Arthrobacter phage Shepard

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Gordonvirus; unclassified Gordonvirus

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 92 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6TUT9|A0A5J6TUT9_9CAUD Uncharacterized protein OS=Arthrobacter phage Shepard OX=2599833 GN=38 PE=4 SV=1
MM1 pKa = 7.49TKK3 pKa = 10.38LAWDD7 pKa = 3.56AVGEE11 pKa = 4.25RR12 pKa = 11.84FFEE15 pKa = 4.56AGADD19 pKa = 3.55RR20 pKa = 11.84GVLYY24 pKa = 10.61LDD26 pKa = 5.28DD27 pKa = 5.7VGHH30 pKa = 6.92PWNGLVAVEE39 pKa = 4.22QSPSGGEE46 pKa = 3.82PEE48 pKa = 4.84TYY50 pKa = 10.31YY51 pKa = 10.89QDD53 pKa = 3.56GLPYY57 pKa = 9.95ISVSASEE64 pKa = 4.22EE65 pKa = 4.0FKK67 pKa = 11.3GSIEE71 pKa = 4.41AYY73 pKa = 8.28TYY75 pKa = 9.79PNAFAVCDD83 pKa = 4.23GSSEE87 pKa = 4.2IAQGMYY93 pKa = 10.63LSQQDD98 pKa = 3.7RR99 pKa = 11.84KK100 pKa = 10.39PFGLSYY106 pKa = 9.84RR107 pKa = 11.84TLIGNDD113 pKa = 2.96IQEE116 pKa = 4.58LEE118 pKa = 4.32FGYY121 pKa = 10.14KK122 pKa = 8.87IHH124 pKa = 7.73IIYY127 pKa = 10.62NCMASPSSRR136 pKa = 11.84SYY138 pKa = 11.47SSLGEE143 pKa = 3.9SDD145 pKa = 3.4NAEE148 pKa = 4.04TFNWSITTRR157 pKa = 11.84PVKK160 pKa = 10.76FEE162 pKa = 4.5DD163 pKa = 3.22VTFGIKK169 pKa = 10.21YY170 pKa = 8.8GAHH173 pKa = 4.98VTLDD177 pKa = 3.4SRR179 pKa = 11.84EE180 pKa = 4.09VYY182 pKa = 8.57PWAMAAVEE190 pKa = 4.1EE191 pKa = 4.42VLYY194 pKa = 8.91GTDD197 pKa = 3.12EE198 pKa = 4.32TEE200 pKa = 4.09ARR202 pKa = 11.84LPTPQEE208 pKa = 3.92LLDD211 pKa = 4.24LFVDD215 pKa = 3.7NALLKK220 pKa = 9.83ITDD223 pKa = 4.11NGDD226 pKa = 3.64GTWTAEE232 pKa = 4.16APDD235 pKa = 4.92SIISMLSDD243 pKa = 4.61EE244 pKa = 4.52IFQINWEE251 pKa = 4.3SAVMLDD257 pKa = 3.36EE258 pKa = 4.34DD259 pKa = 5.0TYY261 pKa = 10.86TISSFF266 pKa = 3.37

Molecular weight: 29.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6TS94|A0A5J6TS94_9CAUD Helix-turn-helix DNA-binding protein OS=Arthrobacter phage Shepard OX=2599833 GN=91 PE=4 SV=1
MM1 pKa = 7.56GAGLKK6 pKa = 8.59PTFKK10 pKa = 10.15PACPKK15 pKa = 10.48CDD17 pKa = 3.53FTLGYY22 pKa = 9.07YY23 pKa = 10.1VRR25 pKa = 11.84DD26 pKa = 3.91PDD28 pKa = 3.66VHH30 pKa = 6.13PDD32 pKa = 2.88KK33 pKa = 10.69WVLNEE38 pKa = 4.86ASRR41 pKa = 11.84QHH43 pKa = 6.31LKK45 pKa = 9.12EE46 pKa = 3.53AHH48 pKa = 6.21VIVVPEE54 pKa = 3.85VRR56 pKa = 11.84AIHH59 pKa = 5.87PVRR62 pKa = 11.84SFRR65 pKa = 11.84SRR67 pKa = 11.84RR68 pKa = 11.84RR69 pKa = 11.84LLSSKK74 pKa = 10.28RR75 pKa = 11.84RR76 pKa = 11.84VLVV79 pKa = 3.36

Molecular weight: 9.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

92

0

92

18233

30

1529

198.2

22.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.814 ± 0.519

0.592 ± 0.103

6.049 ± 0.348

6.554 ± 0.388

3.73 ± 0.205

7.525 ± 0.506

1.892 ± 0.182

6.017 ± 0.219

6.609 ± 0.418

7.848 ± 0.394

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.808 ± 0.256

4.448 ± 0.186

4.201 ± 0.252

3.472 ± 0.163

5.298 ± 0.338

6.198 ± 0.257

6.368 ± 0.282

6.62 ± 0.2

1.585 ± 0.159

3.373 ± 0.283
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski