Daphnia magna

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Crustacea; Branchiopoda; Phyllopoda; Diplostraca; Cladocera; Anomopoda; Daphniidae; Daphnia

Average proteome isoelectric point is 7.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 26600 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A164JBA2|A0A164JBA2_9CRUS Uncharacterized protein OS=Daphnia magna OX=35525 GN=APZ42_000886 PE=4 SV=1
MM1 pKa = 8.02CYY3 pKa = 10.28NKK5 pKa = 10.27EE6 pKa = 3.91EE7 pKa = 4.18QYY9 pKa = 11.75DD10 pKa = 3.81RR11 pKa = 11.84VICIGLFFLEE21 pKa = 4.58YY22 pKa = 9.84FQQHH26 pKa = 3.85QWEE29 pKa = 4.64DD30 pKa = 4.2FEE32 pKa = 6.58ASDD35 pKa = 3.95IASSVLDD42 pKa = 3.48YY43 pKa = 7.6MTNSFKK49 pKa = 10.85KK50 pKa = 10.58LRR52 pKa = 11.84DD53 pKa = 3.49RR54 pKa = 11.84PPDD57 pKa = 3.29NRR59 pKa = 11.84EE60 pKa = 3.46EE61 pKa = 4.03FTFANLMIAFEE72 pKa = 4.22FASDD76 pKa = 4.07YY77 pKa = 10.24VTGLQYY83 pKa = 10.09SEE85 pKa = 4.31RR86 pKa = 11.84RR87 pKa = 11.84SHH89 pKa = 7.51ILDD92 pKa = 3.11AHH94 pKa = 6.7EE95 pKa = 3.84SHH97 pKa = 6.14QFKK100 pKa = 10.39RR101 pKa = 11.84CLYY104 pKa = 9.57RR105 pKa = 11.84FLRR108 pKa = 11.84NDD110 pKa = 3.33RR111 pKa = 11.84RR112 pKa = 11.84WGLDD116 pKa = 3.13CQNFLHH122 pKa = 6.43MICRR126 pKa = 11.84CEE128 pKa = 5.6DD129 pKa = 3.46PLQYY133 pKa = 10.8LSRR136 pKa = 11.84PPNCAYY142 pKa = 10.12EE143 pKa = 4.24SDD145 pKa = 4.17EE146 pKa = 5.73DD147 pKa = 5.3DD148 pKa = 6.21SEE150 pKa = 6.85DD151 pKa = 5.9DD152 pKa = 6.29DD153 pKa = 6.79DD154 pKa = 7.3DD155 pKa = 6.24SDD157 pKa = 4.54NDD159 pKa = 4.96DD160 pKa = 4.58YY161 pKa = 12.13SDD163 pKa = 6.06DD164 pKa = 5.9DD165 pKa = 5.8DD166 pKa = 7.42DD167 pKa = 7.2SDD169 pKa = 6.45DD170 pKa = 6.0DD171 pKa = 5.75DD172 pKa = 7.03DD173 pKa = 6.62SDD175 pKa = 4.23SCSCVSDD182 pKa = 4.79DD183 pKa = 4.8GEE185 pKa = 4.56TDD187 pKa = 3.6GSVSGCSSCGYY198 pKa = 10.05HH199 pKa = 6.66VDD201 pKa = 4.03NGGPNDD207 pKa = 3.84NDD209 pKa = 3.89DD210 pKa = 4.48DD211 pKa = 5.17FDD213 pKa = 4.0VVRR216 pKa = 11.84FEE218 pKa = 5.91DD219 pKa = 4.04DD220 pKa = 2.92

Molecular weight: 25.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A164JX05|A0A164JX05_9CRUS Peptidase_M1 domain-containing protein (Fragment) OS=Daphnia magna OX=35525 GN=APZ42_000101 PE=4 SV=1
PP1 pKa = 7.9LLITRR6 pKa = 11.84QLTLLQATTLRR17 pKa = 11.84LPNTTQQRR25 pKa = 11.84HH26 pKa = 4.94RR27 pKa = 11.84STTQRR32 pKa = 11.84LTLPQVTTPQLVLRR46 pKa = 11.84TTWNLATTPQLMLLRR61 pKa = 11.84LTMLRR66 pKa = 11.84LLNTTPPRR74 pKa = 11.84LPSTQRR80 pKa = 11.84MLRR83 pKa = 11.84QVTIPKK89 pKa = 9.76PPSTTLLPMLLQFTTLKK106 pKa = 10.78LPNTTPLRR114 pKa = 11.84PLNTTLLQPRR124 pKa = 11.84SITPRR129 pKa = 11.84KK130 pKa = 9.96LSTSPHH136 pKa = 5.62LTQLQPTTLL145 pKa = 3.58

Molecular weight: 16.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

26599

1

26600

9136891

9

10574

343.5

38.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.854 ± 0.016

1.98 ± 0.017

5.13 ± 0.012

6.171 ± 0.023

4.005 ± 0.013

5.733 ± 0.022

2.578 ± 0.009

5.236 ± 0.013

5.623 ± 0.023

9.236 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.246 ± 0.009

4.57 ± 0.012

5.45 ± 0.024

4.432 ± 0.018

5.674 ± 0.013

8.633 ± 0.02

5.906 ± 0.019

6.361 ± 0.014

1.215 ± 0.006

2.813 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski