069_00045 PE=4 SV=1
MM1 pKa = 7.47IKK3 pKa = 10.45VGLSPGASYY12 pKa = 10.78AKK14 pKa = 10.48QMIEE18 pKa = 3.77QLKK21 pKa = 10.45SLGEE25 pKa = 3.9LTVEE29 pKa = 4.91DD30 pKa = 4.44FALFDD35 pKa = 5.41GIPQVTQQLPTKK47 pKa = 9.32QPRR50 pKa = 11.84RR51 pKa = 11.84KK52 pKa = 8.44TGVAQARR59 pKa = 11.84HH60 pKa = 5.15NARR63 pKa = 11.84KK64 pKa = 9.55RR65 pKa = 11.84RR66 pKa = 11.84NRR68 pKa = 11.84KK69 pKa = 8.72

Molecular weight:
7.79 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

12341

58

971

192.8

21.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.225 ± 0.687

1.045 ± 0.158

6.418 ± 0.241

6.061 ± 0.303

3.476 ± 0.198

7.26 ± 0.438

1.783 ± 0.291

6.004 ± 0.275

5.543 ± 0.307

7.828 ± 0.264

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.617 ± 0.213

5.332 ± 0.283

4.1 ± 0.272

4.457 ± 0.665

4.959 ± 0.282

5.826 ± 0.271

6.685 ± 0.387

7.131 ± 0.306

1.58 ± 0.167

3.671 ± 0.268

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski