African swine fever virus (isolate Warthog/Namibia/Wart80/1980) (ASFV)

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes; Asfuvirales; Asfarviridae; Asfivirus; African swine fever virus

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 159 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P0C8H6|ARBH_ASFWA Apoptosis regulator Bcl-2 homolog OS=African swine fever virus (isolate Warthog/Namibia/Wart80/1980) OX=561444 GN=War-051 PE=3 SV=1
MM1 pKa = 7.5VSRR4 pKa = 11.84FLMAEE9 pKa = 3.82YY10 pKa = 10.29RR11 pKa = 11.84HH12 pKa = 6.46LIEE15 pKa = 5.41NPSEE19 pKa = 3.97NFKK22 pKa = 10.91ISVNEE27 pKa = 3.78NDD29 pKa = 3.14MTEE32 pKa = 3.59WDD34 pKa = 4.09VILRR38 pKa = 11.84GPPDD42 pKa = 3.39TLYY45 pKa = 11.18EE46 pKa = 4.56GGLFKK51 pKa = 11.06AKK53 pKa = 10.14VAFPPEE59 pKa = 4.18YY60 pKa = 9.73PYY62 pKa = 11.32APPRR66 pKa = 11.84LTFTSEE72 pKa = 3.2MWHH75 pKa = 6.31PNIYY79 pKa = 9.86PDD81 pKa = 3.75GKK83 pKa = 10.03LCISILHH90 pKa = 6.67GDD92 pKa = 3.56NAEE95 pKa = 4.12EE96 pKa = 5.24QGMTWSPAQKK106 pKa = 9.64IDD108 pKa = 3.88TILLSVISLLNEE120 pKa = 4.11PNPDD124 pKa = 3.04SPANVDD130 pKa = 3.14AAKK133 pKa = 10.26SYY135 pKa = 10.69RR136 pKa = 11.84KK137 pKa = 9.82LMHH140 pKa = 6.81KK141 pKa = 10.11EE142 pKa = 3.84DD143 pKa = 4.05LEE145 pKa = 4.46SYY147 pKa = 9.58PMEE150 pKa = 4.23VKK152 pKa = 9.72RR153 pKa = 11.84TVKK156 pKa = 10.46KK157 pKa = 10.92SLDD160 pKa = 3.44EE161 pKa = 4.43CSPEE165 pKa = 4.36DD166 pKa = 3.84IEE168 pKa = 4.4YY169 pKa = 10.59FKK171 pKa = 11.3NAVSNVPAIPSDD183 pKa = 3.52AYY185 pKa = 10.38EE186 pKa = 4.51DD187 pKa = 3.69EE188 pKa = 4.66CEE190 pKa = 4.09EE191 pKa = 4.45MEE193 pKa = 5.26DD194 pKa = 3.27DD195 pKa = 4.73TYY197 pKa = 11.59ILTYY201 pKa = 10.75DD202 pKa = 4.31DD203 pKa = 4.09EE204 pKa = 6.35DD205 pKa = 4.11EE206 pKa = 4.49EE207 pKa = 4.94EE208 pKa = 4.12EE209 pKa = 4.74DD210 pKa = 4.25EE211 pKa = 4.35EE212 pKa = 5.16EE213 pKa = 4.23EE214 pKa = 4.71DD215 pKa = 5.11EE216 pKa = 5.36EE217 pKa = 4.71MDD219 pKa = 5.41DD220 pKa = 4.1EE221 pKa = 4.56

Molecular weight: 25.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P0CAL9|VF93L_ASFWA Uncharacterized membrane protein KP93L OS=African swine fever virus (isolate Warthog/Namibia/Wart80/1980) OX=561444 GN=War-001 PE=3 SV=1
MM1 pKa = 7.41LFIWIKK7 pKa = 10.29NNFSISYY14 pKa = 9.74IPLIYY19 pKa = 10.56SLLQKK24 pKa = 6.97TTKK27 pKa = 10.24KK28 pKa = 10.24KK29 pKa = 9.74EE30 pKa = 3.57NRR32 pKa = 11.84FFIRR36 pKa = 11.84SGKK39 pKa = 10.05KK40 pKa = 8.51IQQIQMMMFITVYY53 pKa = 10.6DD54 pKa = 3.66INQKK58 pKa = 7.39QKK60 pKa = 10.6KK61 pKa = 9.02RR62 pKa = 11.84YY63 pKa = 8.47GLRR66 pKa = 11.84GCNLNLKK73 pKa = 8.85ATVLPLHH80 pKa = 6.39KK81 pKa = 10.31RR82 pKa = 11.84II83 pKa = 4.31

Molecular weight: 10.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

159

0

159

54328

26

2475

341.7

39.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.148 ± 0.159

2.109 ± 0.126

4.662 ± 0.114

6.304 ± 0.137

4.742 ± 0.121

4.305 ± 0.145

2.941 ± 0.12

8.375 ± 0.137

7.479 ± 0.204

10.369 ± 0.19

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.636 ± 0.08

6.043 ± 0.153

4.221 ± 0.171

4.211 ± 0.123

4.005 ± 0.138

6.023 ± 0.134

5.509 ± 0.118

5.032 ± 0.105

1.125 ± 0.049

4.76 ± 0.118
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski