Oxynema sp. AP17

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Oscillatoriophycideae; Oscillatoriales; Microcoleaceae; Oxynema; unclassified Oxynema

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4779 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6H1U401|A0A6H1U401_9CYAN Deoxyribodipyrimidine photolyase OS=Oxynema sp. AP17 OX=2064643 GN=HCG48_18645 PE=3 SV=1
MM1 pKa = 7.44EE2 pKa = 5.17NWPTDD7 pKa = 3.59LFKK10 pKa = 11.18LLEE13 pKa = 4.43SATEE17 pKa = 3.88DD18 pKa = 3.32VEE20 pKa = 6.11GFFQEE25 pKa = 4.55LSEE28 pKa = 4.19EE29 pKa = 4.25VNEE32 pKa = 5.21FFDD35 pKa = 5.11DD36 pKa = 3.78LAKK39 pKa = 10.41IPEE42 pKa = 4.29QITRR46 pKa = 11.84DD47 pKa = 3.84FNDD50 pKa = 3.63GLDD53 pKa = 3.52LVEE56 pKa = 4.34EE57 pKa = 4.36LEE59 pKa = 4.19EE60 pKa = 4.88GFFLEE65 pKa = 3.74IDD67 pKa = 3.54RR68 pKa = 11.84FFLEE72 pKa = 4.59VFDD75 pKa = 4.09PTLAEE80 pKa = 3.86EE81 pKa = 4.56LNRR84 pKa = 11.84FDD86 pKa = 6.56LEE88 pKa = 4.37DD89 pKa = 3.79LFYY92 pKa = 11.25DD93 pKa = 4.05LDD95 pKa = 4.39RR96 pKa = 11.84SVDD99 pKa = 3.82EE100 pKa = 4.98NSPDD104 pKa = 3.51PPQPFVTYY112 pKa = 10.08VRR114 pKa = 11.84PTQGRR119 pKa = 11.84HH120 pKa = 4.59PACVGCYY127 pKa = 8.86HH128 pKa = 5.67YY129 pKa = 10.56HH130 pKa = 4.67GHH132 pKa = 6.12VYY134 pKa = 10.46GGNLLVCGMHH144 pKa = 6.98PYY146 pKa = 10.47GWDD149 pKa = 3.61GEE151 pKa = 4.61SCPDD155 pKa = 3.28WEE157 pKa = 5.09SEE159 pKa = 4.22VV160 pKa = 3.53

Molecular weight: 18.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6H1TX51|A0A6H1TX51_9CYAN ABC transporter ATP-binding protein OS=Oxynema sp. AP17 OX=2064643 GN=HCG48_11785 PE=4 SV=1
MM1 pKa = 6.63TQRR4 pKa = 11.84TLHH7 pKa = 4.82GTSRR11 pKa = 11.84KK12 pKa = 8.94RR13 pKa = 11.84KK14 pKa = 7.72RR15 pKa = 11.84VSGFRR20 pKa = 11.84VRR22 pKa = 11.84MRR24 pKa = 11.84TKK26 pKa = 10.53NGQKK30 pKa = 9.45TIAARR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.11KK38 pKa = 10.03GRR40 pKa = 11.84HH41 pKa = 5.0RR42 pKa = 11.84LSVV45 pKa = 3.12

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4779

0

4779

1694947

22

2913

354.7

39.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.966 ± 0.036

1.008 ± 0.011

5.64 ± 0.032

6.86 ± 0.035

3.796 ± 0.026

7.181 ± 0.043

1.808 ± 0.015

6.049 ± 0.023

3.727 ± 0.032

10.879 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.648 ± 0.016

3.611 ± 0.03

5.149 ± 0.033

4.721 ± 0.028

6.638 ± 0.038

6.243 ± 0.032

5.368 ± 0.031

6.373 ± 0.029

1.502 ± 0.017

2.831 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski