Chlorogloeopsis fritschii PCC 6912

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Chlorogloeopsidaceae; Chlorogloeopsis; Chlorogloeopsis fritschii

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6594 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S1A462|A0A3S1A462_CHLFR CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase OS=Chlorogloeopsis fritschii PCC 6912 OX=211165 GN=PCC6912_32120 PE=3 SV=1
MM1 pKa = 7.34FSSSFPDD8 pKa = 3.36EE9 pKa = 4.54HH10 pKa = 8.19DD11 pKa = 4.26HH12 pKa = 5.77EE13 pKa = 5.65HH14 pKa = 6.65SGTISLTDD22 pKa = 3.4DD23 pKa = 3.08KK24 pKa = 11.39GRR26 pKa = 11.84SLEE29 pKa = 4.17CYY31 pKa = 9.3IEE33 pKa = 4.46HH34 pKa = 6.36SLEE37 pKa = 4.04VDD39 pKa = 3.21GQEE42 pKa = 4.18YY43 pKa = 10.45FLLLPVDD50 pKa = 4.04SPVEE54 pKa = 3.75IFAWQGDD61 pKa = 4.15GEE63 pKa = 4.36EE64 pKa = 4.42EE65 pKa = 3.86EE66 pKa = 5.19AILVEE71 pKa = 5.13DD72 pKa = 5.35DD73 pKa = 3.45ATIDD77 pKa = 4.19QIFTTAQAVLSEE89 pKa = 4.2QNLILKK95 pKa = 7.58NTAYY99 pKa = 10.66ALTVSGEE106 pKa = 4.08LPPVEE111 pKa = 4.19EE112 pKa = 4.78SEE114 pKa = 4.93LFTLEE119 pKa = 4.49IEE121 pKa = 4.77DD122 pKa = 4.57EE123 pKa = 4.36EE124 pKa = 5.86SDD126 pKa = 4.95LEE128 pKa = 4.23PEE130 pKa = 4.08QLQLLSSFYY139 pKa = 11.07YY140 pKa = 10.36EE141 pKa = 3.92EE142 pKa = 3.94QEE144 pKa = 4.27YY145 pKa = 10.72AIYY148 pKa = 9.85TPLDD152 pKa = 3.6PLLFFARR159 pKa = 11.84ISKK162 pKa = 10.01SGKK165 pKa = 9.24PEE167 pKa = 3.95LLSPEE172 pKa = 4.69EE173 pKa = 3.95FRR175 pKa = 11.84QVQPLLEE182 pKa = 4.13EE183 pKa = 3.84QLFNEE188 pKa = 4.71VEE190 pKa = 3.98

Molecular weight: 21.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S0ZZZ1|A0A3S0ZZZ1_CHLFR Photosystem II 12 kDa extrinsic protein OS=Chlorogloeopsis fritschii PCC 6912 OX=211165 GN=psbU PE=3 SV=1
MM1 pKa = 6.96TTHH4 pKa = 6.98CSNWIVGIDD13 pKa = 3.64AQHH16 pKa = 6.28GNPYY20 pKa = 10.61DD21 pKa = 4.04GATLAPALLQVEE33 pKa = 4.82NLTHH37 pKa = 5.63VQPQQAIVDD46 pKa = 3.52QGFRR50 pKa = 11.84GSTHH54 pKa = 6.58HH55 pKa = 6.34PQAVQVLIAGNRR67 pKa = 11.84KK68 pKa = 8.99RR69 pKa = 11.84AGFLKK74 pKa = 10.44RR75 pKa = 11.84LLKK78 pKa = 10.24RR79 pKa = 11.84RR80 pKa = 11.84SAIEE84 pKa = 3.54PVIGHH89 pKa = 6.28TKK91 pKa = 9.64HH92 pKa = 6.02DD93 pKa = 3.21HH94 pKa = 5.62RR95 pKa = 11.84MQRR98 pKa = 11.84NYY100 pKa = 11.08LQGQVGDD107 pKa = 4.83RR108 pKa = 11.84INSFLAGCGFNLRR121 pKa = 11.84KK122 pKa = 10.01LYY124 pKa = 10.67RR125 pKa = 11.84FFVTAPLIHH134 pKa = 6.75LVAA137 pKa = 5.67

Molecular weight: 15.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6594

0

6594

2055004

43

4184

311.6

34.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.272 ± 0.033

1.029 ± 0.011

4.672 ± 0.023

6.375 ± 0.034

4.023 ± 0.02

6.535 ± 0.029

1.889 ± 0.016

6.784 ± 0.024

4.88 ± 0.029

10.929 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.898 ± 0.013

4.315 ± 0.023

4.68 ± 0.024

5.396 ± 0.028

5.207 ± 0.027

6.176 ± 0.024

5.603 ± 0.027

6.762 ± 0.022

1.453 ± 0.014

3.111 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski